College of Veterinary Medicine

Mycobacterium paratuberculosis (Johne’s) PCR

Section: Molecular Diagnostics

Fee: $38.50 ($45.00 for pooled samples)

Turnaround Time: 2–4 business days

Days Test Started: Wednesday, Friday

Species: Bovine, Caprine, Ovine, Ruminant

Specimen

Name

Container

Minimum Amount

Feces

Leakproof container

8-10 grams


Fecal Swab

Viral Transport Medium/Leakproof Tube


Intestine

Leakproof Container or WhirlPak

2-3 Segments


Intestinal Contents

Leakproof Container or WhirlPak

2-5 ml


Colon

Leakproof Container or WhirlPak

2-3 segments


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leak proof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leak proof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap securely with paper towel or bubble wrap to provide protection.
FECES - Ensure container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information
For pooling, samples must be collected, identified and submitted individually. The laboratory will pool up to five samples per pool. If pooled sample yields a positive result, samples in that pool will be tested individually upon request for an additional charge.
Due to supply issues with one of the extraction kits, Johne’s PCR testing will be reduced to Wednesday and Friday until further notice.

Purdue University College of Veterinary Medicine, 625 Harrison Street, West Lafayette, IN 47907, 765-494-7607

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