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To evaluate the health risks, work processes and hazardous materials related to those persons exposed to animals in their work and the environment in which it is conducted, whether in teaching, testing or research at Purdue. This includes any person working with non-fixed animal tissue, blood or body fluids within a laboratory or work environment. It is also to inform the individual of the risk and ways to mitigate those risks.
The AEOHP is an important part of Purdue University’s institutional animal care program. This program, operated through the Vice President for Research, Purdue Animal Care and Use Committee (PACUC) and the Office of the Vice President for Physical Facilities, is designed to protect both Purdue personnel and vertebrate animals. The following information is an introduction to the AEOHP and provides information and training to individuals with animal exposure concerning the risks associated with that exposure. The requirements of the AEOHP are based on guidelines in the NIH Guide for the Care and Use of Laboratory Animals.
Purdue’s animal care and use program and its animal facilities are accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC-I). AAALAC-I is a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs.
The AEOHP is an important component of Purdue’s animal care program and is based upon risk assessment and risk reduction. The AEOHP applies to Purdue University’s West Lafayette Campus, regional campuses, and research and teaching farms.
Vertebrate animals have the potential to cause injury, transmit zoonotic disease, and/or cause allergic reaction to those who have contact. This can be either direct contact from handling an animal or from being in close proximity, i.e., working or passing through an animal housing room. The program applies to personnel (staff members or qualified students) who in the conduct of their work in research, testing and teaching have direct contact with vertebrate animals including their tissues, body fluids, wastes and/or indirect animal aerosol exposure. Personnel who have these types of exposures need to be provided with the appropriate awareness training. Understanding routes of disease transmission, disease or allergy signs and symptoms, personal protective equipment, waste handling, and emergency contacts is very important. The AEOHP strives to provide a safe working environment for employees who work in areas that house or use vertebrate animals.
The requirements of this program are based on guidelines found in the Public Health Service (PHS) policy and the Guide for the Care and Use of Laboratory Animals as well as maintaining AAALAC-I accreditation. The PHS Policy on Humane Care and Use of Laboratory Animal requires institutions that receive federal funds to provide occupational healthcare services to employees who work in vertebrate animal facilities or have frequent contact with animals. This same policy requires animal care and use programs to follow the recommendations of the Guide for the Care and Use of Laboratory Animals. This guide not only provides guidelines and references for the welfare of animals, but also recommends an Occupational Health and Safety Program for those working in animal facilities and having exposure to animals in other areas.
In many cases a preventative annual physical examination is necessary for personnel handling animals. To accomplish this, Purdue University has the AEOHP that administers this duty. The first step is to inform personnel of this program.
The AEOHP is not mandatory, individuals have the option of declining this program if they chose to do so; however, the individual will have received a risk assessment of their position in regards to working with animals.
Purdue is committed to maintaining AAALAC-I accreditation which includes specific guidelines for occupational health and safety. The Public Health Service (PHS) Policy on Humane Care and Use of Laboratory Animal requires institutions that receive federal funds to provide occupational healthcare services to employees who work in research, teaching or testing animal facilities or have frequent contact with animals. This same policy requires Institutional Animal Care and Use Committee programs to follow the recommendations of the Guide for the Care and Use of Laboratory Animals. This guide not only provides guidelines and references for the welfare of research animals, but also recommends an Occupational Health and Safety Program for those working in animal facilities and having exposure to animals.
Participation in the AEOHP is provided at no cost to the individual participant.
If after reading this material, you choose to participate in the AEOHP, please contact the OHS Specialist, Carol Oteham (email@example.com, or 61334)
Employees who work with animals must complete animal specific training modules for the animals(s) they work with. The training is currently provided by the Laboratory Animal Training Association. In the future, the Collaborative Institutional Training Initiative (CITI) will host this training. Please contact the PACUC (firstname.lastname@example.org) office for additional assistance on completing these training modules.
Employees may need to complete additional safety training deemed necessary by the supervisor, PACUC/LAP or OHSS based on risk assessment and their work assignments. Courses include:
Employees can learn more about the training listed above by clicking on the links and completing the form or registering with the appropriate contact person for that area.
In conjunction with PACUC’s semi-annual inspection process, the OHSS conducts comprehensive safety and health audits of University animal facilities and laboratories. The goal of the audits is to identify risk and safety hazards and to have them corrected in a timely manner.
The personal protective equipment (PPE) requirements of the Occupational Safety andHealth Administration (OSHA) regulations, 29 CFR 1910.132 applies to the use of personal protective equipment at the West Lafayette Campus, regional campuses, and research and teaching farms.
Personal protective equipment such as protective clothing, respiratory devices (respirators), shields, and barriers shall be used to protect against chemical, radiological, biological, or mechanical hazards and irritants capable of causing injury or impairment through absorption, inhalation, or physical contact. Purdue University policy is that personal protective equipment be provided, used, and maintained in a sanitary and reliable condition.
