Agriculture News

August 1, 2019

New technology for protein complex discovery holds promise for biotechnology and crop improvement

Szymanski proteins Purdue University’s Daniel Szymanski developed a mass spectrometry method to identify the composition of protein complexes. Proteins that co-purify are predicted interactors. The profile data are generated from extracts isolated from developing Arabidopsis leaves. (Purdue Agricultural Communication photo) Download image

WEST LAFAYETTE, Ind. – Living cells survive and adapt by forming stable protein complexes that allow them to modulate protein activity, do mechanical work and convert signals into predictable responses, but identifying the proteins in those complexes is technically challenging. Purdue University researchers have developed a method to predict the composition of thousands of proteins complexes at one time, a discovery that will speed discoveries about cell functions.

The method predicts the composition of naturally occurring protein complexes that are extracted from living cells. It is significantly faster and cheaper than traditional methods that use large-scale cloning, affinity labels or antibodies to identify protein complex components. The method has the potential to help scientists understand how thousands of protein complexes function together to enable plant cells to grow normally and respond to changing environments.

Daniel Szymanski, a professor in Purdue’s Department of Botany and Plant Pathology, and graduate students Zach McBride and Youngwoo Lee, separated thousands of proteins based on size and charge and used mass spectrometry to predict which proteins were likely to bind to one another and form a stable protein complex. In this guilt-by-association approach, proteins that form a stable complex should co-purify with each other using any separation strategy.

Szymanski’s team also validated the process. The team confirmed the presence of many known and novel protein complexes that were predicted from the profiling method.

“From one of these separations, we get elution profiles for thousands of proteins,” said Szymanski, whose findings were published in the journal Molecular and Cellular Proteomics. “We can combine all of the protein profile data from the columns, identify the elution profiles that are most similar to each other and predict which proteins are physically associated with one another.”

Once protein complexes are identified, scientists can determine their function in cells, how cellular pathways are regulated, how those proteins affect cell signaling and more. Szymanski said the method works in any organism that has a sequenced genome, including corn, soybeans, rice and cotton.

“This method has been used to globally analyze protein complexes in plants of differing genotypes or those grown under different conditions. It’s like a new phenotyping tool to analyze systems-level changes in protein abundance, binding partners and subcellular localization,” Szymanski said.

The method serves as a large-scale hypothesis-generating machine that will accelerate understanding of the complicated workings of plant cells and give researchers broad knowledge about how plants adapt to heat, water and other stresses.

The research was supported by the National Science Foundation Plant Genome Research Program. 

Writer: Brian Wallheimer, 765-532-0233, bwallhei@purdue.edu

Source: Daniel Szymanski, 765-494-8092, szymandb@purdue.edu


ABSTRACT

A Label-Free Mass Spectrometry Method to Predict Endogenous Protein Complex Composition 

Zachary McBride1, Donglai Chen2, Youngwoo Lee1, Uma K. Aryal3, Jun Xie2, Daniel B. Szymanski1,4, 

  1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN
  2. Department of Statistics, Purdue University, West Lafayette, IN
  3. Purdue Proteomics Facility, Bindley Biosciences Center, Discovery Park, Purdue University, West Lafayette, IN
  4. Department of Biological Sciences, Purdue University, West Lafayette, IN

Information on the composition of protein complexes can accelerate mechanistic analyses of cellular systems. Protein complex composition identifies genes that function together and provides clues about regulation within and between cellular pathways. Cytosolic protein complexes control metabolic flux, signal transduction, protein abundance, and the activities of cytoskeletal and endomembrane systems. It has been estimated that one third of all cytosolic proteins in leaves exist in an oligomeric state, yet the composition of nearly all remain unknown. Subunits of stable protein complexes co-purify, and combinations of mass-spectrometry-based protein correlation profiling and bioinformatic analyses have been used to predict protein complex subunits. Because of uncertainty regarding the power or availability of bioinformatic data to inform protein complex predictions across diverse species, it would be highly advantageous to predict composition based on elution profile data alone. Here we describe a mass spectrometrybased protein correlation profiling approach to predict the composition of hundreds of protein complexes based on biochemical data. Extracts were obtained from an intact organ and separated in parallel by size and charge under non-denaturing conditions. More than 1000 proteins with reproducible elution profiles across all replicates were subjected to clustering analyses. The resulting dendrograms were used to predict the composition of known and novel protein complexes, including many that are likely to assemble through self-interaction. An array of validation experiments demonstrated that this new method can drive protein complex discovery, guide hypothesis testing, and enable systems-level analyses of protein complex dynamics in any organism with a sequenced genome.


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