
Instruments
BD FACS Aria SORP and BD FACS Aria Fusion Cell Sorters
The BD FACS Aria SORP and FACS Aria Fusion Cell Sorters are 5-laser, 14-color, 16-parameter high-speed cell sorters with temperature control, that are housed in integrated biosafety cabinets for sterile sorting of biohazardous samples. Wavelength excitation choices include 405-nm, 488-nm, 561-nm, 633-nm and 355-nm (UV) lasers.
BD Fortessa Cell Analyzer
The BD Fortessa LSR flow cytometry cell analyzer has the same 5-laser setup as the ARIA cell sorters, with 18-color, 20-parameter detection and a high throughput microwell sample uptake system. This cytometer is available for operation by students and staff. This instrument is used for immunophenotyping, phosphoprotein analysis, DNA analysis, cell cycle and functional assays.
Attune NxT Flow Cytometer
This benchtop flow cytometer has 4-laser excitation, 14-parameter detection capabilities, automated multiwell sampling and automated report generation. Wavelength excitation choices include 405-nm, 488-nm, 561-nm, and 627-nm. This cytometer is available for operation by students and staff. This instrument is used for nanovesicle detection, immunophenotyping, phosphoprotein analysis, DNA analysis, cell cycle and functional assays.
10X Genomics Chromium DropSeq
The Chromium system is designed to isolate, process and prepare individual cells for genome and transcriptome analysis. The instrument is used to sort cells in liquid suspension into 96 separate chambers to prepare them for DNA or RNA extraction. Researchers apply NGS to identify changes in the genome or the gene expression patterns that affect cellular events and metabolic processes.
Contact
Jill Hutchcroft
Flow Cytometry Facility Director
Phone: (765) 586-1130
Email: hutchcroft@purdue.edu