PPE is the last barrier between you and the hazard. Working with vertebrate animals exposes personnel to risks including injury from bites and scratches and contracting disease from the animals. Personnel also pose a threat to the health of animals on campus, as humans carry a number of infectious organisms that can be harmful to animals. In addition, allergies to animals are rapidly becoming one of the most common conditions adversely affecting personnel involved with the care and use of animals. Minimize the risk of development of animal allergies or zoonotic disease by working in well-ventilated areas, using good hygiene practices (proper hand washing technique), using personal protective equipment (PPE) such as gloves, and wearing N-95 rated dust masks, and laboratory coats or coveralls.
To safeguard both personnel and animals, all personnel with direct animal contact must wear the PPE provided by the supervisor or manager.
PPE shall comply with appropriate American National Standard Institute (ANSI) standards, when standards exist.
After performing a hazard assessment and determining that risk and hazards are present, or likely to be present, the OHSS and or supervisor shall do the following:
After a hazard assessment has been performed and hazards identified that require PPE, the employee shall do the following:
Note: Areas requiring the use of eye protection should have a sign posted at the entrance. The sign should indicate “EYE PROTECTION REQUIRED”.
Each affected employee shall…
NOTE: Once individuals develop allergic symptoms, disposable surgical-style masks are usually NOT effective, and it is recommended that National Institute of Occupational Safety and Health (NIOSH) certified dust-mist respirators or filtered airhood devices be used. Effective use of these devices requires fit testing by the medical surveillance center and must receive training on how to use the device.
In addition to that noted above additional PPE may be required for the type of research being performed. An employee’s supervisor or OHS specialist can provide them with more information on the appropriate PPE required for the type of work or research being performed. Make sure that your selection of PPE fits properly and is appropriate for the job task.
Purdue University provides emergency safety showers in case a person needs to remove a chemical contaminant from their body. To use a safety shower, first remove all contaminated clothing, then stand under the shower and pull the handle. Employees should stay under the shower until emergency medical personnel arrive. REM test safety showers on an annual basis.
Purdue University provides eyewash stations in case an employee needs to flush contamination from their eyes. If a contaminant gets into an employee’s eyes, they should go to the eyewash station and place their eyes between the eyewash nozzles and push or pull the handle for water. The eyes should be flushed for at least 15 minutes or until emergency help arrives. The group responsible for the area in which the eyewash stations are located should test the stations weekly. After testing them, initial and note the date of the test on the tag attached to the eyewash. Report problems found with eyewash stations to REM at 46371.
Exposure to potentially hazardous biological, chemical, radiological, or physical agents should be monitored. Protective devices should be used when possible, and other safety practices consistent with current safety guidelines should be adopted. Potentially hazardous chemicals in the animal laboratory or care room may be found in disinfectants, cleaning agents, or pesticide, and as feed and bedding contaminants.
Hands should be washed after removing gloves, handling chemicals, infectious materials, animals, and before leaving the laboratory. A certified biological safety cabinet and gloves should be used when handling infectious materials and a certified fume hood used when handling toxic materials. All work surfaces—after use and daily—should be decontaminated. All contaminated materials should be decontaminated (by autoclaving or chemical disinfection) before washing, reuse, or disposal. The decontamination procedure will vary with the agent.
Laboratory animal facilities use the following methods to contain hazards:
The Biological Safety Cabinet (BSC) is a special and unique piece of laboratory equipment. Depending on the class, a BSC is used for working with low-, moderate-, or high-risk biological agents and materials. Cabinets are designed for personnel and environmental protection, and product protection. BSCs are among the most effective, as well as the most commonly used, primary containment devices in laboratories working with biohazardous agents. There are three classifications of BSCs, each designed for specific applications.
Training on the proper work practices and procedures for working with Biological Safety Cabinets is available by contacting REM at 4-7968 or 4-1496.
BSCs should be certified after installation, but before being used; whenever it is moved; and at least annually thereafter. Call REM at 4-7968 to schedule repairs and certifications of BSCs.
Sharps are items capable of puncturing, cutting, or abrading the skin such as razor blades, needles, syringes with needles, broken glass, test tubes, petri dishes and glass or plastic pipettes. Employees that routinely work with sharps and those who handle sharps waste should be aware of the risk of being punctured or lacerated during their workday. It is important for these employees to take precautions and properly handle waste materials in order to prevent injury and potential disease transmission. These employees should use appropriate PPE, tools, barrier protection, and engineering controls to protect themselves.
Sharps containers are disposed of by filling out the Biomaterials Pickup Request form attaching it to the container, and calling REM for pickup and removal at 4-0121
Employees who have potential for animal exposure are those that work with vertebrate animals, work in an animal housing area, maintain equipment, and building services staff assigned to animal housing buildings. Personnel who have both direct and indirect exposure to animals need to be provided with the appropriate awareness training. Understanding routes of disease transmission, disease or allergy signs and symptoms, personal protective equipment (PPE), waste handling, and emergency contacts is very important. The job tasks will determine the potential exposure, i.e., working on a ventilation system will have a greater exposure potential then emptying the trash. The OHS specialist and REM can provide a risk and hazard assessment before your exposure to the potential hazard.
The University’s Institutional Biosafety Committee (IBC) is the campus-based committee that has the responsibility for reviewing and approving all proposals, activities, and experiments involving recombinant DNA, biohazardous materials, and unfixed Human tissues, cell lines, or fluids. The IBC reviews processed protocol applications that deal with Class II or higher biohazards, unfixed human blood or tissues, or recombinant DNA materials.
