Purdue University
Publications (†Postdoc, *Grad student, ^undergraduate in the Iyer-Pascuzzi lab)
2023
*Caldwell D, Da Silva CR, McCoy AM, Avila H, Bonkowski JC, Chilvers MI, Helm M, Telenko D, Iyer-Pascuzzi AS. Uncovering the Infection Strategy of Phyllachora maydis during Maize colonization: A Comprehensive Analysis. bioRxiv, https://doi.org/10.1101/2023.08.26.554799
*Rivera-Zuluaga K, *Hiles R, †Barua P, *Caldwell D, Iyer-Pascuzzi AS. Getting to the root of Ralstonia invasion. 2023. Semin Cell Dev Biol. 148-149:3-12. doi: 10.1016/j.semcdb.2022.12.002. PMID: 36526528.
Yang C, Baireddy S, Méline V, Cai E, *Caldwell D, Iyer-Pascuzzi AS, Delp EJ. Image-based plant wilting estimation. Plant Methods. 2023 May 31;19(1):52. doi: 10.1186/s13007-023-01026-w. PMID: 37254098; PMCID: PMC10230817.
†Kumar N, *Caldwell C, Iyer-Pascuzzi AS. 2023. The NIN-LIKE PROTEIN 7 transcription factor modulates auxin pathways to regulate root cap development in Arabidopsis. Journal of Experimental Botany, 74(10):3047-3059. doi: 10.1093/jxb/erad058. PMID: 36787214.
†Méline V, *Caldwell D, Kim BS, Khangura RS, Baireddy S, Yang C, Sparks EE, Dilkes B, Delp EJ, Iyer-Pascuzzi AS. Image-based assessment of plant disease progression identifies new genetic loci for resistance. The Plant Journal, doi:10.1111/tpj.1610
2022
†Méline V, Hendrich CG, Truchon AN, *Caldwell D, *Hiles R, *Leuschen-Kohl R, *Tran T, Mitra RM, Allen C, Iyer-Pascuzzi AS. 2022.Tomato deploys defence and growth simultaneously to resist bacterial wilt disease. Plant Cell Environ. doi: 10.1111/pce.14456. PMID: 36213953.
Helm M, Singh R, *Hiles R, Jaiswal N, Myers A, Iyer-Pascuzzi AS, Goodwin S. 2022. Candidate effector proteins from the maize tar spot pathogen Phyllachora maydis localize to diverse plant cell compartments. Phytopathology, in press. doi: 10.1094/PHYTO-05-22-0181-R
*Tran T, *French E, Iyer-Pascuzzi AS. 2022. In vitro functional characterization predicts the impact of bacterial root endophytes on plant growth. Journal of Experimental Botany, 73(16):5758-5772. doi: 10.1093/jxb/erac228. PMID: 35596672.
Ingel B, *Caldwell D, Duong F, Parkinson D, McCulloh KA, Iyer-Pascuzzi AS, McElrone AJ, Lowe-Power TM. 2022. Revisiting the source of wilt symptoms: X-ray microcomputed tomography provides direct evidence that Ralstonia biomass clogs xylem vessels. Phytofrontiers, 2:41-51.
MacIntyre AM, †Méline V, Gorman Z, Augustine SP, Dye CJ, Hamilton CD, Iyer-Pascuzzi AS, Kolomiets MV, McCulloh KA, Allen C. 2022. Trehalose induces tomato defenses and increases drought tolerance and bacterial wilt disease resistance. PLoS One, 4:e0266254. doi: 10.1371/journal.pone.0266254
2021
Herrero-Huerta M, †Méline V, Iyer-Pascuzzi AS, Souza AM, Tuinstra MR, Yang Y. 2021. 4D Structural Root Architecture Modeling From Digital Twins by X-ray computed tomography. Plant Methods 7(1):123. doi: 10.1186/s13007-021-00819-1.
Denne NL, *Hiles RH, Kyrysyuk O, **Iyer-Pascuzzi AS, and **Mitra RM. 2021. Ralstonia solanacearum effectors localize to diverse organelles in Solanum hosts. Phytopathology, 111(12):2213-2226. doi: 10.1094/PHYTO-10-20-0483-R. (Denne, Hiles, Kyrysyuk are co-first authors; **Iyer-Pascuzzi and Mitra are co-corresponding authors)
French E, Kaplan I, Iyer-Pascuzzi AS, Nakatsu CH, and Enders. L. 2021. Emerging strategies for precision microbiome management in diverse ecosystems. Nature Plants. doi: 10.1038/s41477-020-00830-9. Online ahead of print.
