- B.S., Agricultural Biochemistry at Iowa State University in 1992
- Ph.D., Nutritional Biochemistry at Purdue University in 1999
- Post-Doc, Lipid Metabolism at Gladstone Institute of Cardiovascular Disease in 2002
- Post-Doc, Cardiology and Gastroenterology at Washington University School of Medicine in 2005
Awards & Honors
- E.L.R. Stokstad Award from American Society of Nutrition, 2013
Activities & Memberships
- Member, American Diabetes Association, 2012 - Present
- Member, American Society for Biochemistry and Molecular Biology, 2009 - Present
- Member, American Society for Nutrition, 2001 - Present
- Member, American Heart Association, 2001 - Present
The long-term goal of the Buhman laboratory is to identify novel factors that regulate dietary fat sensing, metabolism or absorption that may be exploited for preventive and therapeutic interventions for obesity, diabetes, and heart disease. Research in the Buhman laboratory focuses on trafficking and metabolism of digestive products of dietary fat within the absorptive cells of the small intestine, enterocytes. Projects in the Buhman laboratory are currently addressing how diet, drugs and genetics affect chylomicron synthesis and secretion, cytoplasmic lipid droplets synthesis and metabolism, and fatty acid oxidation by enterocytes. Recent publications from the Buhman laboratory highlight important functions of diet, drugs, and genetics in regulation of dietary fat processing within enterocytes that results in effects related to metabolic diseases such as body weight, blood lipid concentrations, and hepatic steatosis.
- D’Aquila, T.D., Sirohi, D., Grabowski, J.M., Hedrick, V.E., Paul, L.N., Greenberg, A.S., Kuhn, R.J., Buhman, K.K. (2015) Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge. Plos One. 10 (5):e0126823. PMID 25992653.
- Hung, Y., Linden, M.A., Gordon, A., Rector, R.S., Buhman, K.K., (2015) Endurance Exercise Training Programs Intestinal Lipid Metabolism in a Rad odel of Obesity and Type 2 Diabetes, Physiological Reports. 3(1). PMID 2560212.
- Lee, H.J., Zhang, W., Zhang, D., Yang, Y., Liu, B., Barker, E.L., Buhmann, K.K., Slipchenko, L.V., Dai, M., Cheng, J.X. (2015) Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol. Sci. Rep. 5:7830. PMID 25608867.
- Uchida, A., Eustaquio, T., Leary, J.F., Cheng, J.X. and Buhman, K.K. (2013) Intestinal acyl-CoA:diacylglycerol acyltransferase 2 overexpression enhances postprandial triglyceridemic response and exacerbates high fat diet-induced hepatic triglyceride storage. Biochim Biophys Acta-Molecular and Cell Biology of Lipids. 1831(8):1377-1385. PMID: 23643496.
- Wilfling, F., Wang, H., Haas, J.T., Krahmer, N., Gould, T., Uchida, A., Cheng, J-X., Graham, M., Christiano, R. Frohlich, F., Liu, X., Buhman, K.K., Coleman, R.A., Bewersdorg, J., Farese, R.V. Jr., and Walther, T.C. (2013) Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Developmental Cell. 24(4):384-99. PMID: 23415954.
- Uchida, A., Whitsitt, M.C., Eustaquio, T., Slipchenko, M.N., Leary, J.F., Cheng, J., and Buhman, K.K. (2012) Reduced triglyceride secretion in response to an acute dietary fat challenge in obese compared to lean mice. Frontiers in Fatty Acid and Lipid Physiology. 3(26):1-10. PMID: 22375122.
- Uchida, A., Slipchenko, M.N., Cheng, J.X., and Buhman, K.K. (2011) Fenofibrate (FEN), a peroxisome proliferator activated receptor alpha (PPARa) agonist, decreases dietary fat alters triglyceride (TG) metabolism in enterocytes of mice. Biochim Biophys Acta - Molecular and Cell Biology of Lipids. 1811(3):170-6. PMID: 21215818.
- Lee, B., Zhu, J., Fast, A., Cheng, J-X., and Buhman, K.K. (2010) Intestine specific expression of acylCoA: diacylglycerol acyltransferease 1 (DGAT1) reverses the resistance to diet-induced hepatic steatosis and obesity in Dgat 1-/- mice. Journal of Lipid Research. 51(7):1770-80. PMID: 20147738.
- Lee, B-G., Zhu, J., Wolins, N., Cheng, J-X., and Buhman, K.K. (2009) Differential association of Adipophilin and TIP47 with cytoplasmic lipid droplets in mouse enterocytes during dietary fat absorption. Biochim Biophys Acta - Molecular and Cell Biology of Lipids. 1791(12):1173-80. PMID: 19698802.
- Zhu, J., Lee, B., Buhman, K.K., and Cheng, J.X. (2009) Direct Observation of a Dynamic Triglyceride Pool in Mouse Enterocytes during Dietary Fat Absorption by Coherent Anti-Stokes Raman Scattering Imaging. J. of Lipid Research. 50(6):1080-9. PMID: 19218555.
Books, Chapters & Monograph Publications
- Uchida, A., Lee, H.J., Cheng, J.X., and Buhman, K.K. (2013) Imaging cytoplasmic lipid droplets in enterocytes and assessing dietary fat absorption. In: Methods in Cell Biology. H. Yang and P. Li Ed(s). Ch.9. Pp.151-166. Elsevier, Amsterdam, The Netherlands.
- Buhman, K.K. (2012) Visualization of dietary fat absorption by CARS. In: Coherent Raman Scattering Microscopy. CRC Press. ISBN 9781439867655.
- Antalis, C. and Buhman, K.K. (2012) Lipoproteins and Cancer. In: Lipoproteins-Role in Health and Disease. TECH. ISBN 9789535107736.
|Principles of Food Preparation and Nutrition. Dr. Buhman teaches nutrition within this course.|
|NUTR 43700||Macronutrient Metabolism in Health and Disease: Metabolism of the macronutrients, carbohydrate, lipid and protein in humans integrating physiology, biochemistry, and nutrition with a focus on maintaining optimal health and preventing disease.|
|A Journey Through the Digestive Tract. Dr. Buhman teaches about the small intestine in digestion and absorption.|
|NUTR 59000||Lipid Regulation and Cell Function: Introduction of hot topics in the area of lipid regulation and cell function and critical evaluation of scientific literature.|
|NUTR 59000||Scientific Writing in Nutrition Science|
|NUTR 60500||Nutritional Physiology and Biochemistry I: Integration of biochemical and physiological functions of nutrients in humans and animals. Dr. Buhman teaches a section related to digestion and absorption of carbohydrates and lipids.|
|NUTR 60700||Nutritional Physiology and Biochemistry II: Integration of biochemical and physiological functions of nutrients in humans and animals, emphasizing transport and metabolism in the context of cardiovascular function. Dr. Buhman teaches a section related to lipoprotein metabolism.|
Office: STON 210
Address: 700 West State Street
West Lafayette, IN 47907