Birck Nanotechnology Center

Operating Policies and Procedures

Laboratories Procedures

SPECIFIC TO THE LABORATORIES ONLY

THE BIRCK NANOTECHNOLOGY CENTER CONTAINS GENERAL LABORATORIES AND BIOSAFETY LEVEL 2 (BSL 2) LABORATORIES.  THERE ARE NO BIOSAFETY LEVEL 3 (BSL 3) LABORATORIES IN THE BNC.

5.1 General Laboratory Procedures (Apply to All Laboratories)

5.2 Biolaboratories

5.3 Laser Laboratories

5.4 High Voltage / X-Ray Laboratories

5.5 Laboratory Areas With Rotating Equipment

5.6 Laboratory Service Galleys


5.1 General Laboratory Procedures (Apply to All Laboratories)


5.1.1 General Policies

  • Lab Training is required for unescorted access-- Badges must be worn at all times.

  • BNC Visitors must be escorted at all times.  The host is responsible for visitor’s behavior.

  • Doors must be closed and locked at all times.  (DO NOT prop doors open.)

  • No eating, drinking, or chewing gum in research areas including the labs and support galleys.

  • All users must abide by the Glove Policy (see Section 5.1.4).

     


5.1.2 Clothing Requirements

  • Fully enclosed shoes must be worn in all laboratories.  No open-toe or open-heel shoes are allowed.
  • Long pants or long skirt must be worn in all laboratories – no shorts are allowed.
  • Glasses must be worn in all laboratories.  Safety glasses with side shields are strongly recommended

5.1.3 Labs Containing Compressed Gases

Glasses or safety glasses are required in laboratories where a compressed gas that discharges into the room (not into equipment) and/or a blow-off gun is used.  Safety glasses with side shields are strongly recommended.
Requirements for “All Laboratories” (Section 5.1.1) also apply.


5.1.4 Glove Policy

Laboratory clean gloves (Blue):  Must be worn in laboratories when working with the interior of equipment, handling contamination-sensitive parts, or other conditions where skin oils would be a problem.  Blue gloves may NOT be used for handling chemicals (except solvent squirt bottles).
Laboratory chemical gloves (Orange):  Should always be worn within hazardous zone at hood.  Orange gloves should never worn outside of hazardous zone EXCEPT when transporting or handling chemicals
Biological gloves (Cream):  Can ONLY be worn within BSL1-rated laboratories.
Black – Can ONLY be worn within BSL2-rated laboratories.  No gloves allowed:  On doorknobs/doorhandles, computers, telephones, or in offices.
“One-glove rule” is acceptable.  This means that one hand is gloved for handling sample, while the other hand is not gloved, used for door knobs, etc.


5.1.5 Hood Hazard Zones

Fume Hood Hazard Zones are demarcated with red and yellow hazard tape in each Birck laboratory.  Within these hazard zones, specific policies must be followed.


5.1.5.1 Solvent Hood Hazardous Zone

Requirements for “All Laboratories” apply (Section 5.1.1).  Furthermore, solvent hood specific Personal Protective Equipment (PPE) is required in hazardous zone.  PPE includes:

  • Non-vented chemical-proof safety goggles
  • Chemical-resistant coat-apron
  • Chemical-resistant gloves

Users must adhere to the Buddy Policy (Section 5.1.7) to work in a solvent hood.

Note: The Hazardous Zone is marked with tape


5.1.5.2 Acid Hood Hazardous Zone

Requirements for “All Laboratories” apply (Section 5.1.1).  Furthermore, acid hood specific Personal Protective Equipment (PPE) is required in hazardous zone.  PPE includes:

  • Non-vented chemical-proof safety goggles
  • Chemical-resistant coat-apron
  • Chemical-resistant gloves

When pouring chemicals in hazardous zone.  PPE includes:

  • All PPE required above
  • Face shield

Users must adhere to the Buddy Policy (Section 5.1.7) to work in an acid hood.
NOTE: The Hazardous Zone is marked with tape.


5.1.6 Chemical Hazard Zones


5.1.6.1 Chemical Squirt Bottles

A limited number of chemicals are approved for use in squirt bottles, outside of chemical hoods.
Requirements for “All Laboratories” apply (Section 5.1.1).  Furthermore, non-vented chemical proof safety goggles are required when handling squirt bottles outside of hood.


