Tuberculosis virulence factor identified, may be target for new drug
WEST LAFAYETTE, Ind. - Scientists have discovered the mechanism that hijacks the immune system's response to tuberculosis, revealing an important new drug target for the disease that kills more than 1 million people each year.
Herman Sintim, Purdue University's Drug Discovery Professor of Chemistry, collaborated with scientists at Johns Hopkins University to determine how tuberculosis turns off a human cell's signal to mount an immune response to the bacteria. Their findings were published in the journal Nature Chemical Biology.
Tuberculosis is a bacterial disease that results in coughing, fever, night sweats, weight loss and sometimes death.
When Mycobacterium tuberculosis enters a human cell, the presence of its DNA and a molecule that it makes called c-di-AMP alert the cell to the bacteria's presence. The human cell responds by creating a messenger molecule, cGAMP, which signals nearby cells to mount an immune response to kill the tuberculosis bacteria.
The human cell also produces another molecule, ENPP1, which degrades the cGAMP. That key step turns off the call for an immune response.
"Immune response can involve reactive oxygen and nitrogen species, which can kill the bacteria but at the same time cause collateral damage and also damage or kill the host cells as well," Sintim said. "There is a very delicate response to bacteria and stopping that response once bacteria have been taken care of."
But the tuberculosis bacterium has found a way to turn off the call for help. By producing a protein called cyclic dinucleotide phosphodiesterase (CdnP), the bacterium reduces the concentration of the cell's messenger molecule, cGAMP, a nucleic acid. This accentuates the effect of the human phosphodiesterase ENPP1, an enzyme that cleaves nucleic acids, to quickly degrade any already-made cGAMP and turn off the immune response early.
"The host cGAMP never gets to a high enough concentration to activate the immune response," Sintim said. "This is a very effective strategy the bacteria have developed to suppress an immune response."
Sintim and colleagues tested their hypothesis by creating a mutant of Mycobacterium tuberculosis that lacked the CdnP protein and tested it in a mouse model. On average, the mice with the mutant bacteria lived more than two times longer than mice with the wild type, suggesting that CdnP played a role in suppressing immune response.
They then artificially synthesized the cGAMP molecule and investigated if it was a substrate for CdnP. The CdnP degraded the human molecule as predicted.
Sintim said the CdnP protein in the tuberculosis bacteria now becomes an attractive target for a new drug. If a molecule could be developed that would inactivate or inhibit CdnP, it would improve immune response in tuberculosis patients.
Sintim's team identified several molecules that would bind with and inhibit CdnP, but they have not reached the potency level needed to create drugs. They will continue looking for new compounds that could potently inhibit this newly discovered CdnP drug target.
The National Institute of Allergies and Infectious Diseases, Howard Hughes Medical Institute, National Science Foundation, and Camille and Dreyfus Foundation funded the research.
Writer: Brian Wallheimer: 765-532-0233, firstname.lastname@example.org
Source: Herman Sintim, 765-496-6078, email@example.com
Inhibition of Innate Immune Cytosolic Surveillance by a M. tuberculosis Phosphodiesterase
Ruchi Jain Dey1,2,7, Bappaditya Dey1,2,6,7, Yue Zhen3,4,6,7, Laurene S. Cheung1, Jie Zhou3,4, David Sayre3, Pankaj Kumar1, Haidan Guo1, Gyanu Lamichhane1, Herman O. Sintim3,5, William R. Bishai1,2
1 Center for Tuberculosis Research, Johns Hopkins University, Baltimore, MD
2 Howard Hughes Medical Institute, Chevy Chase, MD
3 Department of Chemistry & Biochemistry, University of Maryland, College Park, MD
4 Department of Chemistry and Institute for Drug Discovery, Purdue University, West Lafayette, IN
5 Purdue Institute of Inflammation, Immunology and Infectious Disease, West Lafayette, IN
6 Present Address: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA; National Institutes of Health, Bethesa, MD
7 These authors contributed equally to this work.
Mycobacterium tuberculosis infection leads to cytosolic release of the bacterial cyclic dinucleotide (CDN) c-di-AMP and a host-generated CDN, cGAMP, both of which trigger type I interferon (IFN) expression in a STING-dependent manner. Here we report that M. tuberculosis has developed a mechanism to inhibit STING activation and the type I IFN response via the bacterial phosphodiesterase (PDE) CdnP, which mediates hydrolysis of both bacterial-derived c-di-AMP and host-derived cGAMP. Mutation of cdnP attenuates M. tuberculosis virulence, as does loss of a host CDN PDE known as ENPP1. CdnP is inhibited by both US Food and Drug Administration (FDA)-approved PDE inhibitors and nonhydrolyzable dinucleotide mimetics specifically designed to target the enzyme. These findings reveal a crucial role of CDN homeostasis in governing the outcome of M. tuberculosis infection as well as a unique mechanism of subversion of the host’s cytosolic surveillance pathway (CSP) by a bacterial PDE that may serve as an attractive antimicrobial target.