The policy is directed to include all persons under age 18 whether students, employees, or volunteers. Minors 14 years of age and under are NEVER PERMITTED inside of any research laboratory, greenhouse, or animal facility at the Purdue University unless enrolled as a Purdue student. An exception is allowed for Purdue sponsored programs which are designed for youth under the age of 15 and which have documented training policies.
An animal bite or exposure is defined as having one's skin pierced or abraded by animal teeth or claws, or by coming in contact with animal saliva or tissue on abraded skin, eyes, or mucus membranes. Bites and scratches are potentially dangerous not only from the physical damage but also for the potential of contracting zoonotic disease or allergic reactions.
To protect from animal bites or scratches, employees should utilize sedation, anesthesia or a restraining device when possible. They should also use the proper animal handling procedures for the species. Also, be aware of the animals comfort zone and how far it can reach out to bite or scratch.
Since animals can bite through latex gloves, employees should use a thicker over-glove when appropriate. Employees should also consider using a two-person team for complex procedures.
Potential exposure to serious zoonotic diseases, such as rabies, is monitored by the Indiana State Department of Health, and by the Purdue Animal Exposure Occupational Health Program (AEOHP). Animal bites are a state reportable health event. The Indiana State Department of Health "Animal Bite Report - Report of Rabies Prophylaxis" form must be completed by the health care provider, you must also complete the First Report of Injury form located on REM’s website.
An allergen is a substance, usually a protein that can cause your immune system to react as if you are being infected with a cold virus. Allergies and the development of allergies are perhaps the most common hazard associated with working with and around animals. Allergies to animals are a common and important occupational health problem for persons who care for or work with animals. Allergic hazards are associated with breathing or contacting animal hair, dander or protein allergens (among others).
Species most commonly implicated in allergies are cats, dogs, rabbits, mice, rats, birds, hamsters and guinea pigs are the most frequently implicated allergy causing species. Exposure to animal related allergens (fur, saliva, hair, dander, and protein from urine) may occur by inhaling contaminates or by direct contact. Direct skin contact when handling animals may result in wheals, urticarial, and more chronic symptoms such as eczema. Bites and scratches must be avoided.
The animal allergens can originate from shed dander and hair, or can be contained in urine, saliva, serum, or tissues. Allergies develop after you have been exposed to foreign proteins. Example: after being exposed to an animal protein found in rat urine you can become sensitized (your body is ready to fight this reoccurring foreign protein) Scenario: you enter a room housing rats just like you have done many, many times before but this time your immune system recognizes the rat urine protein as an invader and is prepared for battle. Special cells called "mast cells" release chemical defense agents that have been stored up just for this occasion (notably histamine). This release of defense chemicals is what causes the hives, sneezing, nasal drainage, and shortness of breath. All people are capable of having an allergic reaction, some more so than others.
Allergy symptoms include rhinitis (runny nose and sneezing similar to hay fever), conjunctivitis (irritation and tearing of the eyes), asthma, and/or dermatitis (skin reactions). Some people react quickly and begin having temporary symptoms within 10 -15 minutes after exposure. Others can have a delayed reaction starting several hours after exposure. There are some people, due to their genetic composition, who react by developing more severe allergy related diseases like asthma or dermatitis. Allergic reactions are implicated if symptoms are reduced or stop after leaving the work place.
Avoiding allergens completely may be the best way to control animal allergies. For these individuals a variety of practices can help reduce contact with the offending allergens. These include the use of masks and other protective clothing, housing animals in filter-top-cages, and the use of other filtered and ventilated caging systems, improving ventilation, avoiding recirculation of animal room air, increasing the frequency of cage cleaning, and reporting problems promptly to a supervisor or physician.
Humans are not usually susceptible to infectious diseases suffered by animals. However, there are some important exceptions. Organisms carried by normal-appearing animals may, on some occasions, produce significant disease in people. Such infections, shared by animals and man, are called zoonoses. While the animals have natural resistance to these microorganisms, humans with no previous exposure to the agent lack this protective immunity. Therefore, always be aware of possible consequences when working with each type of animal and take precautions to minimize the risk of infection. In the event that you become ill with a fever or some other sign of infection, let your physician know of the work you do with animals. This history of exposure is often critical in the recognition of disease conditions.
Some specific zoonotic diseases and the animals associated with them are described in more detail in Appendix A. Common sense practices can lessen the risk of infection in general. These include cleanliness in routine tasks around animals and hand washing after completion of animal work. Workers should also: wear gloves to protect against accidental self-inoculation; substitute manually operated pipettes for needles and syringes and cannula for needles; take enough time to give injections properly; and use a two-person team to inject animals, if necessary. Do not re-cap needles; discard them in a container designed for proper disposal of contaminated “sharps.” For procedures such as necropsy, bedding changes, and tissue and fluid sampling, use biological safety cabinets, physical containment devices, or other personal safety gear when appropriate.
The scope of possible zoonotic infections is quite large and not all examples are described here. Zoonotic diseases are those that can be transmitted from animals to humans. Although zoonotic diseases are not common, the prevention, detection, and eradication of zoonotic diseases from the animal facility are a primary concern of the entire animal care staff. It is important to remember that, unfixed animal tissues, animal waste materials, as well as the animals themselves may also transmit zoonotic disease. Pregnant workers can be at very high risk for certain animal diseases. Awareness, common sense, PPE, and avoidance will protect against and prevent most of these diseases.
