General Safety Overview

***All User Make-up Information - Complete the Missed All User meeting form to restore access to laboratory/cleanroom/bio-cleanroom and turn into BRK 1220***
  • General Glove Use
    • Blue Microflex general use - One glove rule when non-chemical/ biological use
    • Biological Safety Level 1 - Gray Gloves must be removed before leaving the laboratory
    • Biological Safety Level 2 - Black Gloves must be removed before leaving the laboratory
  • Two types of fume hoods - Acid/Base and Solvent
    • PPE requirements:
      • Disposable gloves with acid/solvent proof over them
      • Solvent hood requires lab coat and goggles
  • Acid/base smock and goggles or face shield if Acid Hood is used during dispensing from original containers. All material left in the hood must be labeled with the following information:
    • Students name - first and last
    • Major constituents of solution (please write out- example:
      hydrochloric acid do not use HCL
    • Current date
  • Make sure once any chemicals have been added to waste containers that the funnel has been removed and the container capped.
    Leave one/one and a half panes of hood face open at all times.
  • This will ensure air flow stays in the 80-125 LFM required to maintain adequate containment at the hood face.
  • Always leave hood sash down unless setting up equipment
  • Before using a hood note where the eyewash/ safety shower is and telephone
  • Waste Disposal
    • Acids and bases sink disposal with copious amounts of water
      • Heavy metal bases materials “Chromic Acid” must be containerized as hazardous waste also plating waste 
    • Solvents are placed in “white antifreeze containers”
      • Non-halogenated - methanol, isopropanol, acetone, toluene, ethyl acetate, Methyl ethyl ketone etc.
      • Halogenated - chlorobenzene, methylene chloride, 1,1,1 trichloroethane etc.
  • Empty Container Handling
    • Empty solvent container- Blow dry or until no liquid drips from container with nitrogen and place in red storage bin with safe for disposal label affixed and leave cap off.
    • Empty Acid containers - Triple rinse with water, blow dry until no ,liquid drips from container  with nitrogen, rinse and wipe exterior of bottle, affix safe disposal label and place in red storage bin with cap off.
  • HF First Aid - Plastic tub with lid. Emergency procedure inside.
    • Decontamination rinse is in small refrigerator in galley marked “FirstAid HF Decontamination Solution only! 0.13% Benzalkonium Chloride
    • Galley - Location of refrigerator for Benzalkonium Chloride, MSDS locations and spill carts and fire extinguishers. Emergency call 911
    • Spill Cart - designed for student use small spills < 1 gal
      • Use sorbent pad for acids/bases and solvents
      • Spill booms for material headed under the door
      • PPE for acids/bases
      • pH paper the unknown materials
      • > 1 gallon contact Staff 63333
      • Sign for posting on door
  • Samples that leave the laboratory - Use one glove method to transport samples to another laboratory
  • MSDS Information - Location on end of Galleys
    • MSDS manuals contain MSDSs by room #
    • Also available Chemical Hygiene Plan, Personal Protective Equipment Policy and LASER Policy
    • Ordering chemicals - require an electronic copy of the MSDS at the time order is being placed. If through another department please e-mail (  a copy for approval. Either ship direct to BNC or use campus mail. If using campus mail you will need to place your name and laboratory you are planning to store the material in. Material cannot be shipped to an office in BNC.
  • Handling of sharps
    • All sharps which include plastic pipette tips must be place in a sharps container
    • Syringes with needles shall not be recapped but placed directly into a sharps container
  • General Lab Information
    • Location of Hazard Assessment
    • Location of the staff contact list

Helpful Safety Links

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