Protein structure and function; X-ray crystallography; metalloenzymes; biodegradation of PCBs and related compounds
Structure and function of large protein complexes; Cryo-electron microscopy.
Membrane biophysics, membrane rapair, Linear and nonlinear optical microscopy, nanomedicine
Neuronal circuits in visual perception and learning Optogenetics Neurotechnology Autism Alzheimer's Disease Stroke
Membrane biochemistry, biophysics; structure-function of membrane proteins
Functional role deubiquitinating enzymes in cellular pathways implicated in neurodegeneration, such as Alzheimer's disease and Parkinson's disease
Chemical and systems biology as applied to drug discovery; design, synthesis, and evaluation of small molecule modulators of protein interactions; development and application of high content cell analysis screening platforms.
Protein-DNA interactions and protein engineering of homing endonucleases
Structural basis for RNA function
Macromolecular sequences and the evolution, structure and function of molecules; databases and computational tools for functional genomics
Multidrug resistance in human cancer
Synaptic and dendritic integration in vitro and in vivo, sensory integration, two-photon imaging, optogenetics, sub-cellular patch-clamp recordings, nanotechology, bioelectronics
method developments and applications of cryo-EM
Our lab focuses on acquiring and utilizing high throughput sequencing data (e.g. RNA-seq, ChIP-seq, ATAC-seq) to develop new computational models and biological assays to study genome regulation and human diseases, in particular immune related disorders and cancer. We are now working on the discovery and modeling of the regulatory circuitry of the non-coding genome which is essential for maintaining normal cellular physiology.
bioinformatics, computational biology
Biomechanics of cytoskeleton, cells and tissues; Computational modeling of biological structures
Stem cell biology; Muscle development and regeneration; Signaling regulation of satellite cells; Adipose stem cell; muscle-fat interaction
Viral gene expression; virus-host interactions; pathogenesis; virus receptors and virus assembly
Magnetic resonance imaging, image and signal processing,brain decoding and modeling
StructureFunction relationships of natural product biosynthetic enzymes for combinatorial biosynthesis
Development of targeted therapic and imaging agents for cancer and various inflammatory diseases. Function and molecular organization of the human red blood cell membrane. Novel methods for detection of human pathogens.
Understanding the regulation of phospholipase C enzymes in cardiovascular disease and cancer through macromolecular structure determination and functional assays.
Gene-to-Lead Drug Discovery
Structural biology, membrane proteins, protein folding, protein transport across membrane, protein import and trafficking, infectious diseases, pathogenic bacteria, multi-drug resistant bacteria, Gram-negative bacterial pathogens
System-wide Investigation of protein folding, energetics, and ligand binding
Computational chemistry and biological NMR
Protein misassembly diseases
Currently we are studying flavi viruses (e.g. Zika, dengue, yellow fever,) Common cold viruses, bacterial viruses and many other viruses
Entry of retroviruses and other enveloped viruses into cells; mechanism of enzymatic phosphoryl transfer
Development and application of NMR techniques with the focus on complex forms of molecular motion
Metabolic Engineering, Systems/Synthetic Biology, Protein Engineering, Microbial Ecology, Biofuels, -OMICS based approaches
1) Ebola virus and Marburg virus assembly and budding from the host cell plasma membrane.
2) Ceramide-1-phosphate and other sphingolipids signaling in cancers.
3) Zika virus and alteration of host cell lipid metabolism.
4) Disovery of new lipid-binding proteins.
Macromolecular structure and assembly using X-ray crystallography; membrane associated proteins; enzyme structure and function
Proteomics and biological mass spectrometry
We primarily study the molecular basis of GPCR-mediated signal transduction, principally via the techniques of X-ray crystallography and single particle electron microscopy. By determining atomic structures of signaling proteins alone and in complex with their various targets, we can provide important insights into the molecular basis of signal transduction and how diseases result from dysfunctional regulation. The lab is also interested in rational drug design and the development of biotherapeutic enzymes.
1) development of transiently-stable lipid- and polymer-based carrier systems for targeted drug & gene delivery; 2) development of high-throughput methods for detecting membrane protein activity; 3) development of materials and methods for controlled adsorption and templated protein crystallization
Structures and functions of DNA secondary structures as cancer-specific molecular targets; High-field NMR macromolecule structure determination; Structure-based rational drug design; DNA G-quadruplex secondary structures and their interactions with small molecule drugs and proteins; DNA-targeted anticancer drugs that inhibit transcription factors and topoisomerases.
Protein tyrosine phosphatases (PTPs), structure and function, PTP-mediated cellular signaling mechanisms, roles of PTPs in normal physiology and diseases, chemical biology and drug discovery