Below are examples of diseases associated with common vertebrate animals. Use of proper PPE can reduce these risks. All personnel should be aware that laboratory animals (particularly rats, rabbits, guinea pigs, hamsters, and cats) are sources of potent allergens to sensitized persons.
Ruminants such as sheep, goats, and cattle can present a significant risk to animal handlers for exposure to a highly transmittable disease causing organism known as Coxiella burnetii (“Q fever”). “Q fever”, a potentially serious human disease caused by the rickettsia Coxiella burnetii, and historically was common in those drinking unpasteurized milk or working in slaughterhouses handling animal carcasses (cattle, sheep, and goats). It is an airborne rickettsial (organism smaller than bacteria) that causes chills and fever in adults and horrible birth defects for the fetus. It is now known that the organism is shed abundantly from the placental membranes of sheep. In pregnant ruminants, Coxiella burnetii builds up to enormous numbers in certain tissues and fluids, i.e., the uterus or womb, the placenta, the mammary glands or udders, birth fluids, and milk. Animal handlers, farm workers, students, and visitors must be made aware of the potential disease development that could result from exposure to the placenta, amniotic fluids, milk, and feces from birthing ruminants.
Individuals can be infected with Q Fever unknowingly, or mistake their symptoms for the flu. Often, it is impossible to diagnose without laboratory tests. Q Fever strikes as a sudden illness, affecting a large number of people in the same workplace. Common signs and symptoms include:
More serious complications can affect the immune-compromised, individuals with heart abnormalities, and pregnant human females, i.e., endocarditis and miscarriage or premature birth. Person to person transmission occurs rarely, but contaminated clothing can transmit the organism. Individuals should notify their physician or their occupational health provider if signs and symptoms of Q Fever arise.
Personal Protective Equipment such as OB Sleeved gloves, nitrile gloves, N-95 dust mask, and protective outerwear should be available for individuals exposed to ruminants, especially during birthing procedures. Hands should always be washed with a disinfectant soap immediately after contact with the animals, tissues, or wastes.
Surfaces contaminated by ruminant birthing fluids and tissues can be decontaminated with a 1 to 10 bleach and water solution. Contaminated bedding can be composted and tissues can be incinerated or buried. Take care not to produce dust when cleaning and/or decontaminating. If dust production in unavoidable, the use of a properly fitting N-95 dust mask is suggested.
Be aware of the sources of Q Fever; especially fluids and tissues from pregnant ruminants. Only allow authorized individuals who have been made aware of the potential biohazards to enter the ruminant housing and birthing area.
Report any suspect allergic reactions or zoonotic illness to your Supervisor and to the OHS Specialist. If you have signs and symptoms of an animal exposure related allergy alert your supervisor and the OHS specialist and he/she will send you a risk assessment form to complete if you have not already completed one.
Employees have the right to know the hazards of the chemicals and products they work with. The Hazard Communication Standard (29 CFR 1910.1200) was initially promulgated to protect employees handling chemicals during chemical manufacturing or as a chemical user in the manufacturing sector (SIC Codes 20-39). The standard was expanded to cover the non-manufacturing sector including universities.
The state of Indiana, under the authority of Indiana Occupational Safety and Health Administration (IOSHA), 402 West Washington Street, Room W-195, Indianapolis, IN 46204, has adopted the Federal Hazard Communication Standard.
What is the purpose of a written Hazard Communication Program (HCP)?The standard requires employers to develop and implement a written hazard communication program for their workplaces. The written hazard communication document provides information to employees about their rights under the law and details how the program is administered at their workplace. It specifies the methods for providing employee training so they 1) recognize and understand the hazards of the chemicals they work with, and 2) recognize and understand the labeling system for chemicals and products they use.
For more information please contact REM.
Radiological and Environmental Management (REM)
IOSHA Right-to-Know Law (29 CFR 1910.1200)
550 Stadium Mall Drive
West Lafayette, IN 47907-2051
Radiological and Environmental Management (REM) act as an agent of the OSHA Compliance Officer to certify a safety program. Further information on Physical Hazards can be found on the REM website under Integrated Safety Plan (ISP).Most of the health problems occurring in an animal facility involve accidents unrelated directly to animals. These problems are very common, and are prevented by knowledge of dangers, proper cautions, and appropriate signage.
Radioisotopes are radioactive forms of normally nonradioactive elements. They emit low levels of radiation, which makes them valuable as tracers in biological investigations of metabolic processes. Usually these types of isotopes are dangerous only if contacted directly. The use of some radioisotopes, however, requires stringent precautions and safety measures.
