Interdisciplinary Life Science - PULSe Great research is a matter of choice

Stanton S. Gelvin

Stanton S. Gelvin Profile Picture
H. Edwin Umbarger Distinguished Professor of Biological Sciences
Ph.D., UC San Diego, 1977

Contact Info:

Training Group(s):
Chromatin and Regulation of Gene Expression
Integrative Plant Sciences
Immunology and Infectious Diseases

Current Research Interests:

Crown gall is a neoplastic disease caused by the infection of dicotyledonous plants by virulent stains of the Gram-negative soil bacterium Agrobacterium tumefaciens. During the process of infection part of a bacterial plasmid, called the tumor inducing (Ti) plasmid, is transferred from the bacterium to the plant, where it stably integrates into the nuclear DNA. This transferred, or T-DNA, can be expressed as mRNAs which are translated. Tumorous lesions result, as well as the production of rare compounds called opines which the bacterium can utilize as an energy source. In our laboratory we have been interested in the molecular mechanism of Ti-plasmid transfer, integration, and expression. To define these mechanisms, we are investigating both Agrobacterium and plant genes required for transformation. Among the many approaches we are taking is the identification of Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants) and Arabidopsis mutants that are hyper-susceptible to Agrobacterium transformation (hat mutants). We have identified more than 125 rat mutants and 10 hat mutants are analyzing the functions of the mutated genes in the transformation process. Many of these mutations are in genes involved in chromatin function, nuclear targeting, cytoplasmic trafficking via the actin cytoskeleton, and cell wall biosynthesis. In the course of characterizing plant proteins involved in transformation, we have developed a novel system to image the interaction of Agrobacterium virulence proteins with plant proteins. This system, bimolecular fluorescence complementation, allows one to visualize protein-protein interactions in living plant cells. Finally, we have used microarray and bioinformatic analyses to identify plant genes that respond to Agrobacterium infection. Many of these genes are involved in host defense responses.

Selected Publications:

Tao, Y., Rao, P.K., Bhattacharjee, S. and Gelvin, S.B. 2004. Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2. Proc. Natl. Acad. Sci. USA 101: 5164-5169.

Gaspar, Y.M., Nam, J., Schultz, C.J., Lee, L.-Y., Gilson, P., Gelvin, S.B., and Bacic, A. 2004. Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of Agrobacterium transformation. Plant Physiol.135: 2162-2171.

Lee, L.-Y., and Gelvin, S.B. 2004. Osa protein constitutes a strong oncogenic suppression system that can block vir-dependent transfer of IncQ plasmids between Agrobacterium cells, and the transfer of T-DNA and IncQ plasmids to plant cells. J. Bacteriol. 186: 7254-7261.

Hwang, H.-H, and Gelvin, S.B. 2004. Plant proteins that interact with VirB2, the Agrobacterium pilin protein, are required for plant transformation. Plant Cell 16: 3148-3167.

Gelvin, S.B. 2005. Gene exchange by design (News and Views). Nature 433: 583-584.

Gelvin, S.B. 2005. Viral-mediated plant transformation gets a boost (News and Views). Nature Biotechnol. 23: 684-685.

Yi, H., Sardesai, N., Fujinuma, T., Chan, C.-W., Veena, and Gelvin, S.B. 2006. Constitutive expression exposes functional redundancy between the Arabidopsis histone H2A gene HTA1 and other H2A gene family members. Plant Cell 18: 1575-1589.

Citovsky, V., Lee, L-Yi., Vyas, S., Glick, E., Chen, M.-H., Vainstein, A., Gafni, Y., Gelvin, S.B., and Tzfira, T. 2006. Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J. Mol. Biol. 362: 1120-1131.

Hwang, H.-H., Mysore, K.S. and Gelvin, S.B. 2006. Transgenic Arabidopsis plants expressing Agrobacterium tumefaciens VirD2 protein are less susceptible to Agrobacterium transformation. Mol. Plant Pathol. 6: 743-754.