Yang C, Baireddy S, Cai E, †Meline V, *Caldwell D, Iyer-Pascuzzi AS, Delp EJ. 2021. Image-Based Plant Wilting Estimation. arXiv:2105.12926. https://arxiv.org/abs/2105.12926
2020
Clark NM, Van den Broeck L, Guichard M, Stager A, Tanner HG, Blilou I, Grossmann G, Iyer-Pascuzzi AS, Maizel A, Sparks EE and Sozzani R. 2020. Novel Imaging Modalities Shedding Light on Plant Biology: Start Small and Grow Big. Annual Review of Plant Biology, 71. https://www.annualreviews.org/doi/pdf/10.1146/annurev-arplant-050718-100038
Kumari P, Ginzburg N, Sayas T, Saphier S., Bucki P, Miyara SB, *Caldwell DL, Iyer-Pascuzzi AS and Kleiman, M., 2020. A biomimetic platform for studying root-environment interaction. Plant and Soil, 447(1), pp.157-168. https://link.springer.com/content/pdf/10.1007/s11104-019-04390-6.pdf
†Kumar N and Iyer-Pascuzzi AS, 2020. Shedding the Last Layer: Mechanisms of Root Cap Cell Release. Plants, 9(3), p.308. https://www.mdpi.com/2223-7747/9/3/308
*French E, *Tran T and Iyer-Pascuzzi AS, 2020. Tomato Genotype Modulates Selection and Responses to Root Microbiota. Phytobiomes Journal, pp.PBIOMES-02. https://apsjournals.apsnet.org/doi/pdf/10.1094/PBIOMES-02-20-0020-R
Yang, C., Baireddy, S., Chen, Y., Cai, E., *Caldwell, D., †Méline, V., Iyer-Pascuzzi, A.S. and Delp, E.J., 2020, March. Plant Stem Segmentation Using Fast Ground Truth Generation. In 2020 IEEE Southwest Symposium on Image Analysis and Interpretation (SSIAI) (pp. 62-65). IEEE. https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9094605
Dundore-Arias JP, Eloe-Fadrosh EA, Schriml, LM, Beattie GA, Brennan FP, Busby PE, Calderon RB, Castle SC, Emerson JB, Iyer-Pascuzzi A.S. et al. 2020. Community-Driven Metadata Standards for Agricultural Microbiome Research. Phytobiomes Journal, 4(2), pp.115-121. https://apsjournals.apsnet.org/doi/pdf/10.1094/PBIOMES-09-19-0051-P
2019
*Caldwell D, and Iyer-Pascuzzi AS. A Scanning Electron Microscopy Technique for Viewing Plant− Microbe Interactions at Tissue and Cell-Type Resolution. Phytopathology (2019): PHYTO-07. https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-07-18-0216-R
Wang T, †Rostamza M, Song, Z, Wang, L, McNickle G, Iyer-Pascuzzi AS, Qiu Z, and Jin J. SegRoot: A high throughput segmentation method for root image analysis. Computers and Electronics in Agriculture 162 (2019): 845-854. https://www.sciencedirect.com/science/article/pii/S0168169919303874
2018
*French E, †Kim BS, ^Rivera-Zuluaga K, and Iyer-Pascuzzi AS. Whole root transcriptomic analysis suggests a role for auxin pathways in resistance to Ralstonia solanacearum in tomato. Molecular plant-microbe interactions 31, no. 4 (2018): 432-444. https://www.biorxiv.org/content/biorxiv/early/2017/08/16/176685.full.pdf
*French E and Iyer-Pascuzzi AS. A role for the gibberellin pathway in biochar-mediated growth promotion. Scientific reports 8, no. 1 (2018): 5389. https://www.nature.com/articles/s41598-018-23677-9.pdf
†Karve R, and Iyer-Pascuzzi AS. Further insights into the role of NIN-LIKE PROTEIN 7 (NLP7) in root cap cell release. Plant signaling & behavior 13, no. 1 (2018): e1414122. https://www.tandfonline.com/doi/pdf/10.1080/15592324.2017.1414122?needAccess=true
Mitchell K, *French E, Beckerman J, Iyer-Pascuzzi AS, Volenec J, and Gibson K. Biochar Alters the Root Systems of Large Crabgrass. HortScience 53, no. 3 (2018): 354-359. http://hortsci.ashspublications.org.ezproxy.lib.purdue.edu/content/53/3/354.full.pdf+html
2017
Balduzzi, M, Binder BM, Bucksch A, Chang C, Hong L, Iyer-Pascuzzi AS, Pradal C, and Sparks EE. Reshaping plant biology: qualitative and quantitative descriptors for plant morphology. Frontiers in plant science 8 (2017): 117. https://www.frontiersin.org/articles/10.3389/fpls.2017.00117/full
