Porcine Reproductive and Respiratory Syndrome Virus (PRRS) ORF 5 Sequencing
Section: Molecular Diagnostics
Fee: $110.00
Turnaround Time: 3–7 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Porcine
Specimen
Name
Container
Minimum Amount
Serum
Red top vacutainer
1 ml
Virus isolate
Oral fluids
Sealed, Leakproof Plastic Conical Tube
2 ml
Lung
Sealed, Leakproof container
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
ORAL FLUID - Submit oral fluid in a leakproof container (i.e. plastic conical tube) with appropriate labeling.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
ORAL FLUID - Ensure the container is securely sealed and labeled.
Additional Information
This test is performed following a positive PRRS PCR test or virus isolation. Unless directed otherwise by submitter, the laboratory will sequence the sample with the lowest CT value, which should represent the sample with the most virus.- Indiana Animal Disease Diagnostic Laboratory Home
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