College of Veterinary Medicine

Brucella abortus/suis (not B. ovis - see additional info)

Section: Serology

Fee: $3.50

Turnaround Time: 1–2 business days

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Bovine, Caprine, Equine, Porcine, Other

Specimen

Name

Container

Minimum Amount

Serum

Red top Vacutainer tube or equivalent

0.5 – 1 ml*


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. If multiple serology tests are requested on any one serum sample, minimum amount is 1-1.5 ml. Refrigerate specimen(s) until shipped.

Shipping Information

LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu. If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Ship specimens overnight with an ice pack. DO NOT SHIP OVER WEEKENDS OR HOLIDAYS.

Additional Information
Contact lab for large quantity submissions. Tests are started on an as-needed basis.
This test detects the presence of antibodies to Brucella abortus and Brucella suis.

**Other - Used for species from zoos, such as camelid and other species not listed - this does not include sheep for Brucella ovis testing. **

Brucella ovis testing is contracted to an accredited lab. Shipping fees will apply for samples forwarded to a different laboratory for testing. Samples will be charged at the rate of the contracted lab.

Available test methods for Brucellosis: BAPA and Card.

Export Submissions must state: destination, method of test, date of movement, and dilution. Tube tests require 48 hours and are contracted to NVSL.

Delay in results if confirmation testing at NVSL is needed.

Federal Form 4-33 Brucellosis Test Record forms are available free of charge from the Indiana State Board of Animal Health. Order online or contact BOAH: 317-544-2400 or by email at animalhealth@boah.in.gov.

Purdue University College of Veterinary Medicine, 625 Harrison Street, West Lafayette, IN 47907, 765-494-7607

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