Bio Safety
Biosafety Level 2 (BSL-2) Laboratory Practices
The following are basic rules for operating a laboratory at BSL-2. The Principal Investigator (PI) is responsible for the enforcement of these practices:
Training – The PI and lab staff must complete CITI Training every three years. PIs must ensure and document lab-specific biosafety training and demonstrated proficiency for lab personnel. The Bloodborn Patogens training is required if working with human source material or BBP (e.g. Hepatitis C virus, HIV).
Access – Keep labs closed and post a BSL-2 biohazard warning sign while infectious agents are in use. Store infectious agents in a lockable freezer or lab room.
Facilities – Chairs and other furniture are covered in a non-fabric, non-porous material that can not easily be contaminated. No carpet or rugs are permitted. An eyewash and a sink for hand washing must be available.
PPE – Wear appropriate PPE when working with infectious agents. Standard BSL-2 PPE includes a lab coat, gloves and eye protection. Other PPE may be required (see Purdue PPE Guideline).
Engineering controls – All procedures involving infectious materials that may generate an aerosol should be performed in a bio-contained environment using a certified Class II Biosafety Cabinet.
Decontamination – Decontaminate all cultures, stocks and biohazardous waste prior to disposal using an autoclave or chemical disinfection. Decontaminate work surfaces and lab equipment after work, spills or splashes.
Waste – Place biohazardous waste in a leak-proof secondary container labeled with a biohazard symbol. Collect sharps in a red plastic sharps container, and decontaminate the outside of container prior to transport.
Contact
Environmental Health and Safety
researchsafety@purdue.edu
765-494-6371