February 4, 2004
Purdue researchers create device that detects mass of a single virus particle
WEST LAFAYETTE, Ind. Researchers at Purdue University have developed a miniature device sensitive enough to detect a single virus particle, an advancement that could have many applications, including environmental-health monitoring and homeland security.
The device is a tiny "cantilever," a diving board-like beam of silicon that naturally vibrates at a specific frequency. When a virus particle weighing about one-trillionth as much as a grain of rice lands on the cantilever, it vibrates at a different frequency, which was measured by the Purdue researchers.
"Because this cantilever is very small, it is extremely sensitive to added mass, such as the addition of even a single virus particle," said Rashid Bashir, an associate professor of electrical and computer engineering and biomedical engineering.
Findings are detailed in a paper to appear next month in Applied Physics Letters, a journal published by the American Institute of Physics. The paper, which is likely to appear in the weekly journal's March 8 issue, was written by doctoral student Amit Gupta, senior research scientist Demir Akin and Bashir, all in Purdue's School of Electrical and Computer Engineering.
The work, funded by the National Institutes of Health, is aimed at developing advanced sensors capable of detecting airborne viruses, bacteria and other contaminants. Such sensors will have applications in areas including environmental-health monitoring in hospitals and homeland security.
"This work is particularly important because it demonstrates the sensitivity to detect a single virus particle," Gupta said. "Also, the device can allow us to detect whole, intact virus particles in real time. Currently available biosensing systems for deadly agents require that the DNA first be extracted from the agents, and then it is the DNA that is detected."
The next step will be to coat a cantilever with the antibodies for a specific virus, meaning only those virus particles would stick to the device. Coating the cantilevers with antibodies that attract certain viruses could make it possible to create detectors sensitive to specific pathogens.
"The long-term goal is to make a device that measures the capture of particles in real time as air flows over a detector," Bashir said.
Scientists are striving to create "lab-on-a-chip" technologies in which miniature sensors perform essentially the same functions now requiring bulky laboratory equipment, saving time, energy and materials.
Thousands of the cantilevers can be fabricated on a 1-square-centimeter chip, Akin said.
The Purdue researchers used the device to detect a particle of the vaccinia virus, which is a member of the Poxviridae family and forms the basis for the smallpox vaccine.
The cantilever is about one micron wide or about one-hundredth the width of a human hair 4 microns long and 30 nanometers thick. A nanometer is a billionth of a meter, or roughly the length of 10 hydrogen atoms strung together.
"This cantilever mechanically resonates at a natural frequency, just like anything that vibrates has a natural frequency," Bashir said. "What we do is measure the natural frequency of the cantilever, which is a function of its mass. As you increase the mass, the frequency decreases. And the way to increase the sensitivity is to make that starting mass very, very small."
A single vaccinia virus particle weighs about 9 femtograms, or quadrillionths of a gram.
"So, if a grain of rice weighs a couple of milligrams, one of these virus particles weighs about one-trillionth as much," Bashir said.
Because the cantilevers are mechanical parts measured primarily on the scale of microns, or millionths of a meter, they are called "micromechanical devices."
The researchers created the cantilever using the same technology used by the semiconductor industry to etch circuits in electronic chips. Silicon is deposited as a blanket onto the surface of a wafer and then formed into patterns during numerous steps, including chemical etching. In this case, a cantilever is formed instead of a circuit.
In addition to funding from NIH, facilities in Purdue's Birck Nanotechnology Center were used to carry out the experiments.
Writer: Emil Venere, (765) 494-4709, firstname.lastname@example.org
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Amit Gupta, firstname.lastname@example.org
Demir Akin, email@example.com
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Single virus particle mass detection
Laboratory of Integrated Biomedical Micro/Nanotechnology and Applications, Birck Nanotechnology Center, School of Electrical and Computer Engineering, Department of Biomedical Engineering, Purdue University
In this letter, we present the microfabrication and application of arrays of silicon cantilever beams as nanomechanical resonant sensors to detect the mass of individual virus particles. The dimensions of the fabricated cantilever beams were in the range of 4-5 um in length, 1-2 um in width and 20-30 nm in thickness. The virus particles we used in the study were vaccinia virus, which is a member of the Poxviridae family and forms the basis of the smallpox vaccine. The frequency spectra of the cantilever beams, due to thermal and ambient noise, were measured using a laser Doppler vibrometer under ambient conditions. The change in resonant frequency as a function of the virus particle mass binding on the cantilever beam surface forms the basis of the detection scheme. We have demonstrated the detection of a single vaccinia virus particle with an average mass of 9.5 fg. These devices can be very useful as components of biosensors for the detection of airborne virus particles.