5.1.6.2 Transporting Chemicals

Requirements for “All Laboratories”, plus: Appropriate PPE (solvent or acid) while packing/unpacking chemicals.
All chemicals being transported outside of laboratories or within the cleanroom – including in the galleys – must be doubly contained in an approved safety overpack, or a spill-proof outer container.
No more than 1 gallon may be transported without use of a cart.
Goggles are required while transporting hazardous chemicals.


5.1.6.3 Toxic Materials

Requirements for “All Laboratories” apply, plus:
Cyanide and cyanide solutions MAY NOT be used in an acid hood or in proximity to acids!  Lethal gases could be released.
When there is a potential for exposure to toxic materials – e.g., opening a chamber or process line – check with a staff member BEFORE proceeding.  PPE according to the MSDS for the material must be used. 
Read and understand the hazards of all materials that are being used in the lab!
Buddy Policy applies here (see Section 5.1.7).


5.1.7 Buddy Policy

When working with hazardous materials (see definition Section 3) you must have a second BNC user or lab buddy present:
You may work alone:  During normal working hours: 7am-5pm Monday-Friday excluding holidays and breaks, or when  lab attendants are on duty in the building (nominal hours listed below, holidays and breaks excluded).   Note that the lab attendants will circulate through the building during the following weekly hours:
Mon -Fri         5pm – 12midnight
Sat & Sun        noon – 12midnight
At all other times, you must have another authorized user in the same lab.
Changes and holiday exclusions to lab attendants’ hours will be posted on the website and/or in the building.


5.2 Biolaboratories

The BNC Biolaboratories are classified as General Biological Laboratories - Biosafety Level 1 (BSL1) and Biosafety Level 2 (BSL 2) Laboratories.  There are no BSL3 classified laboratories in the BNC.
All laboratory personnel and others whose work requires them to enter the laboratory must understand the biological and other hazards with which they will come in contact through their normal work in the laboratory, and be trained in appropriate safety precautions and procedures.  Personnel must be required to know, understand, and follow standard practices and procedures. Training in laboratory safety must be provided and competence in safe technique demonstrated before work is allowed with hazardous agents or toxins.


5.2.1 General Biological Laboratories

Laboratories, 2077, and 2081 in the BNC are general biological laboratories.  These laboratories are classified as BioSafety Level 1 (BSL1).
General biological laboratories are suitable for experiments involving agents of no known or minimally potential hazard to laboratory personnel and the environment.  Appropriate PPE must be worn (lab coat optional) but work is generally conducted on open bench tops. Special containment equipment is not required or generally used.


BSL1 Training for General Biological Labs:
Laboratory personnel must have specific training in the procedures conducted in the laboratory and are supervised by a scientist with general training in microbiology or a related science.   Mandatory training involves, (a) Online Biosafety training for Birck Labs (BNC-208) that must be taken and the online test must be passed,  (b) REM online biosafety training that must also be taken and all tests must be passed, and (c) any other biosafety training required by the Primary Investigator and the Birck Biosafety Officer that must be completed.  The following standard and special practices apply to agents assigned to general biological laboratories.


5.2.1.1 Standard Microbiological Practices

  1. Laboratory doors are kept closed when experiments are in progress.
  2. Work surfaces are decontaminated daily and after any spill of biohazardous material.
  3. All contaminated liquid or solid wastes are decontaminated before being disposed of or otherwise handled.
  4. Mechanical pipetting devices are used; mouth pipetting is prohibited.
  5. Eating, drinking, smoking, storing of food, and applying cosmetics are not permitted in the work area.
  6. Persons wash their hands after they handle biohazardous materials and when they leave the laboratory. 
  7. All procedures must be carefully performed to minimize the creation of aerosols.

5.2.1.2 Personal Protective Equipment and Laboratory Gowning (clothing) Requirements for BSL-1

  1. The wearing of laboratory coats is recommended.
  2. Gloves are required.
  3. Do not touch door knobs or telephones with gloved hands.
  4. Before leaving laboratory, remove and discard gloves, and wash hands. 

5.1.2.3 Special Practices

Contaminated Materials:  Contaminated materials that are to be decontaminated (autoclaved) at a site away from the laboratory are placed in an orange biohazard bag that in turn is placed inside a durable leak-proof container.  In the Birck BSL2 labs these containers are white trash cans lined with the orange plastic biohazard bags. All contaminated solid waste should be placed in these containers and kept covered.  BNC Staff will enter the lab weekly to transfer this waste to the large orange disposal cans located in the galley next to the lab doors.  Separate biohazardous trash containers are available for liquid waste while red sharps containers are for the containment of contaminated sharps and glass in the BNC Biolaboratories.
Contaminated red sharps boxes will be autoclaved in Bindley Bioscience Center, Room 233, after they are transported in the above trash cans. BNC staff in charge of this process will make sure that autoclaving is performed successfully. Decontaminated bags are removed from the autoclave and properly disposed of to await REM pickup in a biohazard cardboard box. When the box is full, the box is taped up and a REM Biohazardous Waste Pickup form is filled in and attached to the top of the box. REM is notified by phone in advance so that REM can dispose of this decontaminated trash as soon as possible.  

