The Relative Costs of Sequencing Services at Purdue

The price of sequence data varies greatly between services. Most of this variation in price derives from cost of reagents required to run the instruments versus the actual amount of sequence generated by the instrument. But other costs, such as salaries for instrument operators, service contracts for the instruments and possibly instrument depreciation costs also contribute to the total cost of a service.

This table gives shows the relative costs of a number of services we offer at the Purdue Genomics Core Facility.

   Single Reads Paired-End Reads
SectionInstrumentChemistry800 (ish)1501005030025015010050
Cost per lane ($/lane)HiSeqRapid$1,511$1,222$897$2,591$1,993
$1,454
High Output$1,385$1,008$2,351$1,640
MiSeqStandard$2,293$1,669$1486
Nano$1,241$1051
3730XLLTL6.13
HTL961.625
HiSeqRapid$13$10$7$22$17$12
Cost per million read [pairs] ($/M reads)High Output$8$6$13$9
MiSeqStandard$100$128$65
Nano
3730XLLTL$6,130,000
HTL96$1,625,000

The table, while complex, basically says that in both cost/base of sequence and cost/sequence read that costs for Sanger sequence (3730XL) >> Illumina MiSeq > Illumina HiSeq.

 

How about the amount of time it takes for results to be returned after submission?

SectionInstrumentChemistrydays
Turn-Around TimeHiSeqRapid30
High Output30-90
MiSeqStandard14
Nano14
3730XLLTL1
HTL967

Here Sanger (3730XL) sequencing looks good. Further, “LTL” costs <$10/sample. It generates what is a vanishingly small amount of sequence data in comparison to next generation sequencing. But sometimes all you need is a single read.

If Sanger sequencing is more of a “single serving” option and a MiSeq run produces enough data to feed a family of four for a month, then is there not an intermediate service? Is there no service to sequence a plasmid, BAC or lambda clone for $20-$35?

 

 

 

 

 

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