Gelvin, S.B. 2006. Agrobacterium virulence gene induction. In Methods in Molecular Biology: Agrobacterium protocols. (K. Wang, ed.). Humana Press, Totowa, NJ. Vol. 44. pp. 77-84.

Gelvin, S.B. 2006. Agrobacterium transformation of Arabidopsis thaliana roots: A quantitative assay. In Methods in Molecular Biology: Agrobacterium protocols. (K. Wang, ed.). Humana Press, Totowa, NJ. Vol. 44. pp. 105-113.

Gelvin, S.B. 2006. Using BY-2 cells to investigate Agrobacterium-plant interactions. In Biotechnology in Agriculture and Forestry: Tobacco BY-2 cells: From cellular dynamics to omics. eds. T. Nagata, K. Matsuoka, and D. Inze. Springer (Berlin, Heidelberg, New York). vol. 58, pp. 195-206.

Gelvin, S.B., and Kim, S.-I. 2007. Effect of chromatin upon Agrobacterium T-DNA integration and transgene expression. Biochim. Biophys. Acta 1769: 410-421.

Kim, S.-I., Veena, and Gelvin, S.B. 2007. Genome-wide analysis of Agrobacterium T-DNA integration sites in the Arabidopsis genome generated under non-selective conditions. Plant J. 51: 779-791.

Crane, Y.M., and Gelvin, S.B. 2007. RNAi-mediated gene silencing reveals involvement of Arabidopsis chromatin genes in Agrobacterium-mediated transformation. Proc. Natl. Acad. Sci. USA. 104: 15156-15161.

Lee, L.-Y., Kononov, M.E., Bassuner, B., Frame, B.R., Wang, K., and Gelvin, S.B. 2007. Novel plant transformation vectors containing the super-promoter. Plant Physiol. 145: 1294-1300.

Gelvin, S.B. 2008. Function of host proteins in the Agrobacterium-mediated plant transformation process. In Agrobacterium, from biology to biotechnology (T. Tzfira and V. Citovsky, eds.). Springer, N.Y. 483-522.

Lee, L.-Y., and Gelvin, S.B. 2008. T-DNA binary vectors and systems. Plant Physiol. 146: 325-332.

Gelvin, S.B. 2008. Agrobacterium-mediated DNA transfer, and then some. Nature Biotechnol. (News and Views) 26: 998-1000.

Bhattacharjee, S., Lee, L.-Y., Oltmanns, H. Cao, H., Veena, Cuperus, J., and Gelvin, S.B. 2008. AtImpa-4, an Arabidopsis importin ± isoform, is preferentially involved in Agrobacterium-mediated plant transformation. Plant Cell 20: 2661-2680.

Lee, L.-Y., Fang, M.-J., Kuang, L.-Y., and Gelvin, S.B. 2008. Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells. Plant Methods. 4: 24. doi:10.1186/1746-4811-4-24.

Hodges, L.D., Lee, L.-Y., McNett, H., Gelvin, S.B., and Ream, W. 2009. Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for transformation of plants. J. Bacteriol. 191: 365-374.

Gelvin, S.B. 2009. Agrobacterium in the genomics age. Plant Physiol. 150: 1665-1676.

Zheng, Y., He, X.-W., Ying, Y.-H., Lu, J.-F., Gelvin, S.B., and Shou, H.-X. 2009. Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency. Mol. Plant. doi: 10.1093/mp/ssp038.

Tenea, G.N., Spantzel, J., Lee, L.-Y., Zhu, Y., Lin, K., Johnson, S.J., and Gelvin, S.B. 2009. Over-expression of several Arabidopsis histone genes increases Agrobacterium-mediated transformation and transgene expression in plant cells. Plant Cell. 21: 3350-3367.

