Hollow Fiber Microfiltration of Microorganisms from Food and Water
Dr. Michael R. Ladisch and Dr. Eduardo Ximenes
Summary: The webinar examines the factors involved in designing and operating an automated hollow fiber microfiltration system for concentrating microorganisms from complex fluids that can rapidly foul membrane. Also discussed is how to combine pretreatment of fluids prior to microfiltration with an automatically controlled, cross-flow microfiltration system. This allows flow to occur on both retentate and permeate sides of 0.3mm diameter hollow fiber membranes, resulting in cells being concentrated by a factor of 500 to 1000 in two hours or less. This webinar also identifies principles and approaches to controlling fouling and explains the attendant microfluidic phenomena. Prototype systems currently being validated will be discussed.
C3D: Moving Laboratory Research on Pathogen Detection into Commercial Practice
Dr. Michael R. Ladisch and Dr. Eduardo Ximenes (Purdue University)
Summary: An essential component of pathogen and other microorganism detection in food and water is sample preparation so that the pathogen is at a sufficient concentration and can be readily detected. The approach discussed here uses the C3D instrument. It involves rapid microfiltration of liquid samples derived from either food or water, where the system removes water through microfiltration thereby concentrating the microorganisms. This results in a 1000-fold concentration of microorganisms. When followed by micro-centrifugation, a pellet of viable microorganisms results that may be probed for the presence of pathogens used in either antibody or PCR-based methods. This webinar presents progress toward the goal of providing an instrument for rapid detection of pathogens in the industry, and discusses the steps involved in translating the technology to industry laboratories.