PFAS analysis method in understudied complex media Agriculture Academic Year 2024 Accepted Environmental organic chemistry with various matrices Per- and polyfluoroalkyl substances (PFAS), manmade chemicals known for their toxicity and harmful impact on both human health and the environment, have been extensively researched, particularly the commonly detected perfluoroalkyl acids (PFAAs) like PFOS and PFOA found in soil and groundwater. However, there are thousands of chemicals within the PFAS family, and analyzing each individual chemical is time-consuming and cost-prohibitive. Also, analyzing PFAS in samples containing many other chemicals and high organic content has proven to be challenging with low chemical recoveries. We will test modified chemical analysis methodologies for a PFAS quantitative analysis to reduce complex matrix effects in biota caused by organic materials in the sample. the type of samples are including but not limited to soybean, corn, beef, milk, and fishes. Successful completion of this research will offer a robust analysis approach to evaluate PFAS so as to be conducive to enabling differentiation of precursor pools, improve bioaccumulation estimates, and elucidate in-vivo biota conjugation of PFAS. Youn Jeong Choi Ariana Jolie Lazo The work includes conducting various types of extractions, sample pretreatment using a freeze dryer and grinding, solvent handling, and further instrument utilization and data processing upon progress. Pretrained and work experience in Lee lab. 0 10 (estimated)