Two-dimensional gel electrophoresis (2DGE) is a method of protein separation by which proteins in a mixture are separated according to their isoelectric point (pI) in the horizontal direction (isoelectric focusing [IEF]) and their molecular weight in the vertical direction (sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]). 2DGE is used for the isolation/separation/purification of proteins for further characterization with mass spectrometry and identification of specific proteins. The isoforms of a protein can easily be isolated with 2DGE.

2DGE services require about 4-7 business days.

Samples should be free from salts or ionic detergents which might interfere with isoelectric focusing.  Protein concentration should be recorded on the sample request form.

Costs

To enable efficiency in the facility, samples for 2-D Gel will be processed ONLY on the first and third Tuesday of every month. A minimum of 5 samples will be required to trigger each biweekly run. If the run will not occur because of insufficient numbers of sample, the requesting researcher will be notified of the delay. Rates are based on the operator and equipment use required for these analyses

“Rush” sample analysis will be subject to the normal hourly rate.

Internal Rate:
$50.00 per sample.

External Rate:
$83.00 per sample

Download the Protein Sequence Analysis Request Form here and return it to the Purdue Proteomics Facility with your sample(s) to be sequenced.
The Protein Sequence Analysis Request Form will require a valid account number, your name, your telephone number, and information about the sample, including how far you want it sequenced (note: you will get one amino acid per cycle).  If you are just checking to see if you have isolated the desired known, indicate just 5 cycles.  If this is a precious and unknown sample, just say "All Possible,” and as much readable sequence as possible will be attempted.