 

5.1.2.4 Containment Equipment

Special containment equipment is generally not required for manipulations of agents assigned to general biological laboratories.


5.2.2 Biosafety Level 2 (BSL 2) Laboratories

Biosafety Level 2 laboratories are similar to general biological laboratories and are suitable for work involving agents that represent a moderate hazard for personnel. The environment of  Biosafety Level 2 laboratories differs from BSL1 laboratories in that:

  1. BSL2 Laboratory personnel have specific training in handling pathogenic agents and are directed by the principle investigator.    Online Biosafety training for Birck Labs must be taken and the online test must be passed.  REM online biosafety training must be taken and tests must be passed.  Personnel must be properly trained in the use of biological safety cabinets.  Personnel will be trained for proper biological waste management.
  2. Access to the BSL2 laboratories is limited when work is being conducted, and is restricted to trained users only, when hazardous materials are in use.
  3. All BSL2 laboratory users are responsible for knowing the appropriate procedures contained in the Purdue Biological Safety Manual, found at this link: http://www.purdue.edu/rem/home/booklets/bioman.pdf.   
  4. Certain procedures in which biohazardous aerosols are created need to be conducted in biological safety cabinets or other physical containment equipment.
  5. The following standard and special practices, safety equipment, and facilities apply to agents assigned to Biosafety Level 2:

5.2.2.1 Standard Microbiological Practices

  1. Access to the BSL 2 laboratory is limited or restricted by the supervisor when work with biohazardous agents is in progress. Laboratory doors are kept closed at all times.
  2. Work surfaces are decontaminated at least once a day and after any spill of biohazardous material.
  3. All contaminated liquid or solid waste is decontaminated before disposal.
  4. Mechanical pipetting devices are used; mouth pipetting is prohibited.
  5. Eating, drinking, smoking, chewing gum or tobacco, and applying cosmetics are not permitted in the laboratory.
  6. Persons wash their hands when first entering the lab, after handling biohazardous agents, and when leaving the laboratory. 
  7. All procedures are performed carefully to minimize the creation of aerosols.
  8. Serological procedures with inactivated antigens known or shown to be free of residual infectivity can be performed on the open bench.
  9. Bench top work is done on absorbent paper, which is disposed of using approved biological waste procedures.   (See Section 5.2.2.3)

5.2.2.2 Personal Protective Equipment and Laboratory Gowning (clothing) Requirements for BSL-2

  1. Requirements for “All Laboratories” (Section 5.1.1.).
  2. Specially marked lab coats are required in the BSL 2 laboratory. Laboratory clothing or gloves must not be worn in non-laboratory areas.
  3. No shorts, sandals, or open-toed shoes are allowed in the laboratories.
  4. Surgical mask must be worn for aerosol protection when working with infectious agents.
  5. Birck-designated biological gloves are required.
  6. Laboratory clothing or gloves must not be worn in non-laboratory areas.

