Instruments

NEW! MoFlo Astrios High Speed Cell Sorter

This cell sorter contains four state of the art lasers that can detect up to 14 fluorescent parameters in a single sample and simultaneously sort 4 individual cell populations. Its high speed sorting rate allows researchers to acquire large numbers of viable cells from rare populations, providing a platform to tackle complex biological questions in ways that were not previously possible.

NEW! BD FACS Aria Fusion Cell Sorter

The BD FACS Aria Fusion is a  5-laser, 14-color, 16-parameter high-speed cell sorter with temperature control and housed in an integrated biosafety cabinet. Wavelength excitation choices include 405-nm, 488-nm, 561-nm, 633-nm and 355-nm (UV) lasers. With the advanced gel-coupled cuvette and octagon- and trigon-shaped detector arrays that maximize signal detection, the BD FACSAria III represents the state of the art in cell sorting. In addition, we will also do analysis-only experiments on the FACS Aria that require greater than four colors. 

BD FACS Aria III Cell Sorter

The BD FACS Aria III Cell Sorter is a 5-laser, 14-color, 16-parameter high-speed cell sorter with temperature control and housed within a biosafety cabinet for sorting of biohazardous samples. Wavelength excitation choices now include 405-nm, 488-nm, 561-nm, 633-nm and 355-nm (UV) lasers.  Gel-coupled cuvette and octagon- and trigon-shaped detector arrays  maximize signal detection. We will do analysis-only experiments on this instrument that require greater then four colors.

BD Fortessa Cell Analyzer

The BD Fortessa LSR flow cytometry cell analyzer has the same 5-laser setup as the ARIA cell sorters, with 18-color, 20-parameter detection and a high throughput microwell sample uptake system. This cytometer is available for operation by students and staff. This instrument is used for immunophenotyping, phosphoprotein analysis, DNA analysis, cell cycle and functional assays.

Fluidigm C1 System

Fluidigm's C1 system is designed to isolate, process, and prepare individual cells for genome and transcriptome analysis. The instrument is used to sort cells in liquid suspension into 96 separate chambers to prepare them for DNA or RNA extraction. In partnership with the Purdue Genomics Core Facility, researchers apply NGS to identify changes in the genome or the gene expression patterns that affect cellular events and metabolic processes.