Sadikshya Aryal



Advisor's Name:

Val Watts

Email Address:

aryal0@purdue.edu

Training Group:

Membrane Biology

Research Summary:

Adenyl cyclase 1 (AC1), an effector of MOR, converts ATP to cAMP. Its activation in neurons increases cAMP levels, enhancing neuronal excitability and amplifying pain signals. Studies using a chronic inflammatory pain model in mice suggest AC1's contribution to chronic pain. Given its role in pain mechanisms, AC1 is a potential target for new pain medications. However, AC1's regulation in cellular systems is poorly understood due to a lack of suitable tools and models. My project aims to investigate two mechanisms underlying AC1 regulation: 1. AC1 regulation by Calcium/Calmodulin using a novel cellular model, and 2. AC1 homodimerization using NanoBRET, a proximity-based protein interaction system. Insights from this research will aid in developing therapeutic strategies to manage chronic pain and other conditions requiring AC1 modulation.

Why did you choose to attend PULSe:

I have an Undergraduate and Master’s degree in Agriculture/Plant Science. However, I later developed an interest in human health and pharmacological studies. Many PhD programs typically require applicants to have a specific academic background relevant to the field of study. However, PULSe stands out as an interdisciplinary program that aligns well with my diverse background and evolving interests. I applied to PULSe because it offers rotations through various labs within the life sciences program, allowing me to explore and deepen my understanding of human health and pharmacology. Additionally, PULSe provides the opportunity to benefit from both its resources and those of my home department. Why settle for just one when you can integrate the strengths of both for a richer academic experience?

About Me: (Hobbies and Interests when not in lab):

In my free time, I love dancing. I play some random songs on my TV and dance in my living room, letting the music guide my movements and lift my spirits. I also have a passion for cooking, with a special fondness for Nepali cuisine. I often invite friends over for dinner to share the joy of homemade meals and introduce them to the rich flavors of Nepal. My small backyard is my sanctuary during the summer, where I enjoy gardening. Currently, I am growing a variety of vegetables, including cucumber, okra, and tomatoes. The satisfaction of harvesting my produce and using it in my cooking adds an extra layer of fulfillment to my hobbies.