February 12, 2004
Engineers create chip-size version of widely used detector
WEST LAFAYETTE, Ind. - Researchers have created a portable, chip-size version of a detection system that is commonly used by industry and law enforcement to identify everything from agricultural toxins to DNA.
The miniature detector could move certain types of testing from the lab into the field, saving time and money while increasing security.
The team, which used a newly developed laser-processing technique to create the miniature detector, was supported by the National Science Foundation and led by a Purdue University engineer who conducted the work while he was at the University of California, Berkeley.
"Now we have a way of putting all of the critical components on one wafer," said Timothy D. Sands, the Basil S. Turner Professor of Engineering in the School of Materials Engineering and the School of Electrical and Computer Engineering at Purdue. "It's much the same in concept as going from separate transistors to an integrated circuit that includes many transistors on a single chip."
Findings about the miniature detector are detailed in a paper that has been posted online and that will appear March 1 in Sensors and Actuators A: Physical, an international journal published in Amsterdam by Elsevier B.V.
The paper was written by Sands and the following researchers from UC-Berkeley: J. Alex Chediak and Zhongsheng Luo, graduate students in materials science and engineering; Jeonggi Seo, a graduate student in applied science and technology; Nathan Cheung, a professor of electrical engineering and computer science; and Luke P. Lee, a professor of bioengineering.
The traditional fluorescence detection system works by attaching a fluorescent dye to specific molecules in a substance and then shining a laser onto the substance. The laser light is absorbed by the dyed molecules, causing them to emit a certain color, which is picked up by a sensor. The detection work normally is done using bulky, stationary equipment in a laboratory.
The new device, however, fits on a centimeter-wide chip, promising the development of miniature detectors that can be used in the field. Such portable instruments would be useful for a wide range of applications, from biologists doing basic research to farmers testing crops for toxins.
To create the chips, the team used a technique invented by Sands, Cheung and former graduate student William Wong, now a researcher at the Palo Alto Research Center in Palo Alto, Calif. This technique, known as "laser liftoff," uses a powerful laser to selectively separate and transfer thin-film components from one substrate to another to build up the successive layers of a "system-on-a-chip."
"We use lasers to manipulate materials, either to grow them or to process them," said Sands, who specializes in heterogeneous integration, or making devices by combining entirely different materials in new ways. "We can transfer films of materials from one substrate to another, and then use this laser-based assembly process to build up complex systems made of materials from different classes that are not normally compatible."
Fluorescence detection is commonly used in industry and science.
"It's the standard technique," Sands said. "The idea is that you tag a specific molecule or cell with a dye molecule that will emit light when it's excited. Then you illuminate your subject that's been tagged with the dye molecule, causing it to emit light at a longer wavelength."
The color of the laser is chosen to efficiently "excite" a specific dye. Shining a blue laser on a certain dye, for example, results in the emission of green light. A green laser might be chosen to excite a dye that emits red light.
DNA is tagged with a specific dye and then a fluid containing the tagged DNA is passed under a laser beam. The light-emission data are collected and analyzed, revealing information about the DNA.
"You also have to have some way of filtering the light," Sands said. "If, for example, you are using a laser that emits blue light, you can't allow the blue light to go into your photo-detector because it will wash out the signal from the green light the excited molecules emit.
"You have to filter out the blue light and just pick up the green."
The research team had to create a tiny filter that could fit on a chip.
The "light-emitting diode," or LED, that emits blue light is a thin layer of gallium nitride that's formed on top of a sapphire crystal. When electricity is passed through the LED, it produces a blue light.
With laser liftoff, the researchers use a device called an excimer laser to shine fast pulses of ultraviolet light onto the sapphire. Each pulse lasts only about 25 nanoseconds, or 25 billionths of a second. The concentrated energy removes the sapphire substrate on which the blue LED is formed, leaving behind only the thin layer. The LED film is transferred by the laser onto a filtering layer of cadmium sulfide, which screens out blue light. The layered diode and filter are then added to a photo detector on a single chip.
The thin-film LED is five micrometers or millionths of a meter, thick which is about one-twentieth as thick as a human hair, and is produced for less than $1, replacing the bulky and expensive laser in bench-top fluorescence detection instruments, Sands said.
"Something new that we recently reported was to put two colors, a blue and green LED, on one chip," he said.
Adding the green LED also meant adding another filter. Illuminating dyed molecules with a green LED causes the dye molecules to emit a reddish light. The second filter keeps green light from getting into the photo-detector so that it only detects the reddish light emitted by the excited dyed molecules.
At least two colors are considered critical for the analysis of biological and chemical materials.
"If you wanted to do biochemical detection of anthrax or some other substance, you almost always have to have two colors and your sample is tagged with two dyes," Sands said. "One serves as a control to precisely calibrate the measurement and the other color is for actually detecting the molecule you are after.
"We are now arguing that we can combine more than two colors, as well as arrays of LED-filter pairs. We could also build a spectrometer on a chip using an array of ultraviolet LEDs and a series of thin-film filters that absorb different colors."
The team is in the process of comparing the performance of the integrated microchip to bench-top instruments.
"Even if the performance never exceeds that of laser-based bench-top systems, the small size of the fluorescence detection microchip suggests a future as a portable, hand-held device for chemical detection and bioassay applications in remote locations," Sands said.
Writer: Emil Venere, (765) 494-4709, firstname.lastname@example.org
Sources: Timothy D. Sands, (765) 496-6105, email@example.com
J. Alex Chediak, (510) 643-5304, firstname.lastname@example.org
Zhongsheng Luo, (510) 643-7036, email@example.com
Sarah Yang, public information representative, UC-Berkeley, (510) 643-7741, firstname.lastname@example.org
Purdue News Service: (765) 494-2096; email@example.com
Related Web sites:
Heterogeneous Integration of CdS filters with GaN LEDs for Fluorescence Detection Microsystems
and Timothy D. Sands
1 Department of Materials Science and Engineering,
Microassembly of a hybrid fluorescence detection microsystem by heterogeneous integration of a CdS thin-film filter, an (In,Ga)N thin-film blue LED, and a disposable PDMS microfluidic device onto a Si PIN photodetector substrate is described. The CdS thin film filter was deposited directly onto a photodetector by pulsed-laser deposition. A thin-film (In, Ga)N LED was then transferred by a novel "pixel-to-point" laser lift-off process from the sapphire growth substrate to the silicon photodetector substrate. The final integrating step was achieved by positioning a disposable polymer microfluidic device onto the excitation/detection subsystem. Pixel-to-point transfer is potentially an enabling microassembly process for the fabrication of multicolor fluorescence-based bioassays and chemical detection microsystems.