Resources - In-Vitro Studies

Mechanism of Action

The Bindley Bioscience Flow Cytometry and Cell Separation Facility provides advanced cell and particle analysis and sorting using flow cytometry based technology. The facility provides training in flow cytometry analysis and sorting techniques as well as expert consultation for assay and protocol development for flow cytometry analysis and cell separation. Instruments include the Beckman Coulter Quanta SC, the Beckman Coulter FC500, the iCyt Reflection Sorter and the Cyntellect LEAP. Contact: James Leary, jfleary@purdue.edu, or Jill Hutchcroft, hutchcroft@purdue.edu, or call 765-494-3505.

Drug Affinity and Selectivity

The Integrated Screening Technologies laboratory, part of the Biomolecular Screening and Drug Discovery facility, provides researchers with capabilities to create new approaches to discover biological, synthetic, and natural compounds that can lead to new drugs. Scientific approaches include drug design and synthesis with novel chemistries; nanomaterials for tagging and imaging drugs and their effects; and metabolomic/proteomic profiling for evaluating response of cells to drugs. Contact: Larisa Avramova, 765-496-3102 or lva@purdue.edu.

The Biophysical Analysis Laboratory, located in the Bindley Biosciences Center, provides complete biophysical analysis of macromolecules. Two analytical ultracentrifuges allow researchers to determine kinetic parameters, stoichiometry of binding, size distribution, shape, oligomerization state and other biophysical measurements. Complementing these traditional biophysical methods, the BAL also provides mass spectrometric analyses at both the protein and peptide levels. Contact: Lake Paul, lpaul@purdue.edu or 765-494-4960.

Cellular Imaging

The Life Sciences Electron Microscopy Facility is a full service electron microscopy core with both service and individual use options. Equipment includes two field-emission SEMs equipped with cryo, EDX, low vacuum, and tensile stage. Also available are 2 TEMs, routine and cryo sample preparation instrumentation, and other support equipment. Basic light microscopes are equipped with color digital cameras and optics for Bright-field, Nomarski DIC contrast, Fluorescence, and stereo imaging. Contact: Christopher Gilpin, gilpin@purdue.edu.

A joint effort of the Colleges of Agriculture, Health and Human Sciences, Engineering, Science, Veterinary Medicine, the Life Sciences Fluorescence Imaging Facility provides trained users access to the Zeiss LSM 710 Confocal Laser Scanning Microscope, the Zeiss Axio Observer Epifluorescence Microscope and the Intavis InSitu ProVS preparative robot. Contact: Christopher Gilpin, gilpin@purdue.edu.

The Bioscience Imaging Facility provides the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals. Facility users receive individualized training as well as project specific guidance for optimal data acquisition. Instrumentation includes: Nikon A1R Confocal, Nikon A1R MP Multiphoton, Nikon Clt Confocal, Nikon Wide-field, Caliper IVIS Lumina II, MILabs V-SPECT-II/CT. Contact: Aaron Taylor, abtaylor@purdue.edu.