Interdisciplinary Life Science - PULSe Great research is a matter of choice

Michael D. Kane

Michael D. Kane Profile Picture

Assistant Professor of Computer & Information Technology. Lead Genomic Scientist, Bindley Bioscience Center, Discovery Park
Ph.D. 1996 Purdue University

Contact Info:

Training Group(s):
Chromatin and Regulation of Gene Expression

Current Research Interests:

My research involves the development and utilization of biotechnology and discovery support systems in the life sciences. This includes the use of DNA microarray technology to investigate the genomic basis of disease, development and drug toxicity. The following is a list of collaborative projects in the lab (as of 7/2005).

Molecular Basis of Ecstasy-Induced Hyperthermia. In collaboration with Dr. Jon Sprague (Virginia Tech) and Dr. Daniel Rusyniak (Indiana University Medical School), Dr. Kane is leading this effort and employing genomic techniques to study ecstasy, a recreational drug of abuse, which has clinically-relevant toxicity manifested as hyperthermia and kidney failure. Using the rat as a model of ecstasy toxicity, the project is discovering specific genes that are differentially regulated in target tissues to develop an effective therapeutic intervention for human ecstasy overdose.

Metabolic Engineering of S. Cerevisiae Glycolytic Pathway for the Production of Lactic Acid. This project involves the genetic engineering of yeast for the bioproduction of lactic acid at the industrial scale without ethyl alcohol contamination, used for biodegradable polymer synthesis. This project is led by Dr. Nancy Ho of the Laboratory for Renewable Resources Engineering.

Multiplexed Detection of Foodborne Pathogens and Bioterror Threat Agents using Fluorescence Resonance Energy Transfer in a Spatial Detection Format. This project is led by Dr. Bruce Applegate (Food Science) involves the development of a rapid, on-site assay platform for the detection of foodborne pathogens, through the detection of specific genes. Preliminary results show the detection platform is sensitive, gene specific and can be performed within 10 minutes.

Systems Analysis of Multiply Stress in Arthobacter. This project aims to characterize the genomic/genetic basis of survival of a bacterium that thrives in dehydrated radioactive soils, and its potential role in bioremediation of land contaminated with radioactivity. Preliminary data generated in Dr. Kane’s laboratory has assisted in the identification of a specific gene region that confers resistance to heavy metal toxicity in this organism.

Genetic and molecular insights into mechanisms underlying a maize disease. This project is using maize microarrays to investigate the pathogen response processes in maize to a fungal toxin, looking at both sensitive and resistant strains in collaboration with Dr. Gurmukh Johal (Botany and Plant Pathology).

Selected Publications:

Kane, M.D. Technical Issues in DNA Microarray Production and Utilization: Impact on Clinical Research. Expert Review of Molecular Diagnostics (in press), 2005.

Kane, M.D. and Brewer, J.L. An Information Technology Emphasis in Biomedical Informatics Education. Journal of the American Medical Informatics Association (submitted), 2005.

Chirnomas, R.B., Jeffy, B.D., Ranganathan, R., Sahay, N., Kane, M.D., Payne, C.M. and Romagnolo, D. Toxicogenomics of exposure to polycyclic aromatic hydrocarbons: protective effects of the synthetic alpha-naphthoflavone. Journal of Nutrition (submitted), 2004.

Kane, M.D. Aligning experimental design with bioinformatics analysis to meet research objectives. Cytometry 47: 50-51, 2002.

Kane, M.D. Screening for xenobiotic-mediated P450 induction using a dedicated DNA microarray protocol. Journal of Clinical Ligand Assay 24: 149-151, 2001.

Sprague, J.E., Worst, T.J., Haynes, K., Mosler, C.R., Nichols, D.E. and Kane, M.D. The pharmacodynamic characterization of an antisense oligonucleotide against monoamine oxidase-B (MAO-B) in rat brain striatal tissue. Cellular and Molecular Neurobiology 21: 53-64, 2001.

Kane, M.D., Jatkoe, T.A., Stumpf, C.R., Lu, J., Thomas, J.D, and Madore, S.J. Assessment of the sensitivity and specificity of oligonucleotide microarrays. Nucleic Acids Research 28: 4552-4557, 2000.

Kane, M.D., Lipinski, W.J., Callahan, M.J., Bian, F., Durham, R.A., Schwarz, R.D. Roher, A.E. and Walker, L.C. Evidence for seeding of beta-amyloid by intracerebral infusion of Alzheimer brain extracts in beta-amyloid precursor protein-transgenic mice. Journal of Neuroscience 20: 3606-3611, 2000.

9Kane, M.D., Schwarz, R.D., St. Pierre, L., Watson M.D., Emmerling, M.R., Boxer, P.A., and Walker, G.K. Inhibitors of V-type ATPases, bafilomycin A1 and concanamycin A, protect against beta-amyloid-mediated effects on 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. Journal of Neurochemistry 72: 1939-1947, 1999.

Yan, S.D., Stern, D., Kane, M., Kuo, Y-M., Lampert, H.C., and Roher, A.E. RAGE-Abeta?interactions in the pathophysiology of Alzheimer disease. Restorative Neurology and Neuroscience 12: 167-173, 1998.

Kane, M.D., Vanden Heuvel, J.P., Isom, G.E., and Schwarz, R.D. Differential expression of group I metabotropic glutamate receptors (mGluRs) in PC12 cells: role of nerve growth factor and ras. Neuroscience Letters 252: 1-4, 1998.

Kane, M.D., Yang, C.W., Gunasekar, G. and Isom, G.E. Trimethyltin stimulates protein kinase C translocation through receptor-mediated phospholipase C activation in PC12 cells. Journal of Neurochemistry 70: 509-514, 1998.

Pavlakovic, G., Kane, M.D., Eyer, C.L., Kanthasamy, A., and Isom, G.E. Activation of protein kinase C by trimethyltin: relevance to neurotoxicity. Journal of Neurochemistry 65: 2338-2343, 1995.

Sun, P., Borowitz, J.L., Kanthasamy, A.G., Kane, M. D., Gunasekar, P.G., and Isom, G.E. Antagonism of cyanide toxicity by isosorbide dinitrate: possible role of nitric oxide. Toxicology 104: 105-111, 1995

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