Interdisciplinary Life Science - PULSe Great research is a matter of choice

Ryan Cabot

Ryan Cabot Profile Picture

Assistant Professor, Department of Animal Sciences
Ph.D., University of Missouri-Columbia, 2002

Contact Info:

Training Group(s):
Chromatin and Regulation of Gene Expression

Current Research Interests:

The research conducted in our laboratory is focused on learning how the mammalian embryo directs its development from a single cell to a complex group of differentiated tissues and ultimately a fully formed adult organism. We are particularly interested in understanding how in vitro manipulation procedures affect development of the pig embryo and how these effects can be circumvented to improve embryo quality and embryo viability. It is well-established that many of the in vitro manipulations performed on mammalian embryos (e.g., in vitro production and culture of embryos) are correlated with increased rates of developmental failure and altered gene expression in surviving live-born animals. One technique in particular, cloning by nuclear transfer, has given scientists the ability to produce live-born domestic animals that harbor targeted genetic modifications.

The benefits from increasing the quality of embryos produced following in vitro manipulation will have a large impact on several scientific fields. First, it will allow us to increase the reproductive efficiency of agriculturally important species. Secondly, understanding how to better handle mammalian embryos in vitro will benefit the biomedical community as a resource to generate animal models for human diseases. While the scientific community has gained tremendous insight into the mechanisms of many human diseases through the use of transgenic and knock-out mice, much more sophisticated models, perhaps using animals that are more 'physiologically relevant', may be found in genetically modified livestock species, like the pig.

Current projects in the lab are aimed at examining the how specific epigenetic modifications are mediated in the early embryo (e.g., histone methylation) and the mechanisms by which specific chromatin-interacting factors access the nucleus during development.

Selected Publications:

L Magnani and RA Cabot. 2008. In vitro and in vivo derived porcine embryos possess similar, but not identical, patterns of Oct4, Nanog and Sox2 mRNA expression during cleavage development. Molecular Reproduction and Development, in press.

L Magnani, K Lee, W Fodor, Z Machaty, RA Cabot. 2008. Developmental Capacity of Porcine Nuclear Transfer Embryos Correlate with Chromatin Remodeling mRNA Transcripts in Donor Cells. Molecular Reproduction and Development 75(5):766-776.

L Magnani and RA Cabot. 2007 Developmental arrest is induced when the chromatin remodeling protein SMARCA2 in overexpressed in cleavage stage porcine embryos. Molecular Reproduction and Development 74(10):1262-1267.

MF Sega, K Lee, Z Machaty and RA Cabot. 2007. Pronuclear stage porcine embryos do not possess a strict asymmetric distribution of lysine 9 dimethylation of histone H3 based solely on parental origin. Molecular Reproduction and Development 74(1), pages 2-7.

B Linder-Stuart, R Cabot, T Schwickert and RAW Rupp. 2004. The SNF2 domain protein family in higher vertebrates displays dynamic expression patterns in Xenopus laevis embryos. Gene 326, pages 59-66.

RA Cabot and RS Prather. 2003. Cleavage stage porcine embryos may have differing developmental requirements for karyopherins ?2 and ?3. Molecular Reproduction and Development 64 (3), pages 292-301.

RA Cabot, M Hannink, RS Prather. 2002. CRM1-mediated nuclear export is present during mammalian embryogenesis, but is not required for early cleavage. Biology of Reproduction 67 (3), pages 814-819.

K-W Park, L Lai, H-T Cheong, R Cabot, Q-Y Sun, G Wu, EB Rucker, D Durtschi, A Bonk, M Samuel, A Rieke, BN Day, CN Murphy, BD Carter, RS Prather. 2002. Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts. Biology of Reproduction 66 (4), pages 1001-1005.

Q-Y Sun, GM Wu, L Lai, A Bonk, R Cabot, K-W Park, BN Day, RS Prather, H Schatten. 2002. Regulation of mitogen-activated protein kinase phosphorylation, micortubule organization, chromatin behavior, and cell cycle progression by protein phosphatases during pig oocyte maturation and fertilization in vitro. Biology of Reproduction 66 (3), pages 580-588.

RA Cabot, B Kühholzer, AWS Chan, L Lai, K-W Park, K-Y Chong, G Schatten, CN Murphy, LR Abeydeera, BN Day, RS Prather. 2001. Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector. Animal Biotechnology 12 (2), pages 205-214.

NT Ruddock, Z Machaty, RA Cabot, RS Prather. 2001. Porcine oocyte activation: differing roles of calcium and pH. Molecular Reproduction and Development 59 (2), pages 227-234.

Q-Y Sun, GM Wu, L Lai, K-W Park, R Cabot, H-T Cheong, BN Day, RS Prather, H Schatten. Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro. 2001. Reproduction 122 (1), pages 155-163.

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