Shared Resource and Affiliated Facility Projects

Funding Opportunity Number

PCCR-SR-FY2020-02

Funding Opportunity Title

SHARED RESOURCE and AFFILIATED FACILITY PROJECTS 

Key Dates

Events Cycle 1 Cycle 2
Announcement August 27, 2019 August 27, 2019
Due October 1, 2019 March 9, 2020

Administrative Contact

John Fry (johnfry@purdue.edu)

Funding Opportunity Description

The Executive Committee of the Purdue Center for Cancer Research (PCCR) would like to allocate funds to support Cancer-Related projects that will utilize the Center's Transgenic and Genome Editing (TGEF), Biological Evaluation (BE), Flow Cytometry (FC), DNA Sequencing (SEQ), Purdue Cryo-EM Facility and The Purdue Bioscience Imaging Facility (BIF).  There are also funds to help with cell line testing associated with in vivo experimentation.  These funds will be given to contribute to the costs of acquiring new and important data in support of grant or manuscript submission or revision. Funding for this opportunity is supported by generous donations by the Indiana Elks, gifts and other endowed funds to the Center for Cancer Research.

CLICK HERE to submit your electronic application by 5 pm on the cycle due date. No exceptions. The electronic submission system will not allow applications beyond the deadline. Once here you can enter your Purdue credentials.  If somehow the password entry does not allow access, please click on "more choices."  Click "Use a different account" and enter "onepurdue\" in front of the user name and re-type your password.  If there are further login problems, please email the administrative contact listed above.

Qualifications/Restrictions

Applicants must be members of the Purdue University Center for Cancer Research. Only one application indicating the use of one shared resource per center member is allowed. No exceptions.

Cost share

This announcement does not require cost sharing.

Funds Available and Anticipated Number of Awards

The number of awards is contingent upon PCCR endowments, gifts and other funds and the submission of meritorious applications.

Award Budget

See the individual descriptions that follow. Each shared resource has separate budget guidelines and the suggested budget numbers are flexible if sufficient justification is provided (not included in two-page limit).

Award Project Period

See the individual descriptions that follow, however it is expected that work will commence within less than two (2) months of the date of award.  A project period of no more than six (6) months will be assigned to projects.

Award Restrictions

This award may only be used to pay for services in the indicated facilities in this announcement.

Award Requirements

Acceptance of an award will indicate your willingness to serve on the Center’s Pilot Grants Review Committee and to possibly represent the Center at the annual Indiana Elks Convention.  The awardee MUST acknowledge the PCCR. Suggested publication acknowledgement:

“The authors gratefully acknowledge the <insert shared resource name> and support from the Purdue University Center for Cancer Research, P30CA023168”

No Cost Extension (NCE)

No cost extensions are discouraged.  An exception might be considered on a case-by-case basis. Requests must be made to the administration of the PCCR.   Any problems associated with facility processing of the project should be communicated before the end of the award period to the PCCR.

Regulatory

Please note the award is contingent upon obtaining all of the necessary regulatory approvals (e.g., IRB, IACUC, PACUC) or a Memorandum of Understanding (MOU) pertaining to the work that has pending approvals.

Reporting

Responding to periodic request of progress is required. Triage of future applications may occur if reporting requests are ignored.

Proposal Guidelines

  1. Proposal Length: The proposal should be single-spaced and a maximum of two pages in length (Arial, 11pt) with no less than 0.5 inch margins.
  2. Required Proposal Structure:
    1. Title, Applicant(s) name, Applicant’s program and proposed facility
    2. Brief Significance and Background
    3. Approach: Please see the facility descriptions for specific information.
    4. Timeline and Specific Outcomes: Describe the expected outcomes and evidence that it will be ready to start within two (2) months.  The applicant must specify the following:
      • a peer-reviewed grant submission that will incorporate the data acquired from the shared resource, and/or
      • contributions of the shared resource data to a new or re-submission peer-reviewed publication.
      • the granting agency or journal should be clearly identified as well as the timeline given for the submission. Preference will be given to proposals that have been scored but not funded and are in need of additional supporting data.
  3. Interaction with the shared resource staff to plan the budget and design of experiments is required.
  4. For the electronic submission, the items to upload should include:
    • Two page proposal
    • Cited literature (not in the page limit)
    • NIH Biosketch
    • Current and Pending Funding
    • Letters of Support (if applicable)  

Review Criteria

  1. The primary review criteria will be:
    1. The scientific impact of the shared resources on the project and the usage in securing additional funding of the project or in getting a manuscript accepted
    2. The feasibility of completing the project within 6 months. No cost extensions are discouraged.  An exception might may be considered on a case-by-case basis. Requests must be made to the administration of the PCCR.  Any problems associated with facility processing of the project should be communicated before the end of the award period to the PCCR.
  2. The appropriateness of the budget requested for supplies, where applicable.
  3. Productivity from previous internal awards will be assessed and weighted appropriately.
  4. Reviewers could consist of senior/program leaders, for scientific impact, and shared resource directors for project feasibility.

