Virtual Lab: Separation and Spectroscopic Characterization of Caffeine from Coffee Samples
In this laboratory, students will see how to isolate caffeine from a cup of coffee and use spectroscopy to measure the amount of caffeine present.
Get the Lab Handout (teachers request answer key)
Lab Intro - Separation and Spectroscopic Characterization of Caffeine from Coffee Samples.
Pre Lab
Video 2: Introduction to UV-Vis Spectra
Video 3: The Difference Between Absorption Spectra and How They Really Look
Lab Procedure
Video 4: Procedure - Preparing the Coffee Sample
Video 5: Procedure - Extraction of Caffeine from the Coffee Sample
Video 6: Procedure - Preparing a Solution of Extracted Caffeine for Analysis
Data Analysis
Video 7: Data Analysis - UV-Visible Absorption Spectrum of Samples and Standards
This lab was created by the Ren Research group at Purdue University with funding from the National Science Foundation grant NSF CHE 1764347.