Pathogens are live infectious bacteria, viruses, fungi, or parasites that pose a threat to humans and animals. Some pathogens and their diseases are, for example tuberculosis in monkeys, cryptosporidiosis in cattle or Q-fever in sheep. Many pathogens are blood-borne. Other pathogens are utilized as a component of some research studies. The goal of OSHA’s Bloodborne Pathogens standard is to minimize or eliminated occupational exposure to blood or potentially infectious materials. The standard covers employees with reasonably anticipated skin, eye, mucous membrane, or parenteral contact with blood or other potentially infectious materials. To comply with the standard the University has developed and exposure control plan and offers covered employees the Hepatitis B vaccination. All University staff that has potential exposure to human blood, body fluids, unfixed human tissues, or animal with human tissue or tumor implants or grafts must take Bloodborne Pathogens training on an annual basis. For more information on the University Bloodborne Pathogens Programs, please contact IBC. In all cases, standard safeguards and procedures should be developed by the facility management to protect technical staff and investigators.
Mutagens are substances that cause changes in chromosomes and thereby induce the occurrence of mutations. Examples of such substances are high doses of X-rays and some chemicals. Teratogens affect the embryo or fetus. Carcinogens are substances that can produce cancer directly.
Compressed gas is any material or mixture having, when in its container, an absolute pressure exceeding 40 psia (an absolute pressure of 276 KPa) at 70°F (21.1°C) or, regardless of the pressure at 70° F (21.1° C) having an absolute pressure exceeding 104 psia (an absolute pressure of 717 kPA) at 130° F (54.4° C).
All compressed gas cylinders, either in use or in storage (empty or full), shall be tightly secured by a strap, chain, non-tip base or other approved means. When securing the compressed gas cylinders, six (6) cylinders are the highest number that can be secured on a single chain/strap securement. When in use, all cylinders must be equipped with an appropriate regulating device. Special storage requirement is based on the total volume of compressed gas in an area. Partially full compressed gas cylinders containing residual gases shall be considered as full and are subject to the same controls and storage conditions.
Toxins are poisonous substances produced by bacterial, plant, or animal cells. Some bacteria for example, produce toxins (e.g. tetanus), and castor bean plants produce a toxin called ricin. The Occupational Safety and Health Administration (OSHA) requires that laboratory employees be made aware of the Chemical Hygiene Plan (CHP) at their place of employment (29 CFR 1910.1450).
The Purdue University Chemical Hygiene Plan and Hazardous Materials Safety Manual serves as the written Chemical Hygiene Plan for laboratories using chemicals at Purdue University. The CHP is a regular, continuing effort, not a standby or short term activity. Departments, divisions, sections, or other work units engaged in laboratory work whose hazards are not sufficiently covered in this written manual must customize it by adding their own sections as appropriate (e.g. standard operating procedures, emergency procedures, identifying activities requiring prior approval.
It is the policy of Purdue University to take every reasonable precaution to provide a work environment that is free from recognized hazards for its employees in accordance with the General Duty clause of the OSHA Act (Public Law 91-596, Section 5(a)(1). Purdue University is also required by the OSHA laboratory standard to ensure that the necessary work practices, procedures and policies are implemented to protect employees from all potentially hazardous chemicals in use in their work area.
Purdue University has established the Chemical Management Committee with the responsibility to promote safe and proper chemical management at all Purdue University Campuses and related facilities.
For more information on CHP please see REM’s website under Booklets and Guidelines
Pregnant caretakers without immunity to toxoplasmosis should not be exposed to experimentally infected animals and should avoid contact with cats and places that cats are known to defecate because of the risk of congenital Toxoplasma infection. Avoid cat feces. Wear gloves when working in areas potentially contaminated with cat feces or fresh necropsy specimens, which also can contain infectious Toxoplasma organisms. Wash hands thoroughly after handling any potential source of infection.
Coxiella burnetti, a rickettsial organism and the cause of Q fever in humans, can infect sheep, cattle, goats, and cats. This rickettsia has a predilection for the uterus and mammary glands of these animals and can be found in birthing products and raw milk. Q fever can cause pneumonia, fetal death, hepatitis and chronic endocarditis. Pregnant women should minimize exposure to uterine and placental discharges, especially those of sheep. Dusty situations can aerosolize this resistant organism making exposure without animal contact possible in areas of high sheep density.
Another zoonotic organism of importance to pregnant women is the bacterium Listeria monocytogenes. Pregnant women are more likely to contract listeriosis than other healthy adults. The organism is found in soil, water and manure. Infected animals can carry the organism without appearing ill. The bacterium can be found in a variety of raw foods such as uncooked meats and vegetables or in processed foods that are contaminated after processing such as deli cheeses and meats. Complete washing of fresh vegetables and fruits and thorough cooking of all food along with washing hands frequently will help reduce the likelihood of infection.
In rare cases, pregnant women have contracted Chlamydia psittaci from psittacine birds or from exposure to birth fluids or membranes of sheep or goats. This exposure can result in gestational psittacosis and subsequent pneumonia, sepsis, and placental insufficiency. Pregnant women should not be exposed to sheep, goats or psittacine birds of unknown or positive Chlamydia psittaci status.
Avoid working with hazardous agents or exposure to radiation or chemicals that are known to be teratogenic. Persons who are or are planning to become pregnant, are immune-compromised for any reason (AIDS, chemotherapy, steroid use, chronic disease), have neuromuscular or musculoskeletal problems, have diabetes, are missing a spleen, or have other illness that may place them at extra risk should contact their physician before being involved with the use or care of animals.
