Biomolecular Interactions Instruments
MicroCal PEAQ-ITC
The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. |
MicroScale Thermophoresis (MST)
These two instruments perform biomolecular interaction studies based on the novel technology of microscale thermophoresis (MST). MST is the directed movement of particles in a microscopic temperature gradient. Bound species and un-bound species move in different speeds along the temperature gradient due to the changes in hydration shell of biomolecules caused by their structure/conformation changes upon binding, and these changes are quantified and used to determine binding affinities. MST allows measurement of interactions directly in solution (in any buffer, even in complex bioliquids) without the need of immobilization to a surface (immobilization-free). And in a lot of cases, the proteins don’t need to be labeled (Label-free). |
Octet RED384
The Octet® RED384 system is designed for increased throughput of label-free protein quantitation and kinetic characterization. One can get accurate concentration, kinetic constants, and affinity data for protein-protein, small molecule-protein and other molecular interactions – all with Dip and Read simplicity. The system utilizes ForteBio’s Bio-Layer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like cell culture supernatants and lysates. |
LightCycler 480 Real-Time PCR
This RT-PCR machine is utilized to perform Thermal Shift Assays, not only as a hit-validation tool to evaluate ligand – protein binding, but also a tool for fragment based drug screen. It accommodates 96- and 384-well plates. The unique Therma-Base technology enables consistent temperature distribution across the plate and rapid, accurate heat equalization, which results in higher throughput and reproducible data uniformity. |