Bucksch A, Atta-Boateng A, Fortuné Azihou A, Battogtokh D, Baumgartner A, Binder B, Braybrook S, Chang C, Coneva V, DeWitt TJ, Fletcher AG, Gehan MA, Diaz-Martinez DH,Hong L, Iyer-Pascuzzi AS, Klein LL, Leiboff S, Li M, Lynch JP, Maizel A, Maloof J,Markelz C, Martinez CC, Miller L, Mio W, Palubicki W, Poorter H, Pradal C, Price CA,Puttonen E, Reese J, Rellán-Álvarez R, Spalding EP, Sparks EE, Topp CN, Williams J. “Morphological plant modeling: unleashing geometric and topological potential within the plant sciences.” Frontiers in plant science 8 (2017): 900. Morphological plant modeling: Unleashing geometric and topological potential within the plant sciences. Frontiers in Plant Science, 8:900. https://www.frontiersin.org/articles/10.3389/fpls.2017.00900/full
*Caldwell D, †Kim BS, and Iyer-Pascuzzi AS. Ralstonia solanacearum differentially colonizes roots of resistant and susceptible tomato plants. Phytopathology 107, no. 5 (2017): 528-536. https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-09-16-0353-R
Kim H, †Kim BS, Shim JE, Hwang S, Yang S, Kim E, **Iyer-Pascuzzi AS, and Insuk Lee. TomatoNet: a genome-wide co-functional network for unveiling complex traits of tomato, a model crop for fleshy fruits. Molecular plant 10, no. 4 (2017): 652-655. **co-corresponding author. https://www.cell.com/molecular-plant/pdf/S1674-2052(16)30294-5.pdf
2016
*French E, †Kim BS, and Iyer-Pascuzzi AS. Mechanisms of quantitative disease resistance in plants. In Seminars in cell & developmental biology, vol. 56, pp. 201-208. Academic Press, 2016.
†Karve R, *Suárez-Román F, and Iyer-Pascuzzi AS. “The transcription factor NIN-LIKE PROTEIN7 controls border-like cell release.” Plant physiology 171, no. 3 (2016): 2101-2111. http://www.plantphysiol.org/content/plantphysiol/early/2016/05/24/pp.16.00453.full.pdf
†Kim, B.S., *French, E., *Caldwell D., *Harrington, E.J. and Iyer-Pascuzzi, A.S. Bacterial wilt disease: Host resistance and pathogen virulence mechanisms. Physiological and Molecular Plant Pathology 95 (2016): 37-43. https://www.sciencedirect.com/science/article/pii/S0885576516300182
2015
†Karve, R., and Iyer-Pascuzzi, A.S. Digging deeper: high-resolution genome-scale data yields new insights into root biology. Current opinion in plant biology 24 (2015): 24-30. https://www.sciencedirect.com/science/article/pii/S1369526615000084
2014
Sozzani, R., and Iyer-Pascuzzi, A.S. Postembryonic control of root meristem growth and development. Current opinion in plant biology 17 (2014): 7-12. https://www.sciencedirect.com/science/article/pii/S1369526613001544
Cornell (graduate work) and Duke (postdoctoral work)
Primary research, reviews, and book chapters:
- Blair MS, Garris AJ, Iyer AS, Chapman B, Kresovich S, and McCouch SR. 2003. High resolution mapping and candidate gene identification of the xa5 locus for bacterial blight resistance in rice. Theor Appl Genet 107: 62 – 73. https://link.springer.com/content/pdf/10.1007/s00122-003-1231-2.pdf
- Iyer AS and McCouch SR. 2004. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol Plant Microbe Interact 17: 1348-135. https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI.2004.17.12.1348
- Iyer-Pascuzzi AS and McCouch SR. 2007. Functional markers for xa5 mediated resistance in rice (Orzya sativa L.). Mol Breeding 19: 291-296. https://link.springer.com/content/pdf/10.1007%2Fs11032-006-9055-9.pdf
- Iyer-Pascuzzi AS and McCouch SR. 2007. Recessive resistance genes and the Oryza sativa–Xanthomonas oryzae pv. oryzae pathosystem. Mol Plant Microbe Interact 20: 731-739. https://apsjournals.apsnet.org/doi/pdfplus/10.1094/MPMI-20-7-0731
