Cyber Center

Bioinformatics Seminar

November 6 @ 4:30 PM - 5:30 PM - Lily G126

Date: Tuesday, November 6, 2012
Time: 4:30 PM
Place: LILY G126

Speakers: Joseph Irudayaraj , Department of Biological Engineering, Purdue University

Title: "Single Molecule and single particle technologies in biology"


Abstract

Nanoscale structures and high resolution microscopy have provided both engineers and scientists with a plethora of opportunities to explore biology. Using simple nanomaterials we will show that highly sensitive detection is possible almost at the limit of single molecule resolution. To this end, we will show that Plasmon nanorulers can be used to monitor mRNA in live single cells. These concepts can also be used to detect microRNA as well as protein DNA interactions in live cells. A second example that I would like to focus on involves detecting epigenetic modifications at single nucleosome resolution and in single cell. Highly sensitive methodologies can be used to enhance our understanding of biology which can lead to detecting a disease early in its formation or in detecting targets that otherwise are not possible by conventional means. I will conclude the talk with some perspectives and future implications.

Associated Reading:

1. Kyuwan Lee, Vladimir P. Drachev, and Joseph Irudayaraj. 2011. DNA-Gold Nanoparticle Reversible Networks Grown on Cell Surface Marker Sites: Application in Diagnostics. ACS Nano.  2011 Mar 22;5(3):2109-17. Epub 2011 Feb 11. http://www.stat.purdue.edu/calendar/seminars/bioinformatics/fall-12/lee.pdf   
2. Lan Sun and Joseph Irudayaraj. 2009. Quantitative Surface-Enhanced Raman for Gene Expression Estimation. Biophysical Journal Volume 96:4709-4716.  http://www.stat.purdue.edu/calendar/seminars/bioinformatics/fall-12/sun.pdf   
3. Jiji Chen, Andrew Miller, Ann L. Kirchmaier and Joseph M. K. Irudayaraj. 2012. Single Molecule Tools Elucidate H2A.Z Nucleosome Composition.JCS online publication date 5 March  2012. http://www.stat.purdue.edu/calendar/seminars/bioinformatics/fall-12/chen.pdf

 

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