Dogs and cats used at Purdue University are vaccinated against rabies. Nevertheless, some risk of rabies exposure exists because the observation period may be too short to allow typical signs of the disease to develop in the animal. Individuals working with random source dogs and cats should be vaccinated against rabies and have regular titer checks.
Parasite infections such as visceral larval migrans, some tapeworm infections, and sarcoptic mange, are a potential risk to those handling dogs and cats, as are bite wound infections and ringworm (a fungal disease of the skin).
Cat scratch disease is a zoonotic infection characterized by inflammation of regional lymph nodes that follows the formation of a skin papule in the site of a cat scratch or bite. While the prognosis usually is excellent and the disease in most cases is self-limiting, an examination by an occupation health physician is recommended.
Q fever, a potentially serious human disease caused by the rickettsia Coxiella burnetii, was formerly quite common in those drinking unpasteurized milk and in slaughterhouse workers exposed to the tissues of freshly slaughtered cattle, sheep, and goats. It is now known that the organism is shed abundantly from the placental membranes of sheep. This route of exposure has caused Q fever pneumonia in laboratory workers. Personnel working with sheep used in reproductive research or other studies should take extra precautions. A history of exposure to sheep, goats, or cattle is important in establishing the diagnosis. Infected persons can be treated effectively with antibiotics. Erysipelas in pigs can be transmitted as a severe focal skin infection to man, and pigs showing characteristic lesions should be handled with care. Similarly appearing, though less severe, skin lesions also are seen, especially on the hands, after contact with sheep and goats infected with contagious ecthyma (“orf”) and vesicular stomatitis.
Rabies also can be a threat in large animals such as cattle, horses, and pigs.
Unusual research species pose other risks. Birds can carry diseases such a psittacosis, avian tuberculosis, cryptococcosis, or Newcastle disease. Only inspected and properly quarantined birds should be used in research studies or teaching demonstrations. Reptiles frequently are asymptomatic carriers of Salmonella.
(e.g., mice, rats, guinea pigs, hamsters, gerbils)
Contact with rodents requires precautions against such diseases as tapeworm infection, lymphocytic choriomeningitis, rat-bite fever, and ringworm. Additional concerns for individuals exposed to wild rodents are bubonic plague (particularly in certain geographic areas), toxoplasmosis, leptospirosis and hantavirus.
Lymphocytic choriomeningitis, a rodent neurologic virus, is transmissible to man; be careful when handling rodents as well as potentially infected materials, such as bedding, feces, or rodent derived tissues or cell cultures.
Laboratory bred and raised rodents are not normally expected to carry rabies.
There should be methods for monitoring exposure to potentially hazardous agents in the laboratory. Use personal protective equipment when required and adopt other safety practices consistent with current guidelines. Potentially hazardous chemicals in the animal laboratory include disinfectants, cleaning agents, or pesticides as well as substances utilized in experiments.
Wash hands after handling chemicals, infectious materials, or animals. Use personal protective equipment when required and a biological safety cabinet when handling infectious materials and a fume hood when handling toxic materials. Decontaminate all work surfaces daily, as well as all infectious materials or equipment before disposal or reuse, either by autoclaving or chemical disinfectant.
For further information on working with hazardous agents, contact Radiological and Environmental Management (REM) at 46371.
The Campus Emergency Preparedness and Planning Office is the focal point to oversee emergency preparedness and planning activities on Purdue University Campus. The office is tasked with the oversight of the University’s “All Hazards” Integrated Emergency Operations Plan that will be used in the event a natural disaster or a human-made incident strikes the campus.
A key part to Purdue’s campus preparedness is the Purdue alert, or the Emergency Warning Notification System. We have instituted a multi-layered communication approach to spread the word on emergency events.
The Emergency Preparedness Office also provides technical assistance and direction for Building Emergency Plan (BEP) development. The Building Deputy or an individual designated by the department head will develop the BEP and submit it to the Campus Emergency Preparedness and Planning Office for review, distribution to the Fire Department, and posting to the Emergency Operations Center building binder.
Additionally, the Emergency Preparedness Office annually revises and coordinates the Purdue Emergency Procedures Handbook. All Purdue faculty and staff should be familiar with the handbook and use the detailed procedures when responding to emergencies.
Lastly, faculty and staff should periodically review Purdue’s emergency preparedness Web site (www.purdue.edu/emergency_preparedness) for any new preparedness and planning information and as a source for current emergency preparedness material.
If an employee discovers a fire or emergency inside a building, they should activate the manual alarm pull station. Pull stations are located near emergency exits in the building. Once an employee is a safe distance away from the emergency, they should call University police at 911 or non-emergency 4-0470
If any person is injured to the extent that he/she cannot be sent to the Student Health Center or the emergency room, the supervisor should:
For any further information, contact the Occupational Health and Safety Specialist, Carol Oteham, 61334, email@example.com.
