Interdisciplinary Life Science - PULSe Great research is a matter of choice

Wen Jiang

Wen Jiang Profile Picture

Professor

HOCK 131


Contact Info:

jiang12@purdue.edu
765-496-8436

Personal Site: Link


Training Group(s):
Computational and Systems Biology
Immunology and Infectious Diseases
Biomolecular Structure and Biophysics


Current Research Interests:

Electron cryomicroscopy (cryo-EM) is an emerging technique for solving structures of large macromolecular machines. It does not require crystalline order for the studied specimens. This unique capability makes cryo-EM extremely valuable in imaging and solving the structures and the dynamics of macromolecular machines.

We use cryo-EM to study the assembly, maturation, and infection mechanism of viruses including dsDNA tailed bacteriophages and noroviruses. We are also interested in the development of cryo-EM techniques including image processing algorithms, high performance computing, data collection automation, and sample preparation. The technical advances will help push cryo-EM towards near atomic resolution (2-4 Angstrom) single particle 3-D reconstructions and tomographic 3-D reconstructions, and to ultimately transform the current laborious cryo-EM technique with steep learning curve into an easily accessible and high throughput tool at the disposal of any biologist.

Education

Ph.D., Baylor College of Medicine, 2001
M.S., Chinese Academy of Sciences, 1995
B.S., Peking University, 1992

Professional Faculty Research

(single particle cryo-EM, electron crystallography, electron tomography, biophysics) Structure of viruses, macromolecular complexes, and nano-particles; technique developments in cryo-EM for structural determination of macromolecular machines at atomic resolutions; image processing and 3-D reconstruction; computational biology



Selected Publications:

He, Y., Ye, T., Su, M., Zhang, C., Ribbe, A. E., Jiang, W. and Mao, C. 2008. Hierarchical Self-Assembly of Symmetric Supramolecular Polyhedra. Nature. 452(7183):198-201

Jiang, W., Baker, M. L., Jakana, J., Weigele, P., King, J. and Chiu, W. 2008. Backbone Structure of the Infectious Epsilon15 Virus Capsid Revealed by Electron Cryomicroscopy. Nature. 451(7182): 1130-4.

Liu, X., Jiang, W., Jakana, J. and Chiu, W. 2007. Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a multi-path simulated annealing optimization algorithm. J. Struct. Biol. 160(1):11-27

Chang, J., P. Weigele, J. King, W. Chiu, W. Jiang. 2006. Cryo-EM Asymmetric Reconstruction of Bacteriophage P22 Reveals Organization of its DNA Packaging and Infecting Machinery. Structure. 14:1-10.

Jiang, W., Chang, J., Jakana, J., Weigele, P., King, J. and Chiu, W. 2006. Structure of Epsilon15 phage reveals organization of genome and DNA packaging/injection apparatus. Nature.

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