Bovine Viral Diarrhea Virus (BVD) PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1 – 2 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Bovine, Caprine, Ovine, Ruminant
Specimen
Name
Container
Minimum Amount
Whole Blood
Purple top vacutainer
2 - 5 ml
Milk
Sealed, Leakproof Container
5 - 8 ml
Fetal Tissues
Sealed, Leakproof Container
Spleen
Sealed, Leakproof Container
2-3 samples
Lung
Sealed, Leakproof Container
2-3 samples
Intestine
Sealed, Leakproof Container
2-3 segments
Lymph Node
Sealed, Leakproof Container
2-3 samples
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in appropriate vacutainer tube making sure to meet minimum quantity.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit placenta (cotyledonary NOT amniotic).
MILK - Submit milk in a leakproof container (i.e. plastic conical tube) with appropriate labeling. REFRIGERATION - Refrigerate all samples until shipment.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
FETUS/FETAL TISSUES - Double bag body if shipping through a courier service. Ship placenta in a separate leakproof, plastic bag.
Additional Information
Also available as part of the Bovine Respiratory Virus Screen PCR (BRSV, IBR, PI3, BCV, BVD)For BVD ear notch testing, please see Bovine Viral Diarrhea Virus (BVD) PCR - Ear Notch Screen Pooled
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