Tony Hazbun

Tony Hazbun Profile Picture

Associate Professor of Med Chem and Mol Pharm
Ph.D., 1997 - University of Notre Dame Postdoc - Howard Hughes Medical Institute, University of Washington, (Stanley Fields)

Contact Info:

Training Group(s):
Cancer Biology
Computational and Systems Biology

Active Mentor - currently hosting PULSe students for laboratory rotations and recruiting PULSe students into the laboratory; serves on preliminary exam committees

Current Research Interests:

Functional genomic information is being generated at an enormous pace and is helping to describe the function of the 6000 genes in the Bakers Yeast, Saccharomyces cerevisiae. However, this information is far from saturating since we still do not know the function of at least 30% of yeast genes. This lack of saturation is further evident when we look at specific biological processes. My research program develops and uses genome-wide methods in a targeted manner so that we can attempt to saturate the information from these methods for specific biological processes.

I am particularly interested in post-translational modifications and how they affect downstream signaling events such as protein-protein interactions. The lab uses a combination of genome-wide methods and single protein experiments to enable the elucidation of the role of these modifications in two biological processes: mitosis and chromatin.

Yeast is an excellent model for understanding chromosomal instability. It has simple chromosome segregation machinery relative to humans yet it is still intricate enough to provide a major modeling challenge from a systems biology view. Although there are some key differences with humans, the chromosome segregation machinery that is relatively well-conserved on a protein function and structural level. Functional genomic methods applied to yeast provide an excellent route toward obtaining a comprehensive description of this mitotically-linked machinery. Mitotic proteins are a rich source of potential targets for anti-cancer therapies and information from yeast will help in target selection. The identification of genetic interactions, protein-protein interactions, and post-translational modifications (PTMs) controlling mitosis and chromosome segregation in yeast should serve as a guide toward the analysis of and ultimately, intervention in, the abnormal mitotic pathways that propagate cancer cells.

Selected Publications:

1. Wong J, Nakajima Y, Westermann S, Shang C, Goodner C, Houshmand P, Chan C, Fields S, Drubin DG, Barnes G, Hazbun T. A protein interaction map of the mitotic spindle. Mol Biol Cell 18:3800-9, 2007

2. Jin F, Avramova L, Huang J, Hazbun T. A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nat Methods 4(5):405-7, 2007

3. Montpetit B, Hazbun TR, Fields S, Hieter P. Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and proper anaphase spindle elongation. J Cell Biol 174(5):653-63, 2006

4. Jin F, Hazbun T, Michaud GA, Salcius M, Predki PF, Fields S, Huang J. A pooling-deconvolution strategy for biological network elucidation. Nat Methods 3(3):183-9, 2006

5. Gingras AC, Caballero M, Zarske M, Sanchez A, Hazbun TR, Fields S, Sonenberg N, Hafen E, Raught B, Aebersold R. A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity. Mol Cell Proteomics 4(11): 1725-40, 2005

6. Guo D*, Hazbun TR*, Xu X-J, Fields S, Kuo M-H. A Tethered Catalysis Two-Hybrid System to Identify Protein-Protein Interactions Requiring Post-Translational Modifications. Nature Biotech Jul;22(7):888-92, 2004* Equal contributions

7. Hazbun TR, Malmstrom L, Anderson S, Graczyk BJ, Fox B, Riffle M, Sundin BA, Aranda J.D, McDonald WH, Chiu C-H, Snydsman BE, Bradley P, Muller EGD, Fields S, Baker D, Yates III, JR, Davis TN. Assigning Function to Yeast Proteins by Integration of Technologies. Mol Cell 12 (6):1353-65, 2003

8. Shang C, Hazbun TR, Cheeseman IM, Aranda J, Fields S, Drubin DG, Barnes G. Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1. Mol Biol Cell 14(8):3342-55, 2003

9. Hazbun TR, Fields S. A genome-wide screen for site-specific DNA-binding proteins. Mol Cell Proteomics 2002 1(7):538-43

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