Interdisciplinary Life Science - PULSe Great research is a matter of choice

Shelley Claridge

Shelley Claridge Profile Picture

Assistant Professor-Analytical Chemistry
Ph.D. - 1997 - University of California-Berkeley

Contact Info:

Training Group(s):
Membrane Biology

Current Research Interests:

Imaging structures of molecules, materials, and interfaces at length scales from 0.1–10 nm is a ubiquitous challenge in nanoscience. This size regime encompasses critical objectives – structures of transmembrane proteins that are the targets of half of all commercial pharmaceuticals, as well as organic–inorganic interfaces between nanocrystals and ligands that are a primary determinant of nanoscale device performance. However, studying heterogeneous organic and biological structures at these length scales is difficult due to the weak signals produced from most organic molecules in conventional imaging modalities.

A central theme in our group is the development of integrated imaging strategies that advance the limits of single-molecule structural analysis on the 0.1–10 nm scale, addressing challenges ranging from understanding protein structure to optimizing nanoscale device performance. These include:

development of custom scanning probe instrumentation and sample preparation strategies to enable imaging of samples not traditionally amenable to scanning probe characterization (including large proteins.) unconventional applications of bioanalytical techniques leveraging recent advances in the synthesis of layered materials such as graphene. integrating modeling and advanced imaging to address issues in single-molecule structural analysis of complex molecules, including finding solutions to inverse imaging problems (collaborations with science and engineering faculty in the Integrated Imaging Cluster at Purdue.) Since we evaluate organic and biological structures at interfaces, a second focus is on designing interfaces that stabilize important targets such as transmembrane proteins to enable characterization. One current strategy leverages polymerizable amphiphiles to create controlled hydrophilic–hydrophobic interfaces on appropriate length scales.Students in the group will develop expertise in a wide variety of nanoscale analysis techniques, both within the laboratory and at the Birck Nanotechnology Center. These include scanning probe microscopies, advanced surface analysis methods (such as polarization-modulated IR reflection absorption spectroscopy), and large-scale molecular modeling. Students may also develop new instrumentation to enable measurements of targets including membrane receptor proteins, in conjunction with the PuLSE membrane biology group at Purdue, or pursue modeling and image analysis projects in collaboration with faculty in the Integrated Imaging Cluster.

Selected Publications:

Claridge, S.A.; Liao, W.S.; Thomas, J.C.; Zhao, Y.; Cao, H.; Cheunkar, S.; Serino, A.C.; Andrews, A.M.; Weiss, P.S.,, From the Bottom Up: Molecular-Scale Control and Characterization at the Interface. Chemical Society Reviews 2013 , 42, 2725-2745.

Claridge, S.A.; Schwartz, J.J.; Weiss, P.S.,, Electrons, Photons, and Force: Quantitative Single-Molecule Measurements from Physics to Biology. ACS Nano 2011 , 5, 693-729.

Claridge, S.A.; Castleman, A.W.; Khanna, S.N.; Murray, C.B.; Sen, A.; Weiss, P.S.,, Cluster-Assembled Materials. ACS Nano 2009 , 3, 244-255.

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