Leifu Chang

Active Mentor - currently hosting PULSe students for laboratory rotations and recruiting PULSe students into the laboratory; serves on preliminary exam committees

Current Research Interests:

Cryo-EM, a technique that wins Nobel Prize in Chemistry 2017, has become a mainstream for structure determination of macromolecular complexes due to a recent resolution revolution. Dr. Chang combines cryo-EM and biochemical reconstitution approach to understand the molecular mechanism of large protein complexes, focusing on those in cell cycle regulation.

Selected Publications:

C. Alfieri, L. Chang, and D. Barford, Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13, Nature (2018):1.

C. Alfieri, L. Chang, Z. Zhang, J. Yang, S. Maslen, M Skehel and D. Barford, Structural basis of APC/C regulation by the spindle assembly checkpoint, Nature, 536 (2016) 431- 436.

S. Zhang, L. Chang, C. Alfieri, Z. Zhang, J. Yang, S. Maslen, M Skehel and D. Barford, Molecular mechanism of APC/C activation by mitotic phosphorylation, Nature, 533 (2016) 260-264.

L. Chang, Z. Zhang, J. Yang, S.H. McLaughlin, D. Barford, Atomic structure of the APC/C and its mechanism of protein ubiquitination, Nature, 522 (2015) 450-454.

L. Chang, X. Liu*, Y. Li, C.C. Liu, F. Yang, J. Zhao and S.F. Sui, Structural organisation of an intact phycobilisome and its association with photosystem II, Cell research, 25 (2015) 726-737

L. Chang, D. Barford, Insights into the anaphase-promoting complex: a molecular machine that regulates mitosis, Current opinion in structural biology, 29C (2014) 1-9.

L. Chang, Z. Zhang, J. Yang, S.H. McLaughlin, D. Barford, Molecular architecture and mechanism of the anaphase-promoting complex, Nature, 513 (2014) 388-393.

L.F. Chang, S. Chen, C.C. Liu, X. Pan, J. Jiang, X.C. Bai, X. Xie, H.W. Wang, S.F. Sui, Structural characterization of full-length NSF and 20S particles, Nature structural & molecular biology, 19 (2012) 268-275.