Bruker Luxendo Light Sheet Microscope

The lightsheet system is perfect for capturing 3D fluorescent images of organisms, tissue explants, and cell cultures over long periods. Basically, it shines a thin sheet of laser light on a slice of your sample, and high speed cameras take pictures of the glowing parts from the side. Using the light sheet method means less damage to your sample from laser exposure, which is great for imaging delicate things like embryos, larvae, and organoids. You can use it to image alive, preserved, or optically cleared samples.

-  Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses for illumination.

-  Two Olympus XLUMPLFLN 20x (eff. 22.2x) W 1.0 NA water immersion objective lenses for detection

-  Two high-speed Hamamatsu Orca Flash 4.0 V3 sCMOS cameras

-  Laser wavelength: 405 nm, 488 nm, 515 nm, 561 nm, 642 nm, 785 nm

-  Flexible light-sheet thickness (2 µm to 4 µm)

-  Filters:

• Bandpass 418-462, 457-501, 500-530, 526-564, 580-627, 655-704
• Longpass 498

-  Photomanipulation module: 532 nm ablation

For additional detailed specifications and sample preparation, please contact the facility manager.

Location: Hansen Lifescience Building B032