Purdue Imaging Facility

The Purdue Imaging Facility (PIF) is a research resource for Purdue faculty/staff/students and other academics and non-academics who want access to state-of-the-art light microscopy for their research and development work. PIF specializizes in Confocal and Multi-Photon Microscopy, Pre-clinical Bioluminescence/Fluorescence, Computed Tomography and Nuclear Imaging. In addition, we are a resource for researchers at Purdue University and biotech companies around the community. PIF is a member of the Indiana CTSI service core community. We provide the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals. All facility users receive individualized instrument training as well as project-specific advice for optimal data acquisition. Consultation on sample preparation, image rendering, and data analysis are also available as our knowledge base permits. Collaborations and contract research services are encouraged.

 

 

Zeiss 880 confocal - breast cancer cells captured by Aparna Shinde

Common Applications

 

Confocal microscopy

Our facility provides three visible-wavelength laser point scanning confocal systems suitable for creating multi-dimensional images of living or non-living samples.

  • Multi-colored fluorescence imaging
  • Photoablation/photoactivation
  • Imaging in aqueous environments
  • Material science – fluorescence and reflectance

 

Nikon Intravital MP system - Second Harmonic Generation - collagen matrix captured by Jesus Hermida

Multiphoton microscopy

If your sample is thicker than 100 µm or you require less phototoxicity to your living sample, you might benefit by using a multi-photon (MP)

  • Multi-dimensional images of thick samples, up to 800 microns
  • Live cell and live animal (intravital) imaging
  • Photo-uncaging
  • Photoablation/photoactivation
  • Second Harmonic Generation (non-centrosomatic)

 

Nikon TIRF system - virus particles captured by Monica Husby

TIRF microscopy

Total Internal Reflection Fluorescence uses a finite wave-front of energy to fluorescently illuminate samples and limit excitation of fluorophores close to the coverslip to dramatically increase the signal to noise of your sample.  

  • Protein dynamic studies on the cell membrane
  • Single molecule examinations
Nikon SR system - Structured Illumination Micrograph captured by Andy Schaber

Super Resolution microscopy

For spatial resolution beyond the diffraction-limit (<220 nm) we offer super resolution microscopy

  • Very high resolution intracellular imaging in thin samples
  • Detailed localization analysis
Nikon 90i system - Hematoxylin and Eosin and polarized illumination images captured by Andy Schaber

Wide-Field imaging

  • Cell morphology
  • Immunohistochemistry
  • Polarization
  • Amyloid assays

 

Spectral Instruments system - bioluminescence captured by Fanfei Meng

Pre-Clinical Molecular, microCT, and Nuclear imaging

The Bindley Imaging Facility provides systems capable of imaging larger subjects such as small rodents, medical devices or other non-biologics

  • Small Animal CT Imaging
  • Material science - Computed Tomography 
  • Fluorescent and Bioluminescent Imaging
NIS Elements and Zen software platforms

Image Processing and analysis for all data

Images are great to display but have so much more data. Use one of our commerical analysis software tools to pull out all the information.

  • Co-localization analysis
  • Intensity analysis
  • Time-lapse imaging of dynamic intracellular events
  • Three-dimensional morphology
  • Deconvolution