American National Standard Institute (ANSI) standards
National Institute for Occupational Safety and Health (NIOSH)
The following pages are a list of Zoonotic Disease and their Transmission
|Animals||Common Name||Organism||Risk/ Concern||Common mode/ method of Transmission||Human Symptoms of Infection||Active/ Passive Transmission|
|Mammals||Rabies||Rabies Virus||Low/Moderate and up to High in wildlife||Primarily bite from infected animal; any salivary contamination to open skin on a human||Incubation in human varies, 10 days to months. May produce: Nausea, vomiting, headache or mild fever. Paresthesia and pain at sit of bite wound or inoculation site. Neurological changes cause furious/ aggressive behavior or general paralysis nearly always fatal||Active|
|Sheep, Cattle, Goats||Q-Fever||Coxiella burnetti||Moderate||Organism shed in urine, feces, milk and especially birth products of domestic ungulates. Organism is resistant to drying and can persist for months. Aerosol is a major means of transmission.||Sudden fever, retrobulbar or frontal headache, chills, sweating, myalgia, weakness, pneumonitis, endocarditis, hepatitis||Passive|
|Rodents, birds, rabbits, guinea pigs, mice, cats, NHPs, sheep, swine, goats||Yersinia (gram negative and gram positive)||Pseudotuberculosis||Moderate||Direct contact, or fecal contaminated food or water||Acute watery diarrhea, mesenteric lymphadenitis which can be confused with appendicitis, fever, headache, pharyngitis, anorexia, vomiting erythema nodosum (in about 10% of adults), post-infectious arthritis, iritis, cutaneous ulceration, hepatosplenic abscesses, osteomyelitis, and septicemia.||Passive|
|Rabbits, dogs, cats||Ringworm||Microsporum and Trichophyton||High/Low||Direct contact||Generally, scaling, hair loss or breakage; occasional itching; less frequently, erythema, induration, crusting, suppuration||Passive|
|Cats, Cat feces||Toxoplasmosis||Toxoplasma gondii||Moderate||Direct contact with cat feces as well as exposure to garden soil of sandboxes where infected cats may have defecated.||Usually, lymphadenopathy, fever, headache, myalgia, stiff neck, anorexia; occasional arthralgia, maculopapular rash, mental confusion, if pregnant: still born, abortion of fetus.||Passive|
|Dogs, Sheep, Cattle, Goats, Swine||Brucellosis||Brucella||Low/moderate-high||Ingestion of unpasteurized milk, contact with infected animals especially aborted fetuses, fluids or membranes or urine, possibly airborne||Gradual onset, undulating fever, chills, sweats, headache, myalgia, fatigue, backache, weakness, weight loss, can be chronic with recurrent fevers. And associated symptoms||Passive|
|Dogs, wild rodents, rabbits||Rocky Mountain Spotted Fever||Rickettsia rickettsii||Low/moderate||Infection through broken skin of crushed mites and ticks and their feces||Sudden onset fever with chills, headaches, severe muscle pains, photophobia and meningism for four weeks. A red, morbilliform rash develops within 3-5 days of onset of fever and with hemorrhages spreading on limbs.||Passive|
|Dogs, rabbits, ruminants, cats, birds||Pasteurellosis||Pasteurella||Low/moderate||Wound infection from bites or scratches.||Manifest in one or more of the following Syndromes: wound infections, upper/lower respiratory tract infection, abdominal/pelvic infections, fatal sepsis||Active|
|Mostly Herbivores||Tetanus (Lockjaw)||Clostridium tetani||Low/very high||Puncture wound, bite or scratch transmission via contact with contaminated soil, GI flora of Herbivores||Intermittent to continuous tonic muscular spasms; terminal asphyxia due to inability to move the diaphragm muscle.||Active|
|Dogs, cats||Septicemia||Capnocytopha-gia, Canimorsus and C. Cynodegmi||High in Immunocompromised or splenectomized individuals||Bite, even minor bite||1-8 days (5 on average) from time of bite to onset of symptoms which may include: Fever, chills, myalgia, vomiting, diarrhea, abdominal pain, mental confusion, seizures, gangrene. Greater than 30% fatality rate.||Active|
|Rodents||Lymphocytic choriomeningitis||LCM virus||Low/high||Direct exposure to fresh urine, droppings, saliva or nesting materials or bite of infected rodent.||Fever, myalgia, malaise, occasional stiff neck, headache, sleepiness, unusual skin sensations (paresthesia), paralysis; usually self-limiting. Some fatalities.||Passive|
|Most Species (animals and birds)||Salmonellosis||Salmonella||Low/moderate||Contaminated food and water and by direct contact. Common contaminant of sewage. Animal feed containing animal by products (raw meal that has not undergone the pelleting process)||Diarrhea, vomiting, low-grade fever; may progress to dehydration, prostration, death; very high fever, to septicemia, splenomegaly, headache in humans||Passive|
|Rodents, guinea pigs, Rabbits, dogs, cats, cattle, sheep, swine, monkeys||Pneumocystis Pneumonia||Pneumocystis Carinii||High for Immunocompromised individuals||Host specific parasite||Generally seen only in those with serious underlying disease, or suppressed immune system; pneumonia, dyspnea, nonproductive cough, moderate fever, tachypnea||Passive|