PCCR SHARED RESOURCES

Transgenic and Genome Editing (TGEF)

  1. If producing a gene-edited mouse: a description of the proposed mutation and evidence that the gene fragment and/or CRISPR guides are  available or will be generated shortly.
  2. A statement indicating how a new gene-edited mouse model and/or other data produced will advance cancer research.
  3. Up to $3,500 facility credit can be given. A budget of up to $500 may also be requested to prepare samples for analysis in addition to the facility credit. An explanation of the need for additional funds to prepare samples will be required.
  4. CRISPR-Cas9 Design and Build
    1. Funds for projects will be used to support usage of the TGEF facility’s new and state-of-the-art CRISPR-Cas9 services, provided in conjunction with a vendor (or internally at Purdue), to build an organism (i.e. mouse) with the TGEF. The amount to support the project will not exceed $5,000 (Note: funds for invoices will be direct pay by the center business office).
  5. Applicants are encouraged to contact Dr. Stephen Konieczny, Scientific Director of the TGEF (email: sfk@purdue.edu) to discuss technical aspects and feasibility of the potential projects and Judy Hallett (email: halletje@purdue.edu) to help with CRISPR vendor selection, if applicable.

Flow Cytometry (FC)

  1. Funds for projects will be used to support usage of the facility equipment and especially those projects that make use of the new and state-of-the-art flow cytometry/sequencing instruments located in this facility.
  2. A description of the proposed project.
  3. A statement indicating how the data produced will advance cancer research and your project.
  4. Up to $2,500 facility credit can be given. A budget of up to $500 in supplies may also be requested to prepare samples for analysis in addition to the facility credit. An explanation of the need for additional funds to prepare samples will be required.
  5. Applicants are encouraged to contact Dr. Jill Hutchcroft, Facility Director of Flow Cytometry (email: hutchcroft@purdue.edu) to discuss technical aspects and feasibility of the potential projects.

Biological Evaluation (BE)

  1. A description of the proposed in vitro and/or in vivo project
  2. A statement indicating how the data produced will advance cancer research and your project.
  3. Up to $5,000 facility credit can be given. A budget of up to $500 may also be requested to prepare samples for analysis in addition to the facility credit.  In addition, a budget up to $500 may also be requested for facility credit within the Purdue Bioscience Imaging Facility for use with the described project.  An explanation of the need for additional funds to prepare or image samples will be required.
  4. Applicants are encouraged to contact Dr. Bennett Elzey, Scientific Director of the BE (email: belzey@purdue.edu) to discuss technical aspects and feasibility of the potential projects.

DNA Sequencing and DNA sequencing in combination with Bioinformatics Analysis (SEQ)

  1. Funds for projects will be used to support usage of the Hi-Seq and Mi-Seq state-of-the-art facility equipment.
  2. A description of the proposed project.
  3. A statement indicating how the data produced will advance cancer research and your project.
  4. Up to $10,000 facility credit can be given. A budget of up to $250 in supplies may also be requested to prepare samples for analysis in addition to the facility credit indicated. Please indicate whether this project may include resulting analysis to be performed in the Collaborative Core for Cancer Bioinformatics.  An explanation of the need for additional funds to prepare samples will be required.
  5. Applicants are encouraged to contact Dr. Phillip SanMiguel, Scientific Director of DNA Sequencing (email: pmiguel@purdue.edu) to discuss design/technical aspects and feasibility of the potential projects.

AFFILIATED FACILITY SUPPORT:

The Purdue Bioscience Imaging Facility (BIF)

  1. A description of the proposed in vitro and/or in vivo project
  2. A statement indicating how the data produced will advance cancer research and your project.
  3. Up to $2500 facility credit can be given.
  4. Applicants are encouraged to contact Andy Schaber (email: schaber@purdue.edu) to discuss facility use and discuss technical aspects and feasibility of the potential projects.

The Purdue Cryo-EM Facility (CEM)

  1. Single particle analysis on cancer-relevant targets are being sought to advance the scientific impact of structural biology in cancer at Purdue. Cancer-relevant targets would include proteins and enzymes and their complexes with other proteins, DNA/RNA and small molecule drugs.  Viruses are excluded.
  2. A description of the proposed project and a statement indicating how the data produced will advance cancer research and your project.
  3. Up to $5,000 in facility credit can be given and can include up to $250 in supplies for grid preparation.
  4. Applicants are encouraged to contact Dr. Wen Jiang, Scientific Director (email: jiang12@purdue.edu), to discuss facility use, a budget and a plan for executing the project.

OTHER SUPPORT AVAILABLE:

In December 2018, PACUC approved a new policy requiring biological agents (tumors, tissues, cell lines, body fluids, antibody preparations, and hybridomas) which are from rodents, or which have been passaged in rodents, to be evaluated for contamination prior to experimental use in vivo.  PACUC is now enforcing this policy, and as such, they are partnering with IDEXX to provide this service.  To help defray costs associated with this requirement, the PCCR will support up to $500 total (not per cell line) towards costs associated with testing.  If multiple cell lines are required, it will not alter the maximum amount of $500 in a given fiscal year.  The cancer-relevance must be strong in order for this support to be awarded.

Announcement History And Updates

Funding Opportunity Number Summary of Change
PCCR-SR-FY2020-01 Initiation of Announcement
PCCR-SR-FY2020-02 Edits to TGEF