Unusual sex chromosome creates third sex in Hessian flies

March 29, 2010

WEST LAFAYETTE, Ind. - Expectant human parents might wish for a boy or girl, but Hessian flies actually have a third option, and, no matter what, the flies are never surprised by the sex of their offspring.

Jeff Stuart, a Purdue University professor of entomology, and graduate student Thiago Benatti found that a sex chromosome in the mother predetermines the sex of Hessian fly offspring. The chromosome carries a gene called Cm (chromosome maintenance) that can create one of three basic sexes.

"When a mother carries this chromosome, she produces two types of females: female-producing females and male-producing females. Without the chromosome, the mother produces only males," Stuart said. "That means the species is composed of three different sex-related groups."

In humans, an egg contains an X chromosome, and a sperm contains an X or a Y. When sperm and egg unite, offspring with an XX combination become female and offspring with an XY combination become male. In Hessian flies, however, a female with the unusual chromosome, called W-prime (W') produces only females. Half of her offspring will carry the W' and produce only females, whereas the other half lacking the W' will produce only males.

Cm, one or more genes on the chromosome, has caused the evolution of the W' chromosome. It arose because the chromosome combination in Hessian flies isn't created at conception, as is the case with humans, but is established by chromosome elimination during embryonic development.

"I think that the mechanism of embryonic chromosome elimination is an evolutionary remnant of when this insect's ancestors were able to produce offspring without having sex," said Stuart, whose findings were released in the March issue of the journal Genetics.

Understanding the sex-determination process in the Hessian fly could lead to a way to control its populations or eliminate it altogether. The Hessian fly is the major pest of wheat crops, causing millions of dollars of damage annually.

Stuart said it might be possible to genetically modify Hessian flies using the Cm gene to eliminate the fly's ability to produce one of the sexes. Those modified flies could be introduced into fly populations to eliminate it as a pest.

"We're hopefully going to be able to manipulate this to bring the insect to its own demise," he said. "We may be able to use it to drive maladaptive traits into the population."

Stuart said the next step in the research would be to study the evolution of the Cm gene and determine the mechanisms it uses to control sex determination.

Stuart's research was funded by the U.S. Department of Agriculture National Research Initiative.

Writer: Brian Wallheimer, 765-496-2050, bwallhei@purdue.edu

Source: Jeff Stuart, 765-494-4561, stuartjj@purdue.edu

Ag Communications: (765) 494-8415;
Steve Leer, sleer@purdue.edu
Agriculture News Page



A Neo-Sex Chromosome That Drives Postzygotic
Sex Determination in the Hessian Fly

Thiago R. Benatti, Fernando H. Valicente, Rajat Aggarwal, Chaoyang Zhao, Jason G. Walling, Ming-Shun Chen, Sue E. Cambron,
Brandon J. Schemerhorn and Jeffrey J. Stuart

Two nonoverlapping autosomal inversions de?ned unusual neo-sex chromosomes in the Hessian ?y (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W9) chromosomes, where ZW9 females mate with ZZ males to engender female-producing (ZW9) and male-producing (ZZ) females in equal numbers. Genetic mapping and physical mapping identi?ed the inversions. Their distribution was determined in nine populations. Experimental matings established the association of the inversions with Cm and measured their recombination suppression. The inversions are the functional equivalent of the sciarid X-prime chromosomes. We speculate that W9 chromosomes exist in a variety of species that produce unisexual broods.