Lauren Robinson, Illumina, Inc. - “An introduction to home-brew library prep methods for Illumina sequencing and critical factors to ensure success: ChIP-Seq, ATAC-Seq, Hi-C, and CRISPR screening”

Bindley Bioscience CenterDiscovery Park
May 14, 2021
12:00 PM - 1:00 PM
Online - Zoom

Description

In this webinar we will discuss popular applications that utilize home-brew library preps with Illumina Sequencing as a downstream readout. This will include an overview of epigenetic methods designed to look at regions of open chromatin (ATAC-Seq), binding sites of DNA-associated proteins (ChIP-Seq/Cut and Run), and detection of long-range DNA interactions (Hi-C). Additionally, we will look at how Illumina Sequencing can be used as an effective strategy in genome wide CRISPR screens for quantification of sgRNAs as well as methods that allow the detection of transcriptional changes observed in the enriched or depletion knockout populations.

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Event Website

https://redcap.link/CTSI_Technology_Seminar

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