G.R. Olbricht, N. Sardesai, S.B. Gelvin, B.A. Craig, and R.W. Doerge. 2009. Statistical methods for Affymetrix tiling array data. The Proceedings of the Kansas State University Conference on Applied Statistics in Agriculture. Manhattan, KS. (in press)

Oltmanns,H., Frame, B., Lee, L.-Y., Johnson, S., Li, B., Wang, K., and Gelvin, S.B. 2009. Generation of backbone free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome. Plant Physiol.

Gelvin, S.B. 2009. Finding a way to the nucleus. Current Opin. Microbiol. DOI 10.1016/j.mib.2009.11.003.

Gelvin, S.B. 2010. Finding a way to the nucleus. Current Opin. Microbiol. 13: 53-58. DOI 10.1016/j.mib.2009.11.003.

Gelvin, S.B. 2010. Plant proteins involved in Agrobacterium-mediated genetic transformation. Annu. Rev. Phytopathol. 48: 45–68. doi: 10.1146/annurev-phyto-080508-081852.

Gelvin, S.B. 2010. Manipulating plant histone genes to improve Agrobacterium- and direct DNA-mediated plant genetic transformation. Inform. Systems Biotechnol. News Rep. March, 2010.

Gelvin, S.B. 2010. Improving the quality of plant transformation events. ISB News Rep. July, 2010.

Gelvin, S.B. 2012. Traversing the cell: Agrobacterium T-DNA’s journey to the host genome. Front. Plant Sci. 3:52. doi: 10.3389/fpls.2012.00052.

Lee, L.-Y., Wu, F.-H., Hsu, C.-T., Shen, S.-C., Yeh, H.Y., Liao, D.-C., Fang, M.-J., Liu, N.-T., Yen, Y.-C., Dokládal, L., Sýkorová, E., Gelvin, S.B., and Lin, C.-S. 2012. Screening a cDNA library for protein-protein interactions directly in planta. Plant Cell. doi/10.1105/tpc.112.097998.

Lee, L.Y., and Gelvin, S.B. 2012. Bimolecular fluorescence complementation for imaging protein-protein interactions in planta. In Methods in Molecular Biology: Plant Functional Genomics. (P. Springer, ed.). Humana Press, Totowa, NJ. In press.

Sardesai, N., Lee, L.-Y., Chen, H., Yi, H.-C., Olbricht, G.R., Stirnberg, A., Jeffries, J., Xiong, K., Doerge, R.W., and Gelvin, S.B. 2013. A myb transcription factor regulates Agrobacterium transformation via cytokinin signaling. Science Signaling 6: (302), ra100. [DOI: 10.1126/scisignal.2004518]. This article was featured both in a Science podcast and in a journal cover photo. The article was selected by The Latest Science for its impact on scientific knowledge.

Sardesai, N., Laluk, K., Mengiste, T., and Gelvin, S.B. 2014. The Arabidopsis Myb transcription factor MTF1 is a unidirectional regulator of susceptibility to Agrobacterium. Plant Signaling and Behavior 9:e28983;

Y., Lee, L.-Y, and Gelvin, S. 2014. Is VIP1 important for Agrobacterium-mediated transformation? Plant J. 79: 848-860. doi: 10.1111/tpj.12596

Park, S.-Y., Yin, X., Duan, K., Gelvin, S.B., and Zhang, Z. 2014. Heat shock protein 90.1 plays a role in Agrobacterium-mediated plant transformation. Mol. Plant. 7: 1793-1796. doi: 10.1093/mp/ssu091.

Park, S.-Y., Vaghchhipawala, Z., Vasudevan, B., Lee, L.-Y., Shen, Y., Singer, K., Waterworth, W.M., Zhang, Z., West, C.E., Mysore, K.S., and Gelvin, S.B. 2015. Agrobacterium T-DNA integration into the plant genome can occur without the activity of key non-homologous end-joining proteins. Plant J. 81: 934–946. doi: 10.1111/tpj.12779.

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