Bovine Viral Diarrhea Virus (BVD) ELISA
Section: Virology
Fee: $6.60 - Rush Fee $50.00*
Turnaround Time: Up to 5 Business Days
Days Test Started: Friday
Species: Bovine
Specimen
Name
Container
Minimum Amount
Ear notch
Individual serum tube or 5mL Falcon tube
1cm x 1cm
Serum (>3 months of age)
Serum tube
1mL
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name. Collect ear notches with a sharp ear notching tool suitable for adult swine. Disinfect ear notchers between samples with 10% bleach. Number individual tubes. SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. Keep specimens chilled. Do not put samples in formalin.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak-proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues. SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
Additional Information
*This test detects acute and PI BVD. A definitive diagnosis that an animal is persistently infected (PI) can only be made by testing a positive animal a second time at least three weeks after the sample was taken. If the second sample is positive, the animal is persistently infected with BVD.* Samples must be received by 12:00 pm on Friday.
*Rush fee applies to tests started outside of the regularly scheduled testing day. If indicating Rush on the submission form, please include the date/time results are needed by.
*Contact lab for submissions greater than 50.
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