Interdisciplinary Life Science - PULSe Great research is a matter of choice

Stanton S. Gelvin

Stanton S. Gelvin Profile Picture
H. Edwin Umbarger Distinguished Professor of Biological Sciences
Ph.D., UC San Diego, 1977

Contact Info:
gelvin@purdue.edu
765-494-4939

Training Group(s):
Chromatin and Regulation of Gene Expression
Integrative Plant Sciences
Microbiology
Immunology and Infectious Diseases


Current Research Interests:

Crown gall is a neoplastic disease caused by the infection of dicotyledonous plants by virulent stains of the Gram-negative soil bacterium Agrobacterium tumefaciens. During the process of infection part of a bacterial plasmid, called the tumor inducing (Ti) plasmid, is transferred from the bacterium to the plant, where it stably integrates into the nuclear DNA. This transferred, or T-DNA, can be expressed as mRNAs which are translated. Tumorous lesions result, as well as the production of rare compounds called opines which the bacterium can utilize as an energy source. In our laboratory we have been interested in the molecular mechanism of Ti-plasmid transfer, integration, and expression. To define these mechanisms, we are investigating both Agrobacterium and plant genes required for transformation. Among the many approaches we are taking is the identification of Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants) and Arabidopsis mutants that are hyper-susceptible to Agrobacterium transformation (hat mutants). We have identified more than 125 rat mutants and 10 hat mutants are analyzing the functions of the mutated genes in the transformation process. Many of these mutations are in genes involved in chromatin function, nuclear targeting, cytoplasmic trafficking via the actin cytoskeleton, and cell wall biosynthesis. In the course of characterizing plant proteins involved in transformation, we have developed a novel system to image the interaction of Agrobacterium virulence proteins with plant proteins. This system, bimolecular fluorescence complementation, allows one to visualize protein-protein interactions in living plant cells. Finally, we have used microarray and bioinformatic analyses to identify plant genes that respond to Agrobacterium infection. Many of these genes are involved in host defense responses.



Selected Publications:

Altpeter, F., Springer, N.M., Bartley, L.E., Blechl, A.E., Brutnell, T.P., Citovsky, V., Conrad, L.J., Gelvin, S.B., Jackson, D.P., Kausch, A.P., Lemaux, P.G., Medford, J.I., Orozco-Cárdenas, M.L., Tricoli, D.M., Van Eck, J., Voytas, D.F., Walbot, V., Wang, K., Zhang, Z.J.,and Stewart, C.N. 2016. Advancing Crop Transformation in the Era of Genome Editing. Plant Cell. doi 10.1105/tpc.16.00196.

Wei, F.-J., Kuang, L.-Y., Oung, H.-M., Cheng, S.-Y., Wu, H.-P., Huang, L.-T., Tseng, Y.-T., Chiou, W.-Y., Hsieh-Feng, V., Chung, C.-H., Yu, S.-M., Lee, L.-Y., Gelvin, S.B., and Hsing, Y.-I.C. 2016. Somaclonal variation does not preclude using rice transformants for genetic screening. Plant J. 85:648-659. DOI: 10.1111/tpj.13132.

Dokládal, L., Honys, D., Rana, R., Lee, L.-Y., Gelvin, S.B., and Sýkorová, E. 2015. cDNA library screening identifies protein interactors potentially involved in non-telomeric roles of Arabidopsis telomerase. Front. Plant Sci. 6:985. Doi:10.3389/fpls.2015.00985.

Park, S.-Y., Vaghchhipawala, Z., Vasudevan, B., Lee, L.-Y., Shen, Y., Singer, K., Waterworth, W.M., Zhang, Z., West, C.E., Mysore, K.S., and Gelvin, S.B. 2015. Agrobacterium T-DNA integration into the plant genome can occur without the activity of key non-homologous end-joining proteins. Plant J. 81: 934–946. doi: 10.1111/tpj.12779.

Hwang, H.-H., Gelvin, S.B., and Lai, E.-M. 2015. Editorial: “Agrobacterium biology and its application to transgenic plant production”. Front. Plant Sci. 6:265. doi. 10.3389/fpls.2015.00265.

Sardesai, N., Laluk, K., Mengiste, T., and Gelvin, S.B. 2014. The Arabidopsis Myb transcription factor MTF1 is a unidirectional regulator of susceptibility to Agrobacterium. Plant Signaling and Behavior 9:e28983; http://dx.doi.org/10.4161/psb.28983

Shi, Y., Lee, L.-Y, and Gelvin, S. 2014. Is VIP1 important for Agrobacterium-mediated transformation? Plant J. 79: 848-860. doi: 10.1111/tpj.12596

Park, S.-Y., Yin, X., Duan, K., Gelvin, S.B., and Zhang, Z. 2014. Heat shock protein 90.1 plays a role in Agrobacterium-mediated plant transformation. Mol. Plant. 7: 1793-1796. doi: 10.1093/mp/ssu091.

Gelvin, S.B. 2012. Traversing the cell: Agrobacterium T-DNA’s journey to the host genome. Front. Plant Sci. 3:52. doi: 10.3389/fpls.2012.00052.

Lee, L.-Y., Wu, F.-H., Hsu, C.-T., Shen, S.-C., Yeh, H.Y., Liao, D.-C., Fang, M.-J., Liu, N.-T., Yen, Y.-C., Dokládal, L., Sýkorová, E., Gelvin, S.B., and Lin, C.-S. 2012. Screening a cDNA library for protein-protein interactions directly in planta. Plant Cell. 24: 1746-1759. doi/10.1105/tpc.112.097998.

Sardesai, N., Lee, L.-Y., Chen, H., Yi, H.-C., Olbricht, G.R., Stirnberg, A., Jeffries, J., Xiong, K., Doerge, R.W., and Gelvin, S.B. 2013. A myb transcription factor regulates Agrobacterium transformation via cytokinin signaling. Science Signaling 6: (302), ra100. [DOI: 10.1126/scisignal.2004518]. This article was featured both in a Science podcast and in a journal cover photo. The article was selected by The Latest Science for its impact on scientific knowledge.

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