- Iyer-Pascuzzi AS, Sweeney MT, N Sarla, and McCouch SR. 2007. Use of naturally occurring alleles for crop improvement. In: Upadhaya NM (ed) Rice Functional Genomics: Challenges, Progress and Prospects, pp 107-147. https://link-springer-com.ezproxy.lib.purdue.edu/content/pdf/10.1007%2F0-387-48914-2.pdf
- Iyer-Pascuzzi AS, Jiang H, Huang L, and McCouch SR. 2008. Genetic and functional characterization of the rice bacterial blight disease resistance gene xa5. Phytopathology 98: 289 – 95. https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-98-3-0289
- Iyer-Pascuzzi AS and Benfey PN. 2009. Transcriptional Networks in root cell specification. Biochem Biophys Acta 1789: 315-25. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691893/pdf/nihms113215.pdf
- Iyer-Pascuzzi AS, Simpson J, Herrera-Estrella L, and Benfey PN. 2009. Functional Genomics of root growth and development in Arabidopsis. Curr Opin Plant Biol 12: 165-71. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2683257/pdf/nihms107040.pdf
- Iyer-Pascuzzi AS*, Symonova O, Mileyko Y, Hao Y, Belcher H, Harer J, Weitz JS and Benfey PN. 2010. Imaging and analysis platform for automatic phenotyping and trait ranking of plant root systems. Plant Physiol 152: 1148-57 (*co-first author). http://www.plantphysiol.org/content/plantphysiol/152/3/1148.full.pdf
- Iyer-Pascuzzi AS and Benfey PN. 2010. Fluorescence-Activated Cell Sorting in Plant Developmental Biology. Methods Mol Biol 655: 313-319. https://link-springer-com.ezproxy.lib.purdue.edu/protocol/10.1007%2F978-1-60761-765-5_21
- Pop A, Huttenhower C, Iyer-Pascuzzi AS, Benfey PN, and Troyanskaya O. 2010. Integrated functional networks of process, tissue, and developmental stage specific interactions in Arabidopsis thaliana. BMC Systems Biol 4: 180. https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-4-180
- Iyer-Pascuzzi AS, Jackson TJ, Cui H, Petricka J, Busch W, Tsukagoshi H, and Benfey PN. 2011. Cell identity regulators link development and stress responses in the Arabidopsis root. Developmental Cell 21: 770-782.
- Cederholm HM, Iyer-Pascuzzi AS, and Benfey PN. 2012. Patterning the primary root in Arabidopsis. Wires Developmental Biology 1: 675-691. https://onlinelibrary.wiley.com/doi/pdf/10.1002/wdev.49
- Galkovskyi T, Mileyko Y, Bucksh A, Moore B, Symonova O, Price CA, Topp CN, Iyer-Pascuzzi AS, Zurek PR, Fang S, Harer J, Benfey PN, and Weitz JS. 2012. GiA Roots: Software for the High Throughput Analysis of Plant Root System Architecture. BMC Plant Biology 12:116. https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/1471-2229-12-116
- Iyer-Pascuzzi AS*, Zurek P, and Benfey PN. 2012. High-throughput, non-invasive imaging of root systems. Methods in Molecular Biology 959: 177 (*co-first author). https://www.researchgate.net/profile/Anjali_Iyer-Pascuzzi/publication/234089315_High-Throughput_Noninvasive_Imaging_of_Root_Systems/links/53d2b4550cf2a7fbb2e9aee4.pdf
- Fang S, Clark RT, Zheng Y, Iyer-Pascuzzi AS, Wietz JS, Kochian LV, Edelsburnner H, Liao H, and Benfey PN. 2013. Genotypic recognition and spatial responses by rice roots. Proc Natl Acad Sci USA 110(7): 2670-5. http://www.pnas.org/content/pnas/110/7/2670.full.pdf
- Topp CN, Iyer-Pascuzzi AS, Anderson JT, Lee CR, Zurek PR, Synomonva O, Zheng Y, Bucksh A, Mileyko Y, Galkovskyi T, Moore BT, Harer J, Edelsbrunner H, Mitchell-Olds T, Weitz JS, and Benfey PN. 2013. 3D phenotyping and quantitative trait locus mapping identify core regions of the rice genome controlling root architecture. Proc Natl Acad Sci USA 110(18):E1695-704. http://www.pnas.org/content/pnas/110/18/E1695.full.pdf