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Kimberly K. Buhman

Kimberly K. Buhman Profile Picture

Associate Professor, Nutrition Science
Ph.D., Nutritional Biochemistry, Purdue University, 1999

Contact Info:

Training Group(s):
Membrane Biology

Current Research Interests:

Research in Dr. Buhman's laboratory focuses on the process of intestinal lipid absorption and how it regulates whole animal energy balance. Current research in the laboratory addresses the following questions: 1) What is the role of triglyceride synthesis in absorptive cells of the intestine on whole animal energy balance? 2) What is the role of fatty acid catabolism in absorptive cells of the intestine on whole animal energy balance? 3) What is the role of triglyceride storage in lipid droplets during the process of intestinal lipid absorption?

Selected Publications:

1. Bowman, T.A., O’Keeffe, K., D’Aquila, T.D., Yan, Q. W., Griffin, J. D., Killion, E. A., Mashek, D. G., Buhman, K. K., Greenberg, A.S.†(2016) Acyl CoA synthetase 5 (ACLS5) Ablation in Mice Increases Energy Expenditure and Insulin Sensitivity and Delays Fat Absorption. Molecular Metabolism. In press.

2. D’Aquila, T.D., Sirohi, D., Grabowski, J.M., Hedrick, V.E., Paul, L.N., Greenberg, A.S., Kuhn, R.J., Buhman, K.K.†(2015) Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge. Plos One. 10 (5):e0126823 PMID 25992653

3. Kim, C.Y., Zhu, Y., Buhman, K.K., Kim K.H.†(2015) Dietary Selenate Attenuates Adiposity and Improves Insulin Sensitivity in High-Fat Diet-Induced Obese Mice. Journal of Functional Foods. 17: 33-42.

4. Lee, H.J., Zhang, W., Zhang, D., Yang, Y., Liu, B., Barker, E.L., Buhman, K.K., Slipchenko, L.V., Dai, M., Cheng, J.X.†(2015) Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol. Sci. Rep. 5: 7930. PMID: 25608867

5. Hung Y., Linden, M.A., Gordon, A., Rector, R.S., Buhman, K.K.†(2015) Endurance Exercise Training Programs Intestinal Lipid Metabolism in a Rat Model of Obesity and Type 2 Diabetes. Physiological Reports. 3:1 PMID: 2560212

6. Arentson, E., Koser, S., Ajuwon K., Buhman, K.K., Donkin, S.D.†(2014) Excess pregnancy weight gain and early energy-rich environment programs offspring for indications of metabolic. Nutrition Research. 34(3):241-9 PMID: 24655491

7. Uchida, A., Eustaquio, T., Leary, J.F., Cheng, J.X., and Buhman, K.K.†(2013) Intestinal acyl-CoA:diacylglycerol acyltransferase 2 overexpression enhances postprandial triglyceridemic response and exacerbates high fat diet-induced hepatic triglyceride storage. Biochim Biophys Acta – Molecular and Cell Biology of Lipids. 1831(8):1377-85.

8. Wilfling, F., Wang, H., Haas, J. T., Krahmer, N., Gould, T., Uchida, A., Cheng, J-X., Graham, M., Christiano, R., Frohlich, F., Liu, X., Buhman, K.K., Coleman, R.A., Bewersdorf, J., Farese, R.V. Jr., and Walther, T. C.†(2013) Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Developmental Cell. 24(4):384-99.

9. Uchida, A., Whitsitt, M.C., Eustaquio, T., Slipchenko, M.N., Leary, J.F., Cheng, J., and Buhman K.K.†(2012) Reduced triglyceride secretion in response to an acute dietary fat challenge in obese compared to lean mice. Frontiers in Fatty Acid and Lipid Physiology. 3: 1-10

10. Antalis, C., Uchida, A., Buhman, K.K., and Siddiqui, R. A.†(2011) Migration of MDA-MB-231 breast cancer cells depends on the availability of exogenous lipids and cholesterol esterification. Clinical & Experimental Metastasis. 28(8): 733-41.

11. Uchida, A., Slipchenko, M.N., Cheng, J.X., Buhman, K.K. (2011) Fenofibrate (FEN), a peroxisome proliferator activated receptor alpha (PPARa) agonist, decreases dietary fat absorption and alters triglyceride (TG) metabolism in enterocytes of mice. Biochim Biophys Acta 1811(3):170-6.

12. Lee, B., Zhu, J., Fast, A., Cheng, J-X., and Buhman K.K†. (2010) Intestine specific expression of acylCoA: diacylglycerol acyltransferease 1 (DGAT1) reverses the resistance to diet-induced hepatic steatosis and obesity in Dgat1-/- mice. Journal of Lipid Research. 51(7):1770-80.

13. Antalis, C.J., Arnold, T., Rasool, T., Lee, B-G., Buhman, K.K. and Siddiqui, R.A.†(2009) High ACAT1 expression in estrogen receptor negative basal-like breast cancer cells is associated with LDL-induced proliferation. Breast Cancer Res Treat 122(3):661-70.

14. Lee, B-G, Zhu, J, Wolins, N. Cheng J-X†, Buhman K.K.†(2009) Differential association of Adipophilin and TIP47 proteins with cytoplasmic lipid droplets in mouse enterocytes during dietary fat absorption. Biochim Biophys Acta. 1791(12):1173-80.

15. Zhu, J., Lee, B-G, Buhman K.K.†, Cheng J-X†. (2009) Direct Observation of a Dynamic Triglyceride Pool in Mouse Enterocytes during Dietary Fat Absorption by Coherent Anti-Stokes Raman Scattering Imaging. Journal of Lipid Research. 50(6):1080-9.

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