Interdisciplinary Life Science - PULSe Great research is a matter of choice

Yu Xia

Yu Xia Profile Picture

Assistant Professor

Contact Info:

Training Group(s):
Membrane Biology

Current Research Interests:

Research in the Xia Laboratory is focused on mass spectrometric method and tool development to achieve fast, sensitive, and confident structural characterization of biomolecules.  Dr. Xia’s group has taken a unique approach by utilizing radical reactions toward biomolecules to harness high-level structural information including C-C double bond location in lipids, protein disulfide bond linkage patterns, and the stereochemistry of sugar units within carbohydrates. Radical reactions are also used to synthesize protein radicals in gas phase. Their intrinsic chemical properties including intra- or inter molecular reactivity and radical migration are further probed by mass spectrometry.

Selected Publications:

1. Kirt L. Durand, Xiaoxiao Ma and Yu Xia, "Intra-molecular Reactions between Cysteine Sulfinyl Radical and a Disulfide Bond within Peptide Ions",  Int. J. Mass Spectrom., Submitted,

2. C. A. Stinson and Y. Xia*, “Reactions of Hydroxyalkyl Radicals with Cysteinyl Peptides in a NanoESI Plume”, J. Am. Soc. Mass Spectrom., 2014, DOI:10.1007/s13361-014-0898-8

3. C. Konda, F. Londry, B. Bendiak, and Y. Xia*, "Obtaining Stereo-Structural Information with Single-Sugar Resolution from Linear Oligosaccharides by Mass Spectrometry", J. Am. Soc. Mass Spectrom., 2014,. DOI: 10.1007/s13361-014-0881-4

4. X. Ma and Y. Xia*, “Pinpointing Double Bonds in Lipids by Paternò–Büchi Reactions and Mass Spectrometry”, Angew. Chem., Int. Ed, 2014, 53, 2592-2596.

5. K.L. Durand, X. Ma, and Y. Xia*, "Intra-molecular Reactions as a New Approach to Investigate Bio-Radical Reactivity: A Case Study of Cysteine Sulfinyl Radical", Analyst, 2014, 139, 1327-1330 “Featured as hot article”

6. L. Tan, H.Hu, J.S. Francisco*, and Y. Xia*, "A Mass Spectrometric Approach for Probing the Stability of Bioorganic Radicals", Angew. Chem., Int. Ed., 2014, 53, 1887-1890 “Hot paper” and “Featured as front cover of issue 7, Feb 10, 2014”

7. C. Konda, B. Bendiak, and Y. Xia*, "Linkage Determination of Linear Oligosaccharides by MS n (n >2) Collision-Induced Dissociation of Z 1 Ions in Negative Ion Mode”, J. Am. Soc. Mass Spectrom., 2014, 25, 248-257

8. Z. Lin, L. Tan, S. Garimella, L. Li, T. Chen, W. Xu, Y. Xia*, and Z. Ouyang*, "Characterization of a DAPI-RIT-DAPI System for Gas-Phase Ion/Molecule and Ion/Ion Reactions", J. Am. Soc. Mass Spectrom., 2014, 25, 48-56.

9. L. Tan, K. L. Durand, X. Ma, and Y. Xia*, "Radical Cascades in Electron Transfer Dissociation (ETD) - Implications for Characterizing Peptide Disulfide Regio-Isomers", Analyst, 2013, 138, 6759-6765.

10. K. L. Durand, X. Ma, C.E. Plummer, and Y. Xia*, "Tandem Mass Spectrometry (MS n) of Peptide Disulfide Regio-Isomers via Collision-Induced Dissociation: Utility and Limits in Disulfide Bond Characterization", Int. J. Mass Spectrom., 2013, 343-344, 50-57.

11. C. A. Stinson and Y. Xia*, “Radical Induced Disulfide Bond Cleavage within Peptides via Ultraviolet Irradiation of an Electrospray Plume”, Analyst, 2013, 138, 2840-2846.

12. C. B. Love, L. Tan, J. S. Francisco*, and Y. Xia*, “Competition of Charge- vs. Radical-Directed Fragmentation of Gas-Phase Protonated Cysteine Sulfinyl Radicals”, J. Am. Chem. Soc., 2013, 135, 6226–6233.

13. L. Tan and Y. Xia*, "Gas-Phase Reactivity of Peptide Thiyl (RS•), Perthiyl (RSS•), and Sulfinyl (RSO•) Radicals Formed from Atmospheric Pressure Ion/Radical Reactions", J. Am. Soc. Mass Spectrom., “Distonic Ion Focus Issue”, 2013, 24, 534-542.

14. L. Tan and Y. Xia*, "Gas-Phase Peptide Sulfinyl Radical Ions: Formation and Unimolecular Dissociation", J. Am. Soc. Mass Spectrom., 2012, 23, 2011-2019.

15. C. Konda, B. Bendiak, and Y. Xia*, “Differentiation of the Stereochemistry and Anomeric Configuration for 1-3 Linked Disaccharides via Tandem Mass Spectrometry and 18O-labeling", J. Am. Soc. Mass Spectrom., 2012, 23, 347-358.

16. S. R. Cole, X. Ma, X. Zhang, and Y. Xia*, "Electron Transfer Dissociation (ETD) of Peptides Containing Intramolecular Disulfide Bonds", J. Am. Soc. Mass Spectrom., 2012, 23, 310-320.

17. X. Ma, C. B. Love, X. Zhang, and Y. Xia*, " Gas-Phase Fragmentation of [M + nH + OH] n•+ Ions Formed from Peptides Containing Intra-Molecular Disulfide Bonds", J. Am. Soc. Mass Spectrom., 2011, 22, 922-930.

18. W. Xu, N. Charipar, M. A. Kirleis, Y. Xia, Z. Ouyang* "Study of Discontinuous Atmospheric Pressure Interfaces for Mass Spectrometry Instrumentation Development", Anal. Chem., 2010 , 82, 6584-659219.   H. Wang, Z. Ouyang, Y. Xia*, "Peptide Fragmentation during Nanoelectrospray Ionization", Anal. Chem., 2010 , 82, 6534-6541.

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