|Ground squirrels, wild caught rodents||Plague (Bubonic and Pneumonic)||Yersinia pestis||Low/high||Bite of an infected flea or working with animals infected with plague and breathing in tiny droplets of water contaminated with Y.pestis.||Bubonic-fever, chills, nausea, diarrhea or constipation, headache, meningitis, tachycardia, coma, regional, lymphadenopathy. 60% fatality rate if untreated. Pneumonic-cough and dyspnea with mucoid to bright red sputum; may progress to septicemic form, with vascular collapse, hemorrhagic rash. 95% fatality rate in these two forms if untreated.||Passive|
|Rats, mice, field moles, guinea pigs, gerbils, squirrels, rabbits, hamsters, reptiles, NHPs, livestock, and dogs||Leptospirosis||Weil's diseases, Hemorrhagic jaundice||Low/moderate||Direct contact with abrasions or with urine or aerosol exposure during cage cleaning are most common.||Biphasic illness, weakness, headache, myalgia, malaise, chills and fever. Leukocytosis, painful orchitis, conjunctival effusion, and rash.||Passive|
|Pigs||Streptococcosis||Streptococcus suis and S. zooepidemicus||Low||Handling infected mean. Direct contact with domestic animals and drinking raw milk.||Fever and occasionally meningitis. May cause upper respiratory tract symptoms, cervical adenitis, pneumonia, endocarditis and nephritis.||Passive|
|Vertebrates||Coli bacillosis||Escherichia coli||Low/moderate||Direct contact with infected vertebrates or to contaminated, poorly heat treated meat or milk originating from infected animals.||Pneumonia, urinary tract disease, watery diarrhea, abdominal pain, +/- short period of fever||Passive|
|Wild or unscreened rodents||Hantaan virus (Korean Hemorrhagic Fever)||Hantaan virus||Low/high||Direct contact with urine, droppings, or stirred up in dust and breathed in from places where infected animals have nested.||Incubation from 5-35 days post exposure. Subtle onset; malaise, fever with neurological disturbances, common renal shutdown, headache, tremors of tongue and extremities, shock. 30-40% fatality rate.||Passive|
|Cats||Cat Scratch Fever||Bartonella spp||Low/Moderate||Cat scratch or bite.||Erythematous papule at inoculation site followed by 1 regional lymphadenopathy; malaise, anorexia, myalgia, nausea.||Active|
|Rats||Rat Bite Fever (Haverhill)||Strep. Moniliformis||Moderate/moderate||Rat bite.||May cause high fever, chills, vomiting, sore throat myalgia, headache, backache and/or possible disturbances of consciousness.||Active|
|Rats||Rat Bite Fever (Sodoku)||Spirillum minus||Moderate||Rat bite, contamination during oral surgery||Bite wound may heal initially then develop pain, edema to firm swelling, turn purple or ulcerate up to several weeks post original bite. Other symptoms: headache, diarrhea, something, myalgia, myocarditis, hepatitis, meningitis are possible.||Active|
|Fish, birds, swine, horses, ruminants, guinea pigs, ferrets, gerbils, rabbits, and chinchillas||Listeriosis||Circling disease||Low||Ingestion of unpasteurized milk and cheese and contaminated vegetables; direct contact with infectious material or soil contaminated with infected animal feces||Fever, headache, nausea, vomiting, endocarditis, granulomatous lesions in multiple organs, cutaneous involvement, coryza, conjunctivitis, metritis with abortion, sepsis and meningitis. Granulomatous lesions and abscesses occur in the liver and other organs and beneath the skin.||Passive|
|Armadillo||Leprosy||Mycobacterium leprae||Low||Not well known, but direct contact with infected skin through wounds or abrasions and by aerosols||Range from single, localized lesions to diffuse, generalized infiltration of skin.||Passive|
|NHPs, cattle, birds||Tuberculosis||Mycobacterium spp||Moderate/High||Infected animals or infected tissue primarily via the aerosol route. Also via ingestion or cutaneous inoculation of the bacilli.||Pulmonary-productive cough, fever, weight loss, fatigue, night sweats, chest pain, hemoptysis Extrapulmonary-cervical lymphadenitis, meningitis, osteomyelitis, pericarditis, infections of most other organs.||Passive|
|Non-human primates (NHPs)||Simian Hemorrhagic Fevers (Ebola, marburg)||Rhabdovirus||Low/very high||No carrier state has been determined.||Fever, malaise, headache, sore throat myalgia, vomiting, diarrhea, conjunctivitis, hemorrhages. High % fatalities even with therapy.||Passive|
|NHPs||Shigellosis||Bacillary dysentery||Low||feces and contaminated objects and direct contact||Incubation less than 4 days. Fever and abdominal pains, followed by diarrhea and dehydration for 1 to 3 days. Main symptom is tenesmus; in serious cases, stool containing blood, mucus and pus.||Passive|
|NHPs||Yaba Virus (Uaba Monkey tumor virus)||Pox virus||Moderate||Direct contact with infected animal blood or through a bite||Papulae develop to subcutaneous tumors on limbs, pox lesions hands, feet, face, ears; regional lymphadenopathy.||Passive|
|NHPs||Herpes B, B-virus||Cercopithecine herpesvirus 1||Moderate/High||Contact with infected NHP saliva, tissues, needle sticks||Vesicle/blister at site of entry, regional lymphadenopathy, possible paresthesias, pruritus, fever, headache, flu like symptoms, mengioencephalitis. Nearly 100% FATAL, without early treatment.||Active|