Proteomics is the study of protein expression in cells or organisms. It gives a picture of an organism's physiology at a given time, and can tell us how organisms respond to their environment. Emerging mass spectrometry (MS)-based technologies allow an unprecedented view of spatial and temporal profiles of proteins and protein complexes in a biological system. The Purdue Proteomics Facility (PPF) provides a full proteomic infrastructure for state of the art characterization of proteins including their modifications and interactions. In the first part of my presentation, I will provide background information on various platforms available at the PPF for protein and peptide separation, and their analysis using highly sophisticated MS instruments. I will also discuss different quantitative and bioinformatics tools for proteomic studies.

 

Proteomics cores are commonly structured as a recharge facilities. But, this service model alone present unique challenges to fully realizing hypothesis driven research. New model is needed to meet the demands of a changing pedagogical environment and reduced financial resources. In the second part of my talk, I will discuss my vision and case examples for how we can develop PPF as a full-core research and instructional laboratory that provides new opportunities to prepare next generation of scientists in the changing landscape of education, energy and health care.