2022 Annual Meeting
November 9-11, 2022
We are excited to announce that registration for the 14th Center for Analytical Instrumentation Development (CAID) Workshop is open! This year we are hosting eight hands-on experiments with cutting-edge mass spectrometry technologies from three research groups (R. G. Cooks, J. Laskin, S. A. McLuckey) at Purdue University together with the Bindley Bioscience Center.
How it works?
The 14th CAID workshop will take place November 9th-11th at Purdue University (West Lafayette, IN).
Each experiment has a duration of about 25 minutes, and each visitor will typically be able to do six experiments in a session (ca. 3 hours), however registration options are available if a participant wants to explore all eight experiments. The workshop will take place across two different locations on campus: (i) the Department of Chemistry (R. B. Wetherill Laboratory of Chemistry -WTHR- and H. C. Brown Laboratory of Chemistry -BRWN-) and (ii) Discovery Park (Hall for Discovery and Learning Research -DLR- and the Bindley Bioscience Center -BIND-).
Several time blocks are available during the three workshop days to accommodate the participants while maintaining small groups to guarantee a hands-on experience. The days and time blocks, together with the experiments of interest for each participant, can be selected during registration.
What experiments will be demonstrated?
Here is a short description of the experiments we are hosting this year. All experiments will be guided by graduate students or research staff who directly work with or actually develop these technologies in a day-to-day basis.
- Chemical profiling of bacterial lysates by two-dimensional tandem mass spectrometry
Two-dimensional tandem mass spectrometry (2D-MS/MS) allows for the rapid acquisition of the full 2D data domain of a complex mixture in as little as ~1 second. This advancement in MS/MS analysis coupled with ambient ionization techniques improves analysis times by excluding a separation step and requiring minimal to no sample preparation. Participants will have the opportunity to analyze and observe difference in lipid profiles of different species of bacteria from lysates using 2D-MS/MS. Here we demonstrate how 2D MS/MS can provide information rich mass spectra of bacterial whole cell lysates and use the information to differentiate bacteria from one another by their lipid profiles alone. Additionally, we will also demonstrate how this technique could be used to differentiate between metabolic states using microwave irradiated lysates.
- Drug discovery using automated high-throughput DESI-MS
High throughput technologies have become increasingly important in current drug discovery workflows for both the screening of organic reactions and the development of biological assays. In this experiment participants will be able to have a first-hand experience with a novel high throughput technology based on desorption electrospray ionization mass spectrometry (DESI-MS). Participants will explore the abilities of an automated DESI-MS platform for the rapid (< 1 second per sample) assessment of enzymatic activity by testing multiple natural product sources under several conditions to identify an acetylcholinesterase inhibitor. Note that many options can be investigated during the experiment given that the overall analysis will take place in a matter of seconds.
- MRM profiling of suspect compounds in human saliva
The screening of drugs and metabolites in human biofluids is relevant for therapeutic monitoring, forensics, and disease diagnosis. Usually, mass spectrometry (MS)-based methods rely on time-consuming analytical workflows due to the use of liquid chromatography. Multiple reaction monitoring profiling is MS strategy to survey molecules based on their chemical functionalities and does not use sample separation by chromatography. This approach can be used to rapidly screen for the presence of hundreds of drugs and metabolites in human biofluids using 2 minutes of data acquisition and simple data processing. The small molecule screening can be followed by more elaborated validation methods that include product ion scans and sample purification/separation techniques. In this experiment, we will interrogate saliva samples for over 100 drugs and metabolites in under 2 minutes to discover which compound(s) have been spiked in the samples.
- Using a rotating wall mass analyzer to explore broad m/z ranges
Participants will have a first-hand experience with a custom soft-landing mass spectrometry instrument that uses a rotating wall mass analyzer (RWMA), a device that provides a uniform rotating electric field. The RWMA is a simple mass analyzer that separates a broad range of ions from low to extremely high m/z. When an ion beam passes through the device, different m/z ions deposit onto a surface with a ring-shaped pattern. Lower m/z ions deposit in rings with larger radii whereas larger m/z ions deposit in rings with smaller radii. The higher m/z range can be explored simply by lowering the frequency of the rotating electric field. During the workshop, participants of CAID will control the frequency of the RWMA and observe in real-time how this determines the radii of deposition of each m/z using an ion CCD detector.
- Accelerated Katritzky reaction at the gas/solution interface
Pyridinium salts are valuable structural units in medicinal chemistry and organic synthesis. These salts can be easily and rapidly synthesized through the Katritzky reaction between triphenylpyrilium (TPP) compounds and primary amines, a reaction that is significantly accelerated at gas/solution interface. In this experiment participants will explore the reaction acceleration phenomenon using a TPP salt and several amines. In the case of volatile amines (e.g. butyl amine), acoustically levitated droplets containing the TPP reactant will be exposed to amine vapor allowing an efficient gas/solution interfacial reaction (more than 50% conversion achieved after 1 minute). For non-volatile amines (e.g. p-anisidine), thin films containing both reactants, which are generated by drop casting and solvent evaporation, will be utilized to generate large surface-to-volume ratios and thus full conversion after 20 minutes.
- DESI-MS imaging of human fingerprints
The development of desorption electrospray ionization (DESI) on 2004 launched a whole new and widely applicable area in mass spectrometry (MS): ambient ionization. By mapping small molecule abundances and locations in tissue samples, it was possible to correlate morphology and molecular composition. Some applications of DESI include intraoperative diagnosis during brain cancer surgery and high-throughput chemical reaction screening, but there are important forensic challenges that have been addressed using chemical imaging too. In this demonstration, we will use the most recent DESI technology coupled to a high mass resolution mass spectrometer to image the fingerprinting of a volunteer. We will go through the workflow of analysis including sample preparation, instrument set-up, and data acquisition. While waiting for the ion images, we will discuss DESI imaging principles and show recent data acquired on the instrument for various types of samples.
- Simultaneous isolation of nonadjacent m/z ions using mirror switching in an electrostatic linear ion trap
Simultaneous isolation of ions of disparate mass-to-charge (m/z) ratios will be demonstrated via appropriately timed pulsing of entrance and exit ion mirrors in an electrostatic linear ion trap (ELIT) mass spectrometer. Simultaneous isolation takes advantage of both the rapid spatial separation of ions and the periodic nature of ion overlap in a closed path MR-TOF device. Mirror switching isolation pulses can be timed to coincide with overlapping events and because the ions of interest are spatially overlapped during the isolation event, they can be isolated from contaminant ions simultaneously. The time in which the overlap event occurs can be determined based on ion frequencies and beat periods within the device.
- Point-of-care methods analysis of biofluids using a miniature mass spectrometer
Current emphasis in drug and biomarker analysis is shifting towards point-of-care testing to empower clinicians and simplify healthcare delivery. While immunoassays and colorimetric tests have successfully been implemented for making on-site measurements, trace analysis is best done by mass spectrometry because of its wider molecular applicability and sensitivity. In this regard, miniature mass spectrometers are in a phase of rapid development to offer simple and powerful solutions for a wide variety of unmet biomedical needs. Additionally, improvements in ambient ionization methods and their combination with miniature mass spectrometers provide a particularly powerful solution for improving patient outcomes and reducing the healthcare burden on patients as well as clinicians. In this demo, drugs and biomarkers in blood and serum will be measured with the using paper spray ionization followed by mass analysis with a custom-built miniature mass spectrometer, the Mini-12.
Any questions?
If you have any questions, please contact Nicolás Morato (nmoratog@purdue.edu).
Click Here to Register!