{"id":991,"date":"2022-09-27T00:15:30","date_gmt":"2022-09-27T00:15:30","guid":{"rendered":"https:\/\/www.purdue.edu\/fnr\/sites\/sepulveda\/?page_id=991"},"modified":"2026-09-21T19:34:58","modified_gmt":"2026-09-21T19:34:58","slug":"rana-pipiens-genome-sequencing","status":"publish","type":"page","link":"https:\/\/www.purdue.edu\/fnr\/sites\/sepulveda\/rana-pipiens-genome-sequencing\/","title":{"rendered":"Genome Sequencing"},"content":{"rendered":"
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Rana Pipiens Genome Sequencing<\/h1>\n\n\n\n

Principal Investigator(s):<\/strong> Dr. Marisol Sep\u00falveda, Dr. Amanda Pendleton and
Dr. Tyler Hoskins<\/p>\n<\/div>

\"Rana<\/figure><\/div>\n\n <\/div>\n<\/div>\n <\/div>\n<\/div>\n\n
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Researchers<\/h2>\n\n\n\n
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\"Amanda<\/figure>\n<\/div>\n\n\n

Amanda Pendleton<\/strong>
Post-doctoral Researcher<\/em>
<\/sup>Purdue University<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n

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\"Tyler<\/figure>\n<\/div>\n\n\n

Tyler Hoskins<\/strong>
Research Assistant Faculty<\/sup><\/em>
Purdue Forestry & Natural Resources<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n

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\"Dr.<\/figure>\n<\/div>\n\n\n

Marisol Sep\u00falveda<\/strong>
Professor and Associate Head of Research<\/sup><\/em>
Purdue Forestry & Natural Resources<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div><\/div>\n\n <\/div>\n<\/div><\/div>\n\n\n\n

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Research Objectives<\/h2>\n\n\n\n

A native to Indiana, the Northern USA, and Canada, the Northern leopard frog, Rana pipiens, has had historical, impactful roles in the study of endocrine disruption, cancer and drug discovery, conservation biology, and ecotoxicology. Coupling these continuing research avenues with R. pipiens along with the ease of laboratory rearing and achieving transitions from aquatic larval to terrestrial adult stages in a single season, R. pipiens serves as an excellent model system. However, no reference genome exists for this species, which limits its utility in multiple disciplines. Furthermore, there is a dire need for more amphibian genomes, as this large and diverse taxonomic group is among the least abundant in the list of available animal genomes.<\/p>\n\n\n\n

Our goal is to generate a high-quality draft genome assembly of the triploid R. pipiens using long and short-read sequencing technologies and a comprehensive gene call set using already sequenced transcriptomic resources.<\/p>\n\n\n\n