Biohazardous agent refers to an agent that is biological in nature, capable of self-replication, and has the capacity to produce deleterious effects upon biological organisms. Biohazardous agents include, but are not limited to; bacteria, fungi, viruses, rickettsiae, chlamydia, prion, parasites, recombinant products, allergens, cultured human and animal cells and the potentially biohazardous agents these cells may contain, infected clinical specimens, tissue from experimental animals, plant viruses, bacteria and fungi, toxins, and other biohazardous agents as defined by State and Federal regulations.
Biological waste is any material that contains or has been contaminated by a biohazardous agent. Biological waste includes, but is not limited to; Petri dishes, surgical wraps, culture tubes, syringes, needles, blood vials, absorbent material, personal protective equipment and pipette tips.
Sharps are items that are capable of puncturing, cutting or abrading the skin. Sharps include, but are not limited to; glass and plastic pipettes, broken glass, test tubes, razor blades, syringes, and needles. See
the Sharps and Infectious Waste: Handling and Disposal Guidelines for more details.
Biological Versus Chemical Waste
Biological waste must be managed separately from chemical waste. The most common example where chemical waste is mistaken for biological waste is agarose gel contaminated with ethidium bromide or heavy metals (i.e. arsenic, chromium). This type of material should always be managed as chemical waste. When both chemical and biological waste types exist, the biological agent(s) should be treated first. Once the biological agents have been deactivated by either autoclave or chemical disinfection, the remaining chemical waste should be submitted on a Hazardous Materials Pickup Request Form.
All sharps should be placed into properly labeled sharps containers or other rigid, puncture-proof containers. Make sure the container is sealed, labeled, and in-tact. Carefully place non-contaminated sharps next to the dumpster.
Contaminated sharps should be managed as follows:
- Biological contaminated sharps should be treated and managed following the Biological Waste Disposal and Pickup Procedures found at the bottom of this page.
- Chemical contaminated sharps should be submitted to REM on a Hazardous Materials Pickup Request Form.
- Biological and chemical contaminated sharps should be treated first as a biological waste. Once the biological agents have been deactivated by either autoclave or chemical disinfection, the remaining chemical waste should be submitted on a Hazardous Materials Pickup Request Form.
Liquid biological waste should be collected in containers for autoclaving or chemical disinfection. Autoclaved or chemically disinfected liquid wastes can be disposed via the laboratory sink. Do not pour melted agarose down the drain. Allow it to cool and solidify, then dispose of it as solid waste in biohazardous waste bags.
Solid biological waste, including solidified agarose gels, should be collected in appropriate biohazardous waste autoclave bags. Once the waste has been autoclaved or chemically disinfected, the autoclave bags should be taped or tied shut and placed inside of the cardboard box provided by REM.
Biological Waste Disposal and Pickup Procedures
Determine whether the biological waste is Category 1 or Category 2.
Category 1 biological waste includes any human-derived biological or substance known, assumed, or suspected of being infectious to humans, plants, or animals before treatment that may cause harm to the general public if released into the environment. Category 1 biological waste also includes any material contaminated with the aforementioned infectious substances and all items containing or contaminated with human blood or fluids. All Category 1 biological waste must be treated by autoclave or with an appropriate chemical disinfecting agent such as bleach prior to pickup.
Category 2 biological waste, also known as "look-alike waste", is non-infectious and includes material such as animal tissue, fluids, cell cultures and Petri dishes not fitting the Category 1 description. Category 2 waste does not require treatment.
- All solid biological waste, including sharps containers, must be placed into a cardboard box provided by REM. The box should be taped shut once it is full. Do not overfill the box; the box flaps should easily fold down onto the top of the box (See pictures below).
- Once you are ready to have waste removed from your laboratory, complete and sign a Bio-Materials Treatment and Certification Form and call REM at 49-40121 to schedule a pickup. Pickup usually occurs on the next working day. If necessary, regularly scheduled pickups can be setup throughout the week (i.e. pickup every Friday).
- A REM technician will come to your lab and remove the waste. The technician will leave behind the same number of boxes that were removed from the lab. It is the responsibility of the lab personnel to construct each new box.
REM will provide tape and one tape dispenser to each lab if necessary. REM will provide only one tape dispenser to each lab. If the tape dispenser is lost or damaged, it is your department's responsibility to replace it. Tape dispensers can be ordered from the Purdue University Concerto Office Products Catalog (Catalog Number: A8OM97805). Contact your business office manager for assistance if needed.
Once the waste is picked up from campus locations, it is transported back to Purdue's Laboratory Materials Storage Building where it is consolidated into a 40 cubic yard roll off. A waste vendor routinely picks up this roll off and transports it to a nearby Subtitle D non-hazardous waste landfill. The landfill requires that all waste be non-infectious, free of EPA regulated chemical waste, and contain no liquids.