Mass Spectrometry


Mass spectrometry is an essential analytical technique for the identification and structure elucidation of the wide variety of compounds that are important to PCCR members’ research projects. These projects include studies on the design and synthesis of therapeutic agents for cancer treatment, the creation of diagnostic agents and the investigation of basic cellular processes involved in cancer cell biology. Of additional importance is the ability of mass spectrometry to sort out reaction products to facilitate increased yield and understand where a reaction might be going wrong when the expected product is not obtained.

As part of the campus-wide Mass Spectrometry Center, the Mass Spectrometry Shared Resource (MS-SR) provides PCCR members access to all of the facility’s mass spectrometers. This includes support for both routine analyses and collaborative analytical mass spectrometric capabilities (including problem-solving by mass spectrometry). Taking advantage of the MS-SR staff’s experience, in addition to routine mass spectrometry analyses, considerable effort has been made to make each of the ionization techniques a center of excellence for solving the difficult mass spectrometry research problems associated with PCCR research projects.

The MS-SR currently has eight mass spectrometers available to PCCR members. This includes:

  • Electrospray Ionization (ASI), Matrix-Assisted Laser Desorption Ionization (MALDI) and Atmospheric Pressure Chemical Ionization (APCI) for the analysis of nonvolatile components.
  • Electron Impact (EI) and Chemical Ionization (CI) for volatile components.
  • Inductively Coupled Argon Plasma Ionization (ICP) for multielement analysis.
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Center for Drug Discovery — DRUG, Room B059

  • Applied Biosystems Voyager DE Pro: MALDI-ToF analysis of intact, nonvolatile compounds up to 100,000 m/z
  • Applied Biosystems/MDS Sciex 4800: MALDI-ToF/ToF instrument with MS/MS capability for compound characterization and identification
  • Agilent 5975C GCMS: Quadrupole mass spectrometer with gas chromatograph, ionization by EI, CI and NCI for volatile compounds up to 1000 m/z
  • Agilent 6550 LC-MS/MS: Q-ToF mass spectrometer with nanoflow LC and ion funnel technology for increased sensitivity and reduced limit of detection. MS/MS and MRM capabilities along with high resolution (50,000) and accurate mass (< 5 ppm) detection. Mass range up to 10,000 m/z
  • Advion ExpressION CMS: Walkup instrument located in DRUG 140A. Only available to users with card-swipe access to DRUG. Single quadrupole instrument with electrospray ionization. Also capable of direct analysis of TLC plates. APCI ionization available upon request. For compounds up to 2000 m/z. To access this instrument, please submit a training request through iLab.

Wetherill Chemistry Building (WTHR, Rooms 144, 150, 151)

  • Thermo Fisher Element 2 ICP-MS: High resolution double focusing magnetic sector instrument for elemental analysis, including trace levels. Equipped with an autosampler and an Aridus II desolvating nebulizer for enhanced sensitivity and reduced interferences.
  • HP 5989 GCMS: Quadrupole mass spectrometer with gas chromatograph, ionization by EI and CI for volatile compounds up to 1000 m/z. Equipped with a direct insertion probe as an alternative means of sample introduction.
  • Agilent 6320 LC-MS/MS: Ion trap instrument equipped with LC along with ionization by ESI or APCI. MSn capability for compound characterization. For nonvolatile compounds up to 50,000 m/z.
  • Thermo Fisher Orbitrap Classic FTMS: High resolution (100,000) and accurate mass (<5 ppm) measurements with ionization by ESI or APCI. MS/MS for compound characterization.

Services and Pricing

Prices are per-sample estimates representative of typical or average charges.

Service Internal Clients Service Service
Agilent 6550 QTOF $32 $21.67 $60.33
Agilent 5975C GC/MS $27 $16.67 $55.33
HP 5989 GC/MS $27 $16.67 $48.67
LTQ Orbitrap Classic $27 $16.67 $48.67
MALDI 4800 ToF/ToF $27 $16.67 $51.67
MALDI Voyager De Pro $27 $16.67 $50.33
Element 2 ICP-MS $13.50 $8.33 $22.67
Agilent 6320 Ion Trap $27 $16.67 $45
6550-LCMS $64 $43.33 $120.67
6320-LCMS $54 $33.33 $90

Contact Information and Locations

Ryan T. Hilger
Interim Director, Campus-wide Mass Spectrometry Center

Arlene Rothwell
Chemistry (Wetherill Laboratory of Chemistry, Rooms 145/151)

Connie Bonham
Drug Discovery, Room B059
765-494-1569 (office)
765-494-0707 (lab)