Current Projects

  • Chemical Resistance of Sea Lampreys to TFM
  • Toxicology of Nanosilver
  • Molecular Markers of Gonadal Development and Intersex
  • Combined Effects of Abiotic Stressors and Oil Contaminants
  • Poly- and Perfluoroalkyl Substances (PFASs)
  • Epigenetic Mechanisms of Atrazine

Past Projects

  • Temperature and Sex Differentiation in Fathead Minnows
  • Fish Diseases
  • Pharmaceuticals and Personal Care Products (PPCPs)
  • Function of miRNA’s in Daphnia pulex

Chemical Resistance of Sea Lampreys to TFM

Using a combination of toxicity tests coupled with next generation sequencing tools, we are studying whether sea lampreys have developed resistance to TFM which has been used to control lampreys in the Great Lakes for over 65 years.

TFM
Sea Lamprey

PI: Mark Christie
 
Funding: Great Lakes Fishery Commission

Toxicology of Nanosilver

We are evaluating the mechanisms of toxicity of nanosilver using zebrafish.

Silver nano particles from socks Silver nano particles from socks
Zebrafish larvee exposed to nano particles Zebrafish larvee exposed to nano particles
 

Publications:
Gao J et al. (2015) Nanosilver coated socks and their toxicity to zebrafish (Danio rerio) embryos. Chemosphere. 119:948-952. PubMed

 
Student: Jiejun Gao (PhD) 

 

Funding: China Scholarship Council and Department of Forestry and Natural Resources, Purdue University

Molecular Markers of Gonadal Development and Intersex

We are developing molecular (gene and protein) markers of gonadal development and intersex in teleosts using the Japanese medaka. Our strain (SK2MC) allows for early (~ 3 dpf) determination of gender since only males have white leucophores.

Molecular Markers of Gonadal Development and Intersex
Molecular Markers of Gonadal Development and Intersex
Publications: 
Abdel-Moneim A et al. (2015) Ovarian structure protein 1: A sensitive molecular biomarker of gonadal intersex in female Japanese medaka after androgen exposure. ET&C 34:2087-2094. PubMed
Abdel-Moneim A et al. (2015) Intersex in fishes and amphibians: Population implications, prevalence, mechanisms and molecular biomarkers. Journal of Applied Toxicology 35:1228-1240. PubMed
 
Student: Ahmed Abdel-Moneim (PhD)

 

Funding: Egyptian Government, Department of Forestry and Natural Resources, Purdue University

Combined Effects of Abiotic Stressors and Oil Contaminants

We are evaluating the combined effects of extreme dissolved oxygen, temperature, salinity and oil on growth and survival of Fundulus grandis.

Breeding Population
Gulf Killifish

 
Student: Jennifer Serafin(MS)
 
Funding: Gulf of Mexico Research Initiative

Poly- and Perfluoroalkyl Substances (PFASs)

The objectives of this work are several-fold: 1) Develop toxicity reference values for PFASs in amphibians; 2) Decipher the mechanisms of toxicity of PFASs using several in vitro and in vivo models; and 3) to evaluate the effects of mixtures.

Fluorotelomer sulfonate Fluorotelomer sulfonate
PFAS
PFAS Perfluorohexanoic sulfonate acid
PFAS
 
PhD Students: Gary Hoover and Amy Godfrey
 
Funding: Strategic Environmental Research and Development Program (SERDP), Integrative Graduate Education and Research Traineeship (IGERT), and Purdue Office of the Vice-President for Research

Epigenetic Mechanisms of Atrazine

Using the zebrafish, we are studying how a developmental exposure to atrazine results in later-in-life effects on reproduction.

Epigenetic Mechanisms of AtrazineAtrazine
Atrazine

Publications:
Wirbisky SE et al. (2015) Developmental origins of neurotransmitter and transcriptome alterations in adult zebrafish exposed to atrazine during embryogenesis. Toxicology 333:156-167. PubMed
Weber GJ et al. (2013) Transcriptome alterations following developmental atrazine exposure are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis. Toxicological Sciences 132:458-466. PubMed

PI: Jennifer Freeman

Funding: National Institute of Environmental Health Sciences

Temperature and Sex Differentiation in Fathead Minnows

We evaluated how temperature influences sex differentiation in fathead minnows. We induced non-directional sex reversal after exposing embryos and larvae from 0 h post fertilization to 45 days post hatch to fluctuating temperatures. Expression of hsp70 cycled with temperature.

Fathead Minnows
Fathead Minnows

Publications:
Coulter D et al. (2015) Fluctuating water temperatures affect development, physiological responses and cause sex reversal in fathead minnows. Environmental Science and Technology 49:1921-1928. PubMed
 
Student: David Coulter (PhD) (was co-advised with Tomas Höök)
 
Funding: Electric Power Research Institute (EPRI)

Fish Diseases

The objectives of this study was to determine the prevalence of diseases (virus, bacteria, and helminths) from exotic silver and bighead carps from the Wabash River, IN.

Diseases of Fishes


Student: Kensey Thurner (PhD) (major advisor: Reuben Goforth)
 
Funding: Indiana Department of Natural Resources (IDNR)

Pharmaceuticals and Personal Care Products (PPCPs)

We quantified PPCPs at the Baca Wildlife Refuge, CO and evaluated their impact to native fish such as the Rio Grande chub. We also conducted laboratory experiments with fathead minnows and examined mechanisms of toxicity of DEET, triclocarban, cotinine, and complex mixtures.

Triclocarban
Cleaning supplies


 
Publications:
Zenobio JE et al. (2015) Presence and effects of pharmaceutical and personal care products on the Baca National Wildlife Refuge, Colorado. Chemosphere 120:750-755. PubMed
Zenobio JE et al. (2014) Effects of triclocarban, N,N-diethyl-meta-toluamide and a mixture of pharmaceuticals and personal care products on fathead minnows (Pimephales promelas). Environmental Toxicology and Chemistry 33:910-919. PubMed
 
Student: Jenny Zenobio (MS)
 
Funding: United States Fish and Wildlife Service (USFWS) and Illinois-Indiana Sea Grant

Function of miRNA's in Daphnia pulex

We have identified and validated a set of miRNAs from D. pulex that are responsive to heavy metal exposure. 

miRNA
Daphnia pulex
Daphnia pulex
 
Publications:
Chen S et al. (2015) Novel cadmium responsive microRNAs in Daphnia pulex. Environmental Science and Technology 49:14605-14613. PubMed
Chen S et al. (2015) In silico prediction and in vivo validation of (Daphnia pulex) microRNAs. PLOS ONE DOI: 10.1371/journal.pone.0083708. PubMed
 
Student: Shaui Chen (PhD) (being co-advised by Krista Nichols)
 
Funding: Department of Forestry and Natural Resources, Purdue University