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MCF INSTRUMENTATION

Laser-Enabled Analysis and Processing (LEAP™) Instrument

Inner view of LEAP™ revealing the Nd:YAG pump laser in operation.

LEAP™ uses a Nd:YAG pump diode laser and galvanometer-controlled mirrors to control the laser to ablate (positive or negative single or multiple selection) or opto-inject living cells (Cyntellect, Inc.). (Peter Szaniszlo, William A. Rose, Nan Wang, Lisa M. Reece, Tamara V. Tsulaia, Elie G. Hanania, Cornelis J. Elferink, James F. Leary. Scanning Cytometry with a LEAP: Laser-Enabled Analysis and Processing of Live Cells In Situ. Cytometry; Accepted, 2005)

High Resolution Cell Sorter (HiReCS)

The HiReCS is the fastest high-speed cell sorter in the world (sorts >170,000 cell/sec) utilizing 3 lasers (a water-cooled Spectra-Physics argon ion tunable laser, an iCyt 488 nm solid-state laser, and a Melles-Griot HeNe laser) along with a single layer neural net for two-staged sort decisions. (Leary, J.F. "Ultra High Speed Cell Sorting", Cytometry, Part A 67A: 76-85, 2005)

BioMEMS Microfluidic Cell Sorter Prototype

Data acquisition and BioMEMS prototyping workstation utilizing a National Instruments' PXI chassis and controllers with LabVIEW RT™. The prototype will utilize LEDs and avalance photodiodes to excite fluorochromes on and within a cell and then collect the emitted light and acquire the data. Ultimately, cells of interest will be collected for further molecular analysis.

0.2 and 10 micron fluorescent beads (Duke Scientific, Inc.) flowing through an incubation chamber of a PDMS microchip (courtesy of Rashid Bashir, Ph.D.). The microchip is part of the fluidics system for the BioMEMS microfluidics sorter. A mutlichamber microchip will allow for rapid flow and ultimate separation of cells-of-interest.