Audrey Ruple

Audrey Ruple

Associate Professor of One Health Epidemiology
Office: Matthews Hall, Room 220
Phone: (765) 496-0414

Research Interests:

Dr. Ruple’s primary research interest lies in the area of “One Health,” the intersection of human, animal, and environmental health. Her diverse and extensive experiences lend themselves to her One Health research in both comparative/translational medicine and infectious diseases that occur at the human-animal interface. Dr. Ruple’s comparative medicine research is primarily focused on the companion dog as a model for health and disease in human populations. This model system has many advantages over other animal models due to the fact that companion dogs share our environment in addition to a large amount of shared ancestral genetic sequence. Dogs also share much of the disease burden seen in human populations, especially in regards to cancer outcomes, and can be used as a model for healthy aging.

Dr. Ruple is also an expert in infectious diseases, biosecurity, and hospital infection control. Her veterinary background is especially important in this research area given recent estimates that up to 75% of emerging infectious diseases in human populations are zoonotic, meaning they arise from animal populations. Several of her published works on hospital infection control and biosecurity have received awards from international organizations such as the International Society for Disease Surveillance.

Dr. Ruple is a member of the Purdue Comparative Oncology Program and Purdue Center for Cancer Research, acts as the Advisor to the Purdue One Health Club, and serves as the Chair of the Senate’s Equity and Diversity Committee.

In the News:


Doctor of Philosophy (PhD), Cell and Molecular Biology, Specialization in Cancer Biology, Colorado State University (2014)

Master of Science (MS), Epidemiology, Colorado State University (2011)

Doctor of Veterinary Medicine (DVM), Colorado State University (2008)

Bachelor of Science (BS), Microbiology, minor concentrations in Anatomy and Neurobiology, Colorado State University (2002)


Certification in Leadership for Veterinary Professionals, National Health Service Leadership Academy and Royal College of Veterinary Surgeons, 2018

Member, Royal College of Veterinary Surgeons (MRCVS), 2016

Certification in Diversity and Inclusion, Center of Excellence for Diversity and Inclusion in Veterinary Medicine, 2015

Graduate Teaching Certification, The Institute for Learning and Teaching, 2014

Diplomate, American College of Veterinary Preventive Medicine (DipACVPM), 2013

Veterinary Accreditation, United States Department of Agriculture (NVAP), 2008

Veterinary Licensure, State of Colorado, number 8803 (Active), 2008

Selected publications:

Comparative/Translational Medicine

Selmic LE, Ruple A. A systematic review of surgical margins utilized for removal of cutaneous mast cell tumors in dogs. BMC Veterinary Research, 2020; 16(5). DOI: 10.1186/s12917-019-2227-8

Tuohy JL, Shaevitz MH, Garrett LD, Ruple A, Selmic LE. Demographic characteristics, site and phylogenetic distribution of dogs with appendicular osteosarcoma: 744 dogs (2000-2015). PLOS ONE 14(12): e0223243. DOI: 10.1371/journal.pone.0223243

Cray M, Selmic LE, Ruple A. Salivary neoplasia in dogs and cats: 1996–2017. Veterinary Medicine and Science, 2019; 00:1-6. DOI: 10.1002/vms3.228

Szigetvari N, Dhawan D, Ramos-Vara J, Leamon C, Klein P, Ruple A, Heng H, Pugh M, Rao S, Vlahov I, Deshuillers P, Low P, Fourez L, Cournoyer A, Knapp D. Phase I/II clinical trial of the targeted chemotherapeutic drug, folate-tubulysin, in dogs with naturally-occurring invasive urothelial carcinoma.  Oncotarget, 2018. DOI: 10.18632/oncotarget.26455

Ambrosius LA, Dhawan D, Ramos-Vara JA, Ruple A, Childress MO. Quantification and prognostic significance of programmed cell death ligand 1 in canine diffuse large B-cell lymphoma. American Journal of Veterinary Research, 2018; 79(6): 643-649.  DOI: 10.2460/ajvr.79.6.643

Selmic LE, Ryan SD, Ruple A, Pass W, Withrow SJ.  Tibial plateau leveling osteotomy increases the risk of proximal tibial osteosarcoma in dogs: A case-control study.  Journal of American Veterinary Medical Association, 2018; 253(6): 752-756.  DOI: 10.2460/javma.253.6.752

Childress MO, Ramos-Vara, JA, Ruple A. Retrospective analysis of factors affecting clinical outcome following CHOP-based chemotherapy in dogs with primary nodal diffuse large B-cell lymphoma.  Veterinary and Comparative Oncology, 2018; 16(1): E159-E168.  DOI: 10.1111/vco.12364.

Childress MO, Ramos-Vara JA, Ruple A.  A randomized controlled trial of the effect of prednisone omission from a multidrug chemotherapy protocol on treatment outcome in dogs with peripheral nodal lymphomas.  Journal of American Veterinary Medical Association, 2016; 249(9): 1067-1078. DOI: 10.2460/javma.249.9.1067.

Ruple A, Avery AC, Morley PS. Differences in the geographic distribution of B-cell and T-zone lymphomas in Golden retrievers in the United States. Veterinary and Comparative Oncology, 2016; DOI: 10.1111/vco.12258.

Ruple A, Morley PS.  Risk factors associated with development of histiocytic sarcoma in Bernese mountain dogs.  Journal of Veterinary Internal Medicine, 2016: 30(4): 1197-1203.  DOI: 10.1111/jvim.13964.

Knapp DW, Ruple A, Ramos-Vara JA, Naughton JF, Fulkerson CM, Honkisz SI.  A nonselective cyclooxygenase inhibitor enhances the activity of vinblastine in a naturally-occurring caning model of invasive urothelial carcinoma.  Bladder Cancer, 2016: 2(2): 241-250.  DOI: 10.3233/BLC-150044.

Biosecurity and Infectious Diseases

Stella J, Hurt M, Bauer A, Gomes P, Ruple A, Beck A, Croney C. Does flooring substrate impact kennel and dog cleanliness in commercial breeding facilities? Animals, 2018. 8(4): 59. DOI: 10.3390/ani8040059.

Goeman V, Tinkler SH, Hammac GK, Ruple A.  Evaluating environmental sampling methods for detection of S. enterica in a large animal veterinary hospital.  Canadian Veterinary Journal, 2018. 59(4): 408-412.

Widmar NJO, Dominick SR, Tyner WE, Ruple A. When is genetic modification socially acceptable? When it is used to advance human health through avenues other than food and nutrition. PLOS One, 2017; 12(6).  DOI: 10.1371/journal.pone.0178227.

Widmar NJO, Dominick SR, Ruple A, Tyner WE.  The influence of health concern on travel plans with focus on the Zika Virus in 2016. Preventive Medicine Reports, 2017; 6: 162-170. DOI: 10.1016/j.pmedr.2017.02.023.

Robinette C, Saffran L, Ruple A, Deem SL.  Zoos and Public Health: A partnership on the One Health frontier.  One Health, 2016; 3: 1-4. DOI: 10.1016/j.onehlt.2016.11.003.

Ruple-Czerniak A, Bolte D, Burgess B, Morley PS.  Comparison of two sampling and culture systems for detection of Salmonella enterica in the environment of a large animal hospital.  Equine Veterinary Journal, 2014; 46(4): 499-502.  DOI: 10.1111/evj.12193.

Ruple-Czerniak A, Aceto H, Bender JB, Paradis MR, Shaw SP, Van Metre DC, Weese JS, Wilson DA, Wilson J, Morley PS.  Syndromic surveillance for evaluating the occurrence of healthcare-associated infections in equine hospitals.  Equine Veterinary Journal, 2014; 46(4): 435-440.  DOI: 10.1111/evj.12190.

Ruple-Czerniak A, Aceto H, Bender JB, Paradis MR, Shaw SP, Van Metre DC, Weese JS, Wilson DA, Wilson J, Morley PS.  Using syndromic surveillance to estimate baseline rates for healthcare-associated infections in critical care units of small animal referral hospitals.  Journal of Veterinary Internal Medicine, 2013; 27(6): 1392-1399.  DOI: 10.1111/jvim.12190.