Bruce Applegate

PULSe Contributor - not currently hosting students for laboratory rotations or recruiting students in the laboratory

Current Research Interests:

My research interests include detection of viable foodborne pathogens using bateriophage, automated extraction of nucleic acids from various matrices, enumeration of microorganisms using quantitative PCR, the use of bioreporters in bioelectronics, metabolic engineering, detection of problematic microorganisms in industrial environments, construction of recombinant bacterial strains to rapidly evaluate antimicrobial products, and microbial ecology.

Patents

Applegate, B. M., Morgan, M., Perry, L., & Kothapalli, A. (2009). Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface. U.S. Patent No. Filed Utility Patent # 12/549,500.. Washington, D.C.: U.S. Patent and Trademark Office.

Applegate, B. M. (2005). Bioluminescent biosensor device. U.S. Patent No. 6,544,729. Washington, D.C.: U.S. Patent and Trademark Office.

Applegate, B. M., Morgan, M., Perry, L., & Kothapalli, A. (2009). Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface. U.S. Patent No. Filed Utility Patent # 12/549,500.. Washington, D.C.: U.S. Patent and Trademark Office. Applegate, B. M. (2005). Bioluminescent biosensor device. U.S. Patent No. 6,544,729. Washington, D.C.: U.S. Patent and Trademark Office.

Selected Publications:

Stratton, T., Garc, A., Applegate, B. M., & Youngblood, J. (2009). Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency.. Biomacromolecules, 10(5), 1173-1180.

Perry, L., SamMiguel, P., Minocha, U., Terekhov, A., Shroyer, M., Farris, L., . . . Applegate, B. M. (2009). Sequence analysis of Escherichia coli O157:H7 bacteriophage FV10 and identification of a phage encoded immunity protein that modifies the O157 antigen. Fems Microbiology Letters, 292(2), 182-186.

Kim, S., Schuler, B., Terekhov, A., Auer, J., Mauer, L., Perry, L., & Applegate, B. M. (2009). A bioluminescence-based assay for enumeration of lytic bacteriophage. Journal of Microbiological Methods, 79(1), 18-22. Applegate, B. M. (2008). Chlorine Dioxide Oxidation of Dihydronicotinamide Adenine Dinucleotide (NADH). Inorganic Chemistry, 47, 2205-2211.

Park, M., Banks, M. K., Applegate, B. M., & Webster, T. J. (2008). Influence of nanophase titania topography on bacterial attachment and metabolism.. International Journal of Nanomedicine, 4(3), 1-8. Applegate, B. M. (2008). Luminescence Techniques for the Detection of Bacterial Pathogens.. In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems (Vol. 1, pp. 213-230).

Habteselassie, M. Y., Bischoff, M. M., Blume, E., Applegate, B. M., Reuhs, B., Brouder, S., & Turco, R. (2008). Environmental controls on the fate of Escherichia coli in soil. Water, Air and Soil Pollution, 190, 143-155.

Applegate, B. M. (2005). BARD Israel US Agriculture Grants. Applegate, B. M. (2005). USDA/NRI. Sedgley, C., Nagel, A., Hall, D., & Applegate, B. M. (2005). Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro. International Endodontics Journal, 38(2), 97-104.