5.2.2.3 Special Practices

  1. Contaminated materials that are to be decontaminated or autoclaved away from the BSL2 laboratory are placed in an orange biohazard bag which is then placed inside a durable, leak-proof container, which is closed before being removed from the laboratory.  This outer container must be a water-impervious biohazard container with a biohazard label clearly visible on it.
  2. Access to the BSL2 laboratory is limited by the laboratory supervisor when experiments are being conducted. In general, persons who are at increased risk of acquiring infection or for whom infection may be unusually hazardous are not allowed in the BSL2 laboratory or animal rooms. Persons at increased risk may include children, pregnant women, and individuals who are immunodeficient or immunosuppressed. The supervisor has the final responsibility for assessing each individual circumstance and determining who may enter or work in the area.
  3. The Birck Biosafety Officer will assure that only persons who have been advised of the potential hazard and who meet any specific entry requirements (e.g. immunizations) enter the BSL2 laboratories.
  4. When biohazardous materials are present in the laboratory, a biohazard warning sign incorporating the universal biohazard symbol is posted on all laboratory access doors and on such other items (i.e. equipment, containers, and materials) as appropriate to indicate the presence of biohazardous agents. The hazard warning sign should list the name of the laboratory supervisor or other responsible person(s), and indicate any special requirements for entering the area (immunization, respirators, etc.). 
  5. An insect and rodent control program is in effect.
  6. All wastes from laboratories must be appropriately decontaminated before being disposed. 
  7. If activities of lesser biohazard potential are conducted in the laboratory concurrently with activities requiring Biosafety Level 2, all activities will be conducted at Biosafety Level 2.
  8. Gloves will be worn for all procedures requiring the handling of biohazardous materials.
  9. Serological procedures with inactivated antigens shown to be free of residual infectivity can be performed on the open bench.
  10. All spills, accidents, and overt or potential exposures to biohazardous materials must be immediately reported to the laboratory supervisor. A written record must be prepared and maintained. Appropriate medical evaluation, surveillance, and treatment must be provided.
  11. Serum specimens may be collected periodically depending on the agents handled or the function of the facility.
  12. A safety or operations manual which identifies known and potential hazards and which specifies practices and procedures to minimize or eliminate such risks should be prepared or adopted. Personnel should be advised of special hazards and are required to follow standard practices and procedures.

5.2.2.4 Containment equipment

Biological safety cabinets (Class II) or other appropriate personal protective or physical containment devices are used whenever:

  1. Procedures with a high potential for creating biohazardous aerosols are conducted. These may include centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, and opening containers of biohazardous materials whose internal pressures may be different from ambient pressures, and procedures using animal derived tissue samples.
  2. High concentrations or large volumes of biohazardous agents are used. Such materials may be centrifuged in the open laboratory if sealed heads or centrifuge safety cups are used and if they are opened only in a biological safety cabinet.

5.2.3 Material Transport and Handling

Transport carts are never to be used inside BSL 2 laboratories.  Contaminated materials leaving the labs must be double bagged and carried on carts kept outside the laboratories.
Specific PPE (BSL1 or BSL2), including safety glasses, must be worn while packing/unpacking samples.  Seal all BSL1 and/or BSL-2 samples with an appropriate cap or lid and Parafilm to prevent spillage.  All samples must be placed inside a secondary container that is not breakable (e.g. styrofoam box).  Anything over 5 lbs. should be transported via a lab cart.  All secondary containers will be marked with a biohazard label stating whether the sample is BSL1 or BSL2.  Once sealed, samples will not be opened en route to BSL1 or BSL2 labs.


5.3 Laser Laboratories

When using a class 3b or 4 lasers, the foremost concern is the danger posed to one's eyes and exposed skin.  Depending on the wavelength and the power output of the laser light as well as the exposure duration, the cornea, lens, or retina may be injured.  Irreparable damage to parts of the eye, and permanent partial or full loss of vision are possible. 
Accidental exposure to laser light can be minimized by following the standard operation procedures of the laser system, using correct approved laser safety eyewear.  Requirements for “All Laboratories” apply here (see Section 5.1.1).  Wavelength specific laser safety goggles are required. 
Radiation safety signs must be clearly posted and laser safety curtains must be installed around laser.
Individuals are authorized to use class 3b and 4 lasers upon receiving laser safety training, demonstrating competency, and submitting complete application forms.  Contact REM to learn more about Purdue's laser safety and training program.
All laser documentation must be listed with REM


5.4 High Voltage/X-Ray Laboratories

Baseline monitoring of equipment must be done upon installation.  This must be followed by semi-annual check of X-Ray levels.  Furthermore, individuals are authorized to use BNC X-Ray Diffraction equipment upon completion of radiation safety training, demonstration of competency, and submitting completed forms.  Contact REM to learn more about Purdue’s radiation safety and training program.
Do not remove or modify existing safety measures on all high voltage and/or x-ray equipment.  If any modification is necessary, appropriate supervision and re-monitoring is required.  Parties that must be notified include:

    • Safety Manager
    • Building Manager
    • REM

5.5 Laboratory Areas With Rotating Equipment

Requirements for “All Laboratories” apply (Section 5.1.1) as well as glasses or safety glasses.  Safety glasses with side shields are strongly recommended. 

Loose clothing such as neckties, etc., are to be secured.  Long hair must be secured or covered.

5.6 Laboratory Service Galleys

General Laboratory Policies must be followed in galleys (Section 5.1).
 
Guidelines for areas with rotating equipment apply